Effects of aqueous garlic extract on oxidant/antioxidant status in 32 D and 32 Dp cell lines

Abstract

Aim:To investigate the possible effects of aqueous garlic extract on the oxidant/antioxidant status and apoptosis in 32D (wild type mouse myeloid cell = normal) and 32Dp210 (BCR-ABL fusion gene (+) mouse myeloid cell = Chronic Myelocytic Leukemia cells) cell lines. Materials and methods:Aqueous garlic extract (10% w/v) was added into the cell line media with 2 different final concentrations (0.4% and 1%). At 0 h and at 24, 48, and 72 h later, the oxidant (malondialdehyde (MDA) level, and xanthine oxidase (XO) enzyme activity) and antioxidant (superoxide dismutase (SOD), glutathione peroxidase (GSHPx), and catalase (CAT) enzyme activities) parameters were measured in the cell lines.Results: It was observed that the garlic extract caused no change in the XO and antioxidant enzyme activities, but it increased the MDA level in the 32D cell line. However, significant increases were found in the MDA level, XO, and antioxidant enzyme activities in the 32Dp210 cell line treated by the garlic extract. Additionally, it was shown that garlic extract had antiproliferative and apoptotic effects on both cell lines. The most effective apoptotic dose was found to be 0.4% (w/v), and at this concentration the death risk of the 32Dp210 cell line was calculated at 2.08 times higher than that of the 32D cell line. Conclusion:It has been suggested that garlic directly causes oxidant stress in the 32D cell line owing to its own oxidant ingredients, and that the oxidant stress created by garlic in the 32Dp210 cell line might occur through increased XO activity and/or its oxidant ingredients. Additionally, antioxidant enzyme activities were found to increase in the 32Dp210 cell line; it would seem that this compensatory change could not prevent the oxidant stress created. We think that the oxidant potential of garlic extract might play a part in its possible anticancer potential, previously supposed by several investigators.

Is garlic a promising food for benign prostatic hyperplasia and prostate cancer?

“…Our findings suggested that oxidant potential of the garlic extract which was created by increased xanthine oxidase activity and/or its own ingredients might play an important role in the anticancer property of garlic. This property of garlic was supposed to be a potential chemo-preventive force in chronic myeloid leukemia [22,23]. In another study, it was concluded that DATS-induced ROS generation in human prostate cancer cells was mediated by an increase in labile iron due to the degradation of ferritin [24].…”

Anti-Proliferation Effects of Garlic (Allium sativum L.) on the Progression of Benign Prostatic Hyperplasia

“…It was also found that urinary frequency and international prostate symptom score values decreased, maximum, and average rates of urine flow increased after the extract consumption. Among the major organosulfur compounds of garlic, diallyl sulfide indicated antioxidant potential against testosterone-induced oxidative stress in prostate of Swiss albino mice (Prasad et al, 2006) and aged garlic extract created oxidant stress, which was reflected by significant increases in malondialdehyde level and xanthine oxidase enzyme activity in 32Dp210 cells (Durak et al, 2006). Considering all of these, garlic and its derivatives have antioxidant properties, which are supposed to affect the improvement of BPH.…”