Effects of application rates on maleic hydrazide residues in Burley tobacco

Haeberer, Alfred F.; Nichols, B. C.; Chortyk, O. T.

doi:10.1021/jf60216a016

Cited by 5 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hydrazine is then steam distilled into an acidic p-dimethylaminobenzaldehyde solution and the resulting yellow compound has an absorption maximum at 355 nm (3-5). ss Some authors determine the maleic hydrazide in tobacco and in tobacco smoke by gas chromatographic procedures, using methyl or trimethylsilyl or alkyl carbonate derivatives (6)(7)(8)(9)(10)(11)(12)(13). Others describe HPLC methods involving purifications by passages through several different resins (14)(15)(16)(17).…”

Section: Discussionmentioning

confidence: 99%

Determination of Free and Bound Maleic Hydrazide Residues in Tobacco by High Performance Liquid Chromatography

Dattilo¹,

Gallo²,

Lionetti³

et al. 1994

Beiträge Zur Tabakforschung International/Contributions to Tobacco Research

View full text Add to dashboard Cite

A new method is described for the qualitative and quantitative determination of both free and bound maleic hydrazide residues in tobacco leaves and cigarette filler by high performance liquid chromatography. Analyses were carried out by hydrolyzing samples of ground tobacco with 4 N hydrochloric acid for 40 minutes under reflux followed by sample chromatography, running isocratic elutions with a dilute solution of phosphoric acid. The quantitative determination of maleic hydrazide was performed by light absorption at 320 nm, by the calibration curve method. Recoveries of maleic hydrazide added to tobacco samples were greater than 90 %. The detection limit of the method, determined on ground tobacco leaves, was at least 5 ppm. The results obtained by this procedure and by the ISO standard method no. 4876 are in good accordance.

show abstract