Effects of application of sucrose and cytokinin to roots on the formation of tuberous roots in sweetpotato (Ipomoea batatas (L.) Lam.)

Eguchi, Tetsuo; Yoshida, Satoshi

doi:10.3117/plantroot.2.7

Cited by 33 publications

(30 citation statements)

References 25 publications

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“…Our results suggest that at I-stage and in swollen root, phytohormones act in concert to regulate phase transition and primary thickening growth by inducing secondary cambium and metaxylem differentiation and nutrient mobilization (Fig. 4) (Eguchi and Yoshida 2008;Ioio et al 2008;Noh et al 2010;Overvoorde et al 2010;Perrot-Rechenmann 2010;Roumeliotis et al 2012b;Wang et al 2006). …”

Section: Role Of Cytokinin and Auxin In Storage Root Initiationmentioning

confidence: 88%

“…Also the overexpression of CK in potato could not induce tuberization in all events (Sergeeva et al 2000). The combination of auxin and CK induced early tuber initiation and inhibited stolon elongation by counteracting the GA function, while CK and sucrose (but not auxin) induced secondary thickening growth of the tuber (Eguchi and Yoshida 2008;Wang et al 2006;Xu et al 1998). In I-stage, AUX1, PIN1 and KS were downregulated but CRF1 and ARR3 were upregulated.…”

Section: Role Of Cytokinin and Auxin In Storage Root Initiationmentioning

confidence: 96%

“…Low levels of GA and auxin, together with high levels of cytokinin (CK), are involved in storage organ formation and enhancement of enzyme activities in the starch biosynthesis pathway (Ravi et al 2014;Sergeeva et al 2000). In sweet potato, whose storage organ originates from root as in cassava, CK, GA, ethylene and auxin have also been found to influence SR development (Eguchi and Yoshida 2008;Ku et al 2008;McGregor 2006;Noh et al 2010;Wang et al 2006).…”

Section: Introductionmentioning

confidence: 97%

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Genome-wide analysis reveals phytohormone action during cassava storage root initiation

et al. 2015

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Development of storage roots is a process associated with a phase change from cell division and elongation to radial growth and accumulation of massive amounts of reserve substances such as starch. Knowledge of the regulation of cassava storage root formation has accumulated over time; however, gene regulation during the initiation and early stage of storage root development is still poorly understood. In this study, transcription profiling of fibrous, intermediate and storage roots at eight weeks old were investigated using a 60-mer-oligo microarray. Transcription and gene expression were found to be the key regulating processes during the transition stage from fibrous to intermediate roots, while homeostasis and signal transduction influenced regulation from intermediate roots to storage roots. Clustering analysis of significant genes and transcription factors (TF) indicated that a number of phytohormone-related TF were differentially expressed; therefore, phytohormone-related genes were assembled into a network of correlative nodes. We propose a model showing the relationship between KNOX1 and phytohormones during storage root initiation. Exogeneous treatment of phytohormones N (6) -benzylaminopurine and 1-Naphthaleneacetic acid were used to induce the storage root initiation stage and to investigate expression patterns of the genes involved in storage root initiation. The results support the hypothesis that phytohormones are acting in concert to regulate the onset of cassava storage root development. Moreover, MeAGL20 is a factor that might play an important role at the onset of storage root initiation when the root tip becomes swollen.

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Section: Role Of Cytokinin and Auxin In Storage Root Initiationmentioning

confidence: 88%

Section: Role Of Cytokinin and Auxin In Storage Root Initiationmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 97%

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Genome-wide analysis reveals phytohormone action during cassava storage root initiation

et al. 2015

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“…In sweetpotato, endogenous levels of cytokinin and auxin rapidly increase in the roots during tuberous root development (Nakatani & Komeichi, 1991). Exogenous application of cytokinin also activates tuberous root formation (McDavid & Alamu, 1980;Eguchi & Yoshida, 2008). Given that SRD1, a gene which activates the development of storage roots, is highly induced by exogenous auxin treatment (Noh et al, 2010), manipulations of root hormonal levels using pharmacological treatments or environmental regulation may be employed to increase the tuberous root yield.…”

Section: Resultsmentioning

confidence: 99%

Effect of Pot Volume on the Growth of Sweetpotato Cultivated in the New Hydroponic System

