Effects of aqueous garlic extract on oxidant/antioxidant status in 32D and 32Dp210 cell lines

Methods of Isolation of Active Substances from Garlic (Allium sativum L.) and Its Impact on the Composition and Biological Properties of Garlic Extracts

“…The mechanism of the anticancer activity of bioactive sulfur garlic compounds, such as DAS, DADS, DATS, alliin, SAC, SAMC, and AC, involves alteration of mitochondrial permeability, inhibition of angiogenesis, enhancement of antioxidant and proapoptotic properties, and regulation of cell proliferation [1]. The correlation of the antioxidant potential with the antitumor activity of aqueous garlic extract was also demonstrated by Avci et al [134] in two mouse cell lines, 32D (normal cells) and 32Dp210 (chronic myelocytic leukemia cells) [134]. There was no change in the activity of the xanthine oxidase (XO) enzyme and antioxidant enzymes (SOD, glutathione peroxidase (GSH-Px), CAT), while there was an increase in malondialdehyde (MDA) in the 32D cells.…”

“…In addition, the researchers demonstrated antiproliferative and apoptotic activity in the 32D and 32Dp210 cells. The 0.4% ( w / v ) concentration of the aqueous extract proved to be the most effective, with a 2-fold higher apoptosis rate in the 32Dp210 versus 32D cells [ 134 ]. Szychowski et al described that water extracts of the Polish garlic cultivar “Harnaś” and the Spanish variety “Morado” exhibited strong anticancer properties in the human squamous cancer cell line (SCC-15) measured by ROS production, lactate dehydrogenase (LDH) release, caspase-3 activity, and neutral red uptake methods [ 130 ].…”

“…The anticancer effects of garlic extracts on individual cell lines are presented in Table 4. CACO-2 (human colon carcinoma) Aqueous, methanol, ethanol ROS [130] Crude garlic Inhibition of cell proliferation [137] colo 205 (human colon adenocarcinoma) Crude garlic Reduction in cell viability, induction of apoptosis [138] 32Dp210 (murine myeloid leukemia) Aqueous Oxidant stress [134] HL-60 (human leukemia) Aqueous Cytotoxic effect, apoptosis [139] ALL (precursor-B acute lymphoblastic leukemia) Fresh garlic Selective cells apoptosis [140] U937 (histiocytic lymphoma) Oil ROS, apoptotic [37] TIB (monocyte/macrophage cell line) Crude garlic Inhibition of cell proliferation [137] HepG2 (human hepatoma)…”