Effects of arginine on the growth and microcystin-LR production of Microcystis aeruginosa in culture

Dai, Ruwei; Zhou, Yanping; Chen, Yanan; Zhang, Xufeng; Yan, Yangwei; An, Dong Sung

doi:10.1016/j.scitotenv.2018.09.213

Cited by 17 publications

(6 citation statements)

References 60 publications

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“…(Dai et al ., ), and intracellular amino acid availability has been linked to both cyanophycin and MC production in Planktothrix , another toxic bloom forming cyanobacteria (Van de Waal et al ., ). The specific addition of arginine seems to be important, and it was discovered to be the last amino acid added to the MC molecule (Dai et al ., ). Therefore, this study supports that there is likely a link between MC and arginine production, and the production of arginine itself may be a limiting step in the synthesis of MC.…”

Section: Discussionmentioning

confidence: 97%

Nitrogen flux into metabolites and microcystins changes in response to different nitrogen sources inMicrocystis aeruginosaNIES‐843

Krausfeldt

Farmer

Castro

et al. 2020

Environmental Microbiology

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The over-enrichment of nitrogen (N) in the environment has contributed to severe and recurring harmful cyanobacterial blooms, especially by the non-N 2fixing Microcystis spp. N chemical speciation influences cyanobacterial growth, persistence and the production of the hepatotoxin microcystin, but the physiological mechanisms to explain these observations remain unresolved. Stable-labelled isotopes and metabolomics were employed to address the influence of nitrate, ammonium, and urea on cellular physiology and production of microcystins in Microcystis aeruginosa NIES-843. Global metabolic changes were driven by both N speciation and diel cycling. Tracing 15 N-labelled nitrate, ammonium, and urea through the metabolome revealed N uptake, regardless of species, was linked to C assimilation. The production of amino acids, like arginine, and other N-rich compounds corresponded with greater turnover of microcystins in cells grown on urea compared to nitrate and ammonium. However, 15 N was incorporated into microcystins from all N sources. The differences in N flux were attributed to the energetic efficiency of growth on each N source. While N in general plays an important role in sustaining biomass, these data show that N-speciation induces physiological changes that culminate in differences in global metabolism, cellular microcystin quotas and congener composition.

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Section: Discussionmentioning

confidence: 97%

Nitrogen flux into metabolites and microcystins changes in response to different nitrogen sources inMicrocystis aeruginosaNIES‐843

Krausfeldt

Farmer

Castro

et al. 2020

Environmental Microbiology

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“…Jian; 5.5% of dietary protein), juvenile grass carp (Ctenopharyngodon idella; 5.71% of dietary protein) [38], and blunt snout bream (Megalobrama amblycephala; 5.58% of dietary protein) [14]; higher than that of hybrid striped bass (Morone chrysops × saxatilis; 4.4% of dietary protein) [39], American redfish (Sciaenops ocellatus; 5.0% of dietary protein) [40], and Atlantic salmon (Salmo salar; 4.8% of dietary protein) [41]; and lower than that of grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂; 6.64-6.82% of dietary protein) [42] and black sea bream (Sparus macrocephalus; 7.74% of dietary protein) [43]. Some studies have shown that the arginine requirement of O. mykiss is 5.9% protein by growth [44], which is slightly lower than that of O. mykiss obtained in this study (6.52% of dietary protein). The different arginine requirements obtained from the above studies may be due to the species and size of the experimental fish, composition of base feed (including purified, semipurified, and practical feed), experimental design, culture conditions, etc.…”

Section: Effects Of Dietary Arginine Levels On Growthmentioning

confidence: 99%

“…For example, fish in this experiment were triploid O. mykiss and the feed was supplemented with 15% low fishmeal. In addition, the absorption 7 Aquaculture Nutrition rate and bioavailability efficiency of arginine can affect the requirements [44]. When dietary arginine reaches or exceeds 2.71%, the rate of increase in gain rate, SGR, and PER decreases.…”

