Effects of Aqueous Dispersions of C60, C70 and Gd@C82 Fullerenes on Genes Involved in Oxidative Stress and Anti-Inflammatory Pathways

Проскурнина, Е. В.; Mikheev, Ivan V.; Savinova, Ekaterina A.; Ershova, Elizaveta S.; Veiko, Natalia N.; Kameneva, Larisa V.; Долгих, О. А.; Rodionov, Ivan V.; Proskurnin, Mikhail A.; Kostyuk, Svetlana V.

doi:10.3390/ijms22116130

Cited by 5 publications

(14 citation statements)

References 57 publications

(63 reference statements)

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“…Previously [53][54][55][56][57][58][59], we did not observe low-concentration activation of bacterial bioluminescence by the other fullerenols; only high-concentration inhibition (toxic effect) was found. This difference is likely evidence of higher reactivity and reversible electronacceptance ability of Gd@Fln [65,80,81]. Previous experimental and theoretical results support this supposition.…”

Section: Low-concentration Effects Of Gd@flnmentioning

confidence: 74%

Endohedral Gd-Containing Fullerenol: Toxicity, Antioxidant Activity, and Regulation of Reactive Oxygen Species in Cellular and Enzymatic Systems

Sushko

Vnukova

Churilov

et al. 2022

IJMS

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The Gd-containing metallofullerene derivatives are perspective magnetic resonance imaging contrast agents. We studied the bioeffects of a water-soluble fullerene derivative, gadolinium-endohedral fullerenol, with 40–42 oxygen groups (Gd@Fln). Bioluminescent cellular and enzymatic assays were applied to monitor toxicity and antioxidant activity of Gd@Fln in model solutions; bioluminescence was applied as a signaling physiological parameter. The Gd@Fln inhibited bioluminescence at high concentrations (>2·10−1 gL−1), revealing lower toxicity as compared to the previously studied fullerenols. Efficient activation of bioluminescence (up to almost 100%) and consumption of reactive oxygen species (ROS) in bacterial suspension were observed under low-concentration exposure to Gd@Fln (10−3–2·10−1 gL−1). Antioxidant capability of Gd@Fln was studied under conditions of model oxidative stress (i.e., solutions of model organic and inorganic oxidizers); antioxidant coefficients of Gd@Fln were determined at different concentrations and times of exposure. Contents of ROS were evaluated and correlations with toxicity/antioxidant coefficients were determined. The bioeffects of Gd@Fln were explained by hydrophobic interactions, electron affinity, and disturbing of ROS balance in the bioluminescence systems. The results contribute to understanding the molecular mechanism of “hormetic” cellular responses. Advantages of the bioluminescence assays to compare bioeffects of fullerenols based on their structural characteristics were demonstrated.

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Section: Low-concentration Effects Of Gd@flnmentioning

confidence: 74%

Endohedral Gd-Containing Fullerenol: Toxicity, Antioxidant Activity, and Regulation of Reactive Oxygen Species in Cellular and Enzymatic Systems

Sushko

Vnukova

Churilov

et al. 2022

IJMS

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“…The up-to-date concept states that there are extrinsic and intrinsic pathways of apoptosis. The intrinsic apoptosis pathway begins in mitochondria because this process depends on ROS and begins with the peroxidation of cardiolipin under the action of cytochrome C as a catalyst. , We demonstrated that C 60 and C 70 increased the activity of mitochondria after 24 h of incubation . Thus, they can be considered mitochondrial “pro-oxidants”.…”

Section: Discussionmentioning

confidence: 83%

“…The results of flow cytometry confirm the data of fluorescence microscopy. For more images and a more detailed discussion of these results, see our previous article …”

Section: Resultsmentioning

confidence: 89%

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Effects of Aqueous Dispersions of C₆₀, C₇₀, and Gd@C₈₂ Fullerenes on DNA Oxidative Damage/Repair and Apoptosis in Human Embryonic Lung Fibroblasts

Проскурнина

Mikheev

Savinova

et al. 2023

ACS Biomater. Sci. Eng.

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Fullerenes and metallofullerenes play an active role in homeostasis of reactive oxygen species and may cause oxidative damage to cells. As pristine fullerenes are a basis for derivatization, studying oxidative DNA damage/repair and apoptosis is important in terms of genotoxicity and cytotoxicity for their biomedical application. Aqueous dispersions of C 60 , C 70 , and Gd@C 82 (5 nM and 1.5 μM) were cultured with human fetal lung fibroblasts for 1, 3, 24, and 72 h. Oxidative DNA damage/repair was assessed through concentration of 8-oxodG, double-strand breaks, and activation of BRCA1. Activity of apoptosis was assessed through the BCL2/BAX ratio. All three fullerenes caused oxidative modification of DNA at the early stages; C 60 caused the most long-term damage, Gd@C 82 caused the most short-term damage, and C 70 caused "wave-like" dynamics. The dynamics of DNA repair correlated with the dynamics of oxidative damage, but Gd@C 82 caused more prolonged activation of the repair system than C 60 or C 70 . The oxidative toxicity of Gd@C 82 , is minor and the oxidative toxicity of C 60 is mild and short-term, in contrast to C 70 . In relation to the studied effects, the fullerenes can be arranged in a safety row of Gd@C 82 > C 60 > C 70 .

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Structural and optical properties of C₇₀ fullerenes in aqueous solution

Ritter

Nikolenko

Alieksandrov

et al. 2023

Fullerenes, Nanotubes and Carbon Nanostructures

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Effects of Aqueous Dispersions of C60, C70 and Gd@C82 Fullerenes on Genes Involved in Oxidative Stress and Anti-Inflammatory Pathways

Cited by 5 publications

References 57 publications

Endohedral Gd-Containing Fullerenol: Toxicity, Antioxidant Activity, and Regulation of Reactive Oxygen Species in Cellular and Enzymatic Systems

Endohedral Gd-Containing Fullerenol: Toxicity, Antioxidant Activity, and Regulation of Reactive Oxygen Species in Cellular and Enzymatic Systems

Effects of Aqueous Dispersions of C₆₀, C₇₀, and Gd@C₈₂ Fullerenes on DNA Oxidative Damage/Repair and Apoptosis in Human Embryonic Lung Fibroblasts

Structural and optical properties of C₇₀ fullerenes in aqueous solution

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Effects of Aqueous Dispersions of C60, C70 and Gd@C82 Fullerenes on Genes Involved in Oxidative Stress and Anti-Inflammatory Pathways

Cited by 5 publications

References 57 publications

Endohedral Gd-Containing Fullerenol: Toxicity, Antioxidant Activity, and Regulation of Reactive Oxygen Species in Cellular and Enzymatic Systems

Endohedral Gd-Containing Fullerenol: Toxicity, Antioxidant Activity, and Regulation of Reactive Oxygen Species in Cellular and Enzymatic Systems

Effects of Aqueous Dispersions of C60, C70, and Gd@C82 Fullerenes on DNA Oxidative Damage/Repair and Apoptosis in Human Embryonic Lung Fibroblasts

Structural and optical properties of C70 fullerenes in aqueous solution

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Effects of Aqueous Dispersions of C₆₀, C₇₀, and Gd@C₈₂ Fullerenes on DNA Oxidative Damage/Repair and Apoptosis in Human Embryonic Lung Fibroblasts

Structural and optical properties of C₇₀ fullerenes in aqueous solution