Sakamoto¹,

Suzuki²

2018

SAR

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Hydroponics is an effective means for promoting plant growth as it facilitates water and nutrient uptake by plant roots. For increasing the production of sweetpotato (Ipomoea batatas), we developed the new hydroponic cultivation system in which tuberous roots were grown in solid media in the pots whereas fibrous roots were grown in the nutrient solution. Using this method, the effect of pot volume (1.6, 3.0, and 4.5 L) on the growth of sweetpotato was investigated. When plants were grown in small-sized pots (1.6 L), the fresh weight of the top and that of tuberous roots were decreased compared with plants grown in 3.0 L and 4.5 L pots. No clear difference was observed between the top and the tuberous roots in terms of the dry weight ratio, regardless of the pot size. The number of tuberous roots per plant and the maximum tuberous root weight were not influenced by the pot size either. However, the number of tuberous roots weighing more than 100 g was decreased in plants grown in small pots. Some of the tuberous roots grown in this hydroponic system contained a non-hypertrophic parts with severely lignified metaxylems. These results suggest that the environment surrounding the tuberous root influenced by the pot volume may be important for root enlargement in this hydroponic system.

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“…Furthermore, the exogenous applications of synthetic cytokinins were reported to be effective in promoting storage root formation (Spence and Humphries, 1972;McDavid and Alamu, 1980;Nakatani 1994). The exogenous application of cytokinin induced storage root formation in the presence of high sucrose concentrations (Eguchi and Yoshida, 2008). Cytokinins are prerequirement for formation and proliferation of the cambial cell files in roots and stem (Miho et al 2008;Nieminen et al 2008).…”

Section: A Cytokininsmentioning

confidence: 99%

Molecular Regulation of Storage Root Formation and Development in Sweet Potato

Ravi¹,

Chakrabarti²,

Makeshkumar³

et al. 2014

Horticultural Reviews: Volume 42

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Storage root formation and development in sweet potato [Ipomoea batatas (L) Lam. Convolvulaceae] is a complex process characterized by the cessation of root elongation, genesis of vascular cambium, anomalous and interstitial cambia, and increase in radial growth by increased cell proliferation and expansion concomitant with the massive deposition of starch and storage proteins that eventually result in storage root enlargement. Phytohormones play a crucial role in the formation of storage roots. Three class I knotted-like homeobox (KNOX1) genes-SRF1, SRF5, and SRF6-modulate carbohydrate metabolism and cell division. The genes Ibkn1 and Ibkn2 activate cytokinin biosynthesis. Transcription factors derived from MADS box genes IbMADS1, IbMADS3, IbMADS4, and IbAGL17 induce signal transduction pathway leading to storage root formation and development. The occurrence of SRD1 transcripts mainly in the actively dividing cells, including the vascular and cambium cells, and the increase in endogenous indole-3-acetic acid (IAA) content and three auxin-inducible AUX/IAA gene transcripts concomitantly with SRD1 transcripts suggest the involvement of SRD1 during the early stage of storage root development. Along with IbMADS1 induction, two storage root marker genes, which encode a major storage protein sporamin and IbAGPase that encodes AGPase for ADP-glucose production in starch biosynthesis, are up-regulated during the early period of storage root development. A class III HD-Zip protein 8 regulating the development of cambia and secondary vascular tissues, a short-root protein that is a key regulator in root 157 radial patterning, meristem maintenance, and asymmetric cell division, the expansin gene IbEXP1 encoding a cell wall loosening protein, genes encoding cyclin A-and cyclin D-like proteins, and five cyclin-dependent kinases are upregulated during storage root formation. Genes encoding ADP-glucose pyrophosphorylase, granule-bound starch synthase, starch synthase, and phosphoglucomutase are up-regulated, whereas genes encoding pyruvate decarboxylase and lactate dehydrogenase are down-regulated during storage root development. The endogenous sucrose levels influence the expression of two AGPase isoforms: ibAGP1 and ibAGP2. The expression of cell wall-bound (extracellular/apoplast) acid invertase activity gene (cwINV) predominates during early period, whereas the expression of cytosolic activity of sucrose synthase gene (SuSy) predominates during later period of storage root enlargement. The competition between lignification and formation of anomalous cambia and the associated starchaccumulating cells determine storage root development. Genes encoding enzymes such as coumaroyl-CoA synthase, caffeoyl-CoA O-methyltransferase, and cinnamyl alcohol dehydrogenase during storage root formation reduce lignification in tissue sections of storage roots.

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Effects of application of sucrose and cytokinin to roots on the formation of tuberous roots in sweetpotato (Ipomoea batatas (L.) Lam.)

Cited by 33 publications

References 25 publications

Genome-wide analysis reveals phytohormone action during cassava storage root initiation

Genome-wide analysis reveals phytohormone action during cassava storage root initiation

Effect of Pot Volume on the Growth of Sweetpotato Cultivated in the New Hydroponic System

Molecular Regulation of Storage Root Formation and Development in Sweet Potato

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