Section: Effects Of Dietary Arginine Levels On Growthmentioning

confidence: 99%

Effects of Dietary Arginine on Growth Performance, Digestion, Absorption Ability, Antioxidant Capability, Gene Expression of Intestinal Protein Synthesis, and Inflammation-Related Genes of Triploid Juvenile Oncorhynchus mykiss Fed a Low-Fishmeal Diet

Wang

Liu

et al. 2022

Aquaculture Nutrition

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In this study, the effects of arginine on growth, serum antioxidant levels, intestinal digestion physiology, intestinal protein synthesis, and expression of inflammatory-related genes of triploid juvenile rainbow trout (Oncorhynchus mykiss) were investigated. Five diets containing 0.86%, 1.65%, 2.10%, 2.85%, and 3.50% arginine were fed to satiation to triplicate groups of 30 fish per tank for 8 weeks. The results showed that the weight gain rate, specific growth rate, and protein efficiency ratio significantly increased. The optimal dietary arginine requirement was 2.48% (5.97% of dietary protein) based on quadratic regression analysis between specific growth rate and dietary arginine content. There were no significant differences in crude lipid and ash content. Moisture significantly decreased, and crude protein content significantly increased ( P < 0.05 ). Superoxide dismutase, catalase, and lysozyme levels in serum and the liver first significantly increased and then decreased with the level of arginine compared to the control group ( P < 0.05 ), while the malondialdehyde level significantly decreased. Intestinal lipase and amylase levels significantly increased, but there was no significant difference in trypsin level ( P > 0.05 ). The muscular layer thickness and villus width foregut in the 2.85% group were significantly increased. TOR at 2.86% and IGF-1 and S6K1 at 2.11% reached the maximum level, and IL-1β, IL-6, and IL-8 showed a decreasing trend with their lowest levels in the 3.50% and 2.85% groups. The expression of TNF-α first decreased and then increased with the arginine level. Both TGF-β and IL-10 expression first decreased and then increased with the level of arginine and reached the maximum value in the 2.85% group. NF-κB showed an opposite trend and reached the lowest value in the 3.50% group. In conclusion, dietary arginine has a significant effect on growth, serum antioxidant capability, digestion physiology, immunity, digestion, and absorption of nutrients in triploid O. mykiss, and the optimum requirement is 2.48% fed a low-fishmeal diet.

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“…ROS scavenging activity) and singlet oxygen quenchers, as well as assisting in protection of enzymes and photosystem II (Szabados and Savouré, 2010;Rejeb et al, 2014;Çelekli et al, 2016). Conversely, amino acids such as lysine, histidine and arginine have also been shown to have inhibitory effects on algal growth (Dai et al, 2019;Hehmann et al, 2002;Shao et al, 2013). Recent research has J o u r n a l P r e -p r o o f shown arginine can act both as a nutrient source and growth inhibitor of the cyanobacterium Microcystis aeruginosa.…”

Section: Identification Of Compounds Causing Phytotoxicitymentioning

confidence: 99%

“…Previous studies have shown that simple forms of DOM may act as a nutrient source (e.g. Carlsson et al, 1999;Granéli et al, 1999;Dai et al, 2019). The larger molecular weight compounds were historically considered inert or recalcitrant (Thurman et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Identification of compounds from terrestrial dissolved organic matter toxic to cyanobacteria

Neilen

Carroll

Hawker

et al. 2020

Science of The Total Environment

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Effects of arginine on the growth and microcystin-LR production of Microcystis aeruginosa in culture

Cited by 17 publications

References 60 publications

Nitrogen flux into metabolites and microcystins changes in response to different nitrogen sources inMicrocystis aeruginosaNIES‐843

Nitrogen flux into metabolites and microcystins changes in response to different nitrogen sources inMicrocystis aeruginosaNIES‐843

Effects of Dietary Arginine on Growth Performance, Digestion, Absorption Ability, Antioxidant Capability, Gene Expression of Intestinal Protein Synthesis, and Inflammation-Related Genes of Triploid Juvenile Oncorhynchus mykiss Fed a Low-Fishmeal Diet

Identification of compounds from terrestrial dissolved organic matter toxic to cyanobacteria

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