Effects of aprotinin on organ cultures of the cerebellum of the adult rat

Drayton, M.R.; Kiernan, J. A.

doi:10.1016/0014-4886(73)90024-1

Cited by 11 publications

(2 citation statements)

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“…Adult peripheral nervous system (PNS) tissue has been successfully cultured and shown to have regenerative capacity by several workers (adult human sympathetic ganglia, Murray and Stout, 1974; adult rat sympathetic ganglia, Silberstein, Johnson, Hanbuaser, Bloom, and Kopin, 1971; adult frog sympathetic ganglia, Hill and Burnstock, 1975; adult frog dorsal root ganglia, Padjen, Forman, and Siggins, 1975). Most attempts to culture adult mammalian central nervous system (CNS) tissue met with less success (human cortex, Costero and Pomerat, 1951;Hogue, 1953;Geiger, 1958; individual vestibular neurons of the rabbit, Hillman and Sheikh, 1968; rat spinal cord and cerebellum, Kiernan and Pettit, 1971;Drayton and Kiernan, 1973; dissociated rat hypothalamus, Wilkinson, Gibson, Bressler, and Inman, 1974). Greater success was achieved recently by De Boni, Seger, Scott, and Crapper, (1976) who described regeneration of nerve fibers and neurotypic electrical and synaptic activity in cultures of adult teleost CNS maintained for periods of up to 19 weeks.…”

Section: Introductionmentioning

confidence: 99%

Adult mouse dorsal root ganglia neurons in cell culture

Scott

1977

J. Neurobiol.

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A method has been developed for the long-term culture of dissociated adult mouse dorsal root ganglia (DRG). Of critical importance to the success of this technique was a three-hour incubation in collagenase which softened the DRG and permitted gentle dissociation. The morphological and electrophysiological features of the dissociated adult DRG were similar to those observed in previous studies of immature (i.e., embryonic and newborn) DRG in culture and also to those of adult DRG in situ. With regard to electrophysiological work, the adult DRG neurons are superior to embryonic and newborn neurons because of their larger size and greatly increased survival in culture (no degeneration for first six days, and thereafter a relatively slow decrease). The adult neurons regenerated nerve fibers to an extent comparable to that of immature neurons. Therefore, the adult DRG cultures might be useful to study factors influencing regeneration in the adult mammalian nervous system. The adult cultures might also be useful to investigated factors influencing the aging process.

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Section: Introductionmentioning

confidence: 99%

Adult mouse dorsal root ganglia neurons in cell culture

Scott

1977

J. Neurobiol.

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“… does not survive autotransplantation. Small pieces of adult central nervous tissue can be maintained in culture for up to 2 weeks (Kiernan & Pettit, 1971 ; Tsiquaye & Zuckerman, 1974), though many of their cells die(Drayton & Kiernan, 1973; …”

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confidence: 99%