Effects of antioxidants on motility and membrane integrity of chilled-stored stallion semen

Aurich, Jörg; Schönherr, U.; Hoppe, Heinrich C.; Aurich, Christine

doi:10.1016/s0093-691x(97)84066-6

Cited by 172 publications

(111 citation statements)

References 17 publications

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“…Lipid peroxidation usually results in irreversible loss of sperm motility and DNA damage (De Lamirande and Gagnon, 1992;Aitken, 1994;Maxwell and Watson, 1996); Ruminant semen normally contains antioxidants, including taurine, hypotaurine, Catalase (CAT) and reduced glutathione (GSH), which can offset lipid peroxidation (Jeulin et al, 1989;Griveau et al, 1995;Kantola et al, 1998). However, this endogenous antioxidative capacity may be insufficient to prevent lipid peroxidation during prolonged storage (Aurich et al, 1997). Thus the addition of external antioxidants such as melatonin might protect the sperm cells against the harmful effects of these oxidative stressors (ROS) and subsequently improve sperm motility during sperm storage (Ashrafi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Evidences for the Role of Melatonin as a Protective Additive During Buffalo Semen Freezing

Raey¹,

Badr²,

Rawash³

et al. 2014

American Journal of Animal and Veterinary Sciences

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Till now, the exact role of melatonin in male infertility hasn't been fully discovered. Moreover, the intracellular signaling pathways activated by melatonin in buffalo spermatozoa especially on the level of cryopreservation process haven't been reported. The current study aimed simply to clarify the precise role of this hormone during buffalo semen cryopreservation. Semen samples were obtained randomly from 6 fertile buffalo bulls (aged 3 to 5 years). Weekly, two consecutive ejaculates were collected from each bull for successive six weeks duration using an artificial vagina. The ejaculates were pooled to eliminate variability between the evaluated samples. After that, semen samples were subjected to extension with Tris-based extender supplemented with different concentrations of melatonin (0.1, 0.25, 0.5, 0.75 and 1 mM) Vs. Trisbased extender only (control). Then they were processed to cryopreservation and thawing to assess the different semen characteristics. It had been found that 0.1 mM of melatonin significantly (p<0.05) improved post thawing motility, viability index, acrosomal integrity, total antioxidant capacity, superoxide dismutase and glutathione reductase activity. On the other hand, it significantly (p<0.05) decreased the rate of lipid peroxidation, aspartate amino transferase, alanine aminotransferase, alkaline phosphatase and DNA fragmentation. All of these previously enhanced semen characteristics were reflected positively on its in vitro fertilizing capacity, as well as the percent of harvested embryos. In conclusion melatonin supplementation to the extension media of buffalo semen during processing significantly enhanced its characteristics. Moreover, the preceding results focusing more light on the potential roles of melatonin in regulating male reproduction.

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Section: Introductionmentioning

confidence: 99%

Evidences for the Role of Melatonin as a Protective Additive During Buffalo Semen Freezing

Raey¹,

Badr²,

Rawash³

et al. 2014

American Journal of Animal and Veterinary Sciences

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“…Although ROS can adversely affect sperm function (Aitken, 1995), low levels appear to be a prerequisite for normal sperm functioning, being involved in cell signalling and fertilization. However, in situations where spermatozoa are stored before AI, they may be damaged by rising levels of ROS in the environment (Aurich et al, 1997). Some ROSs are known to have a negative effect on sperm membrane integrity (Alvarez et al, 1987;Griveau et al, 1995), with excessive levels causing damage to sperm DNA and RNA, possibly initiating apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Naturally and stimulated levels of reactive oxygen species in cooled stallion semen destined for artificial insemination

Johannisson

Lundgren

Humblot

et al. 2014

Animal

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The decrease in foaling rates after artificial insemination with cooled semen warrants the search for new predictors of fertility. The objectives were to investigate levels of naturally occurring reactive oxygen species (ROS) in cooled, stored stallion semen doses for artificial insemination (AI), and their relationship with parameters of semen quality and with pregnancy rate. Semen was collected from warmblood stallions (n = 15) and used to prepare commercial semen doses for AI. Sperm quality was evaluated after cooled transport to the laboratory overnight. The results were correlated with observed foaling and pregnancy rates. Hydroethidine and dichlorodihydrofluorescein diacetate were used as indicators for the ROS superoxide and hydrogen pero xide, respectively. Sperm morphology, motility, plasma membrane integrity and chromatin integrity were also evaluated. These variables were correlated with each other and with pregnancy rates. We found a high inter-individual variation in the ROS levels between stallions. The proportion of live, hydrogen peroxide-negative spermatozoa was correlated with progressive motility, whereas live hydrogen peroxide-negative spermatozoa and chromatin damage were negatively correlated, indicating that low levels of hydrogen peroxide were correlated with good chromatin integrity. The percentage of dead hydrogen peroxide-positive sperm was negatively related to the foaling rate. The negative relationships were stronger when combining results from both assays for ROS. These results for stored semen samples indicate that high individual variation exists for superoxide and hydrogen peroxide measurements, and that ROS status can influence sperm quality. Thus, ROS may be some of the factors influencing fertility. Moreover, combinations of ROS variables improved the correlation with fertility, indicating the usefulness of including these variables in a future model for prediction of the fertility of a semen sample.

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“…Adding hyaluronic acid (1000 µg/ml) to the freezing extender enhanced post-thaw motility of boar and human sperm (11,12). Contradictory reports existing on the effect of antioxidant supplementation on postthaw motility of semen from various species, including the buck (4,8,17,24,25), were resulted from the different the types and doses of the antioxidants employed. These equivocal results may be related not only to the different degrees of susceptibility of sperm cells (among the species) to lipid peroxidation, but also due to the differences in the contents of antioxidants and/or the composition of seminal plasma concerned.…”

Section: Discussionmentioning

confidence: 99%

In vitro evaluation of Saanen buck semen frozen in different extenders supplemented with various antioxidants

Recai;DAŞKIN¹

2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aim of this present study was to investigate the effects of extenders containing different antioxidants upon the principle sperm characteristics of Saanen buck semen following freezing. In this study, four bucks were used. Semen was collected twice a week by an artificial vagina during the breeding season to obtain ten ejaculates per animal. The samples were then extended in three extenders (tris, T-, skimmed milk, M-and laiciphos 488, L-based) without (as controls) or with the two antioxidants (5 mM cysteine, C and 1000 µg/ml hyaluronic acid, H). By this way, nine experimental groups were assigned, as follows:For the T extender groups, the rates of motility and viability were significantly (p<0.01) higher for the T-C extender as compared to those of the T-H and T-O. However, there was no such difference (p>0.05) among the extenders based on the rates of abnormal spermatozoa, abnormal acrosome and hypoosmotic swelling (HOS) test. For the M groups, the motility was significantly (p<0.01) higher for the M-C and M-O extenders as compared to that of the M-H. However, there was no such difference (p>0.05) among the extenders based on the rates of viability, abnormal spermatozoa, abnormal acrosome and HOS test. For the L groups, the rates of motility and viability were significantly (p<0.01) higher for the L-C extender as compared to that of the L-H but not that of the L-O. Additionally, there was no such difference (p>0.05) among the extenders mentioned above based on the rates of abnormal spermatozoa, abnormal acrosome and HOS test. As a result, the present in vitro findings of semen quality parameters studied suggest that; i) both skimmed milk and laiciphos extenders were more favourable than tris, and that ii) the cysteine, as an antioxidant, provided a superior protection than the hyaluronic acid did.

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Effects of antioxidants on motility and membrane integrity of chilled-stored stallion semen

Cited by 172 publications

References 17 publications

Evidences for the Role of Melatonin as a Protective Additive During Buffalo Semen Freezing

Evidences for the Role of Melatonin as a Protective Additive During Buffalo Semen Freezing

Naturally and stimulated levels of reactive oxygen species in cooled stallion semen destined for artificial insemination

In vitro evaluation of Saanen buck semen frozen in different extenders supplemented with various antioxidants

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