“…Adult peripheral nervous system (PNS) tissue has been successfully cultured and shown to have regenerative capacity by several workers (adult human sympathetic ganglia, Murray and Stout, 1974; adult rat sympathetic ganglia, Silberstein, Johnson, Hanbuaser, Bloom, and Kopin, 1971; adult frog sympathetic ganglia, Hill and Burnstock, 1975; adult frog dorsal root ganglia, Padjen, Forman, and Siggins, 1975). Most attempts to culture adult mammalian central nervous system (CNS) tissue met with less success (human cortex, Costero and Pomerat, 1951;Hogue, 1953;Geiger, 1958; individual vestibular neurons of the rabbit, Hillman and Sheikh, 1968; rat spinal cord and cerebellum, Kiernan and Pettit, 1971;Drayton and Kiernan, 1973; dissociated rat hypothalamus, Wilkinson, Gibson, Bressler, and Inman, 1974). Greater success was achieved recently by De Boni, Seger, Scott, and Crapper, (1976) who described regeneration of nerve fibers and neurotypic electrical and synaptic activity in cultures of adult teleost CNS maintained for periods of up to 19 weeks.…”