Effects of Antihypertensive Drugs on Angiotensinase Activities in the Testis of Spontaneously Hypertensive Rats

Segarra, Ana Belén; Prieto, Isabel; Villarejo, Ana Belén; Banegas, Inmaculada; Wangensteen, Rosemary; Gasparo, Marc de; Vives, Francisco; Ramírez-Sánchez, Manuel

doi:10.1055/s-0032-1329988

Cited by 5 publications

(6 citation statements)

References 20 publications

(25 reference statements)

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“…However, propranolol at the dose used did not modify SBP either in hypertensive animals as previously reported [13] or in WKY rats (fig. 2).…”

Section: Discussionsupporting

confidence: 80%

“…However, SBP was unaffected after propranolol treatment either in WKY or in SHR [13]. SBP was significantly higher in the control (p < 0.001), L -NAME- (p < 0.05) and propranolol- (p < 0.001) treated SHR groups than in the WKY group (fig.…”

Section: Resultsmentioning

confidence: 99%

“…All of the experimental procedures involving animals were performed in accordance with the European Communities Council Directive 86/609/EEC and were approved by the bioethics committee of the University of Jaén. L -NAME (Sigma; 70 mg/kg/day) and propranolol (Sigma; 100 mg/kg/day) were given in drinking water for 4 weeks [4,13]. Chronic administration of L -NAME and propranolol in drinking water at these dosages has been reported to be an appropriate method to achieve the full effect of the drugs [10,14].…”

Section: Methodsmentioning

confidence: 99%

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Brain, Heart and Kidney Correlate for the Control of Blood Pressure and Water Balance: Role of Angiotensinases

Prieto¹,

Villarejo²,

Segarra³

et al. 2014

Neuroendocrinology

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The renin-angiotensin system (RAS) plays a major role in the control of blood pressure (BP) and water balance by coordinating brain, heart and kidney functions, connected with each other by hormonal and neural mechanisms through the autonomic nervous system (ANS). RAS function may be monitored by the study of the enzymes (angiotensinases) involved in the metabolism of its active peptides. In order to study the relationship between the brain-heart-kidney axis and the control of BP and water balance, we analyzed the correlation of angiotensinase activities, assayed as arylamidase activities, between hypothalamus, left ventricle, renal cortex and renal medulla, collected from Wistar-Kyoto and spontaneously hypertensive rats, treated or not treated with L-NAME [N(G)-nitro-L-arginine methyl ester]. This compound not only inhibits the formation of nitric oxide but also disrupts the normal function of the ANS activating the sympathetic nervous system (SNS) to increase BP. In addition, to assess the influence of the SNS, we studied the effect of its blockade by treatment of both strains with propranolol. The present results support the notion that RAS function of the brain-heart-kidney axis, as reflected by the activities of angiotensinases, is reciprocally connected by afferent and efferent mechanisms between these locations, presumably through the ANS. These results reveal new aspects of neuroendocrine regulation possibly involving the ANS.

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“…However, propranolol at the dose used did not modify SBP either in hypertensive animals as previously reported [13] or in WKY rats (fig. 2).…”

Section: Discussionsupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Brain, Heart and Kidney Correlate for the Control of Blood Pressure and Water Balance: Role of Angiotensinases

Prieto¹,

Villarejo²,

Segarra³

et al. 2014

Neuroendocrinology

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show abstract

“…A local renin-angiotensin system (RAS) has been clearly established in testis which may participate in male reproductive functions [ 9 , 10 , 11 ]. It has been suggested that Ang II-metabolizing activity (glutamyl aminopeptidase, GluAP) may be influenced by dietary fatty acids composition and cholesterol content during early mice development [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of a Virgin Olive Oil versus Butter Plus Cholesterol-Enriched Diet on Testicular Enzymatic Activities in Adult Male Rats

Domínguez-Vías

Segarra

Martı́nez-Cañamero

et al. 2017

IJMS

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The aim of the present work was to improve our knowledge on the mechanisms underlying the beneficial or deleterious effects on testicular function of the so-called Mediterranean and Western diet by analyzing glutamyl aminopeptidase (GluAP), gamma glutamyl transpeptidase (GGT) and dipeptidyl peptidase IV (DPP IV) activities in testis, as enzymes involved in testicular function. Male Wistar rats (6 months old) were fed for 24 weeks with three different diets: standard (S), an S diet supplemented with virgin-olive-oil (20%) (VOO), or a S diet enriched with butter (20%) plus cholesterol (0.1%) (Bch). At the end of the experimental period, plasma lipid profiled (total triglycerides, total cholesterol and cholesterol fractions (HDL, LDL and VDL)) were measured. Enzymatic activities were determined by fluorimetric methods in soluble (sol) and membrane-bound (mb) fractions of testicular tissue using arylamide derivatives as substrates. Results indicated an increase in plasmatic triglycerides, total cholesterol, LDL and VLDL in Bch. A significant increase of mb GluAP and GGT activities was also found in this diet in comparison with the other two diets. Furthermore, significant and positive correlations were established between these activities and plasma triglycerides and/or total cholesterol. These results support a role for testicular GluAP and GGT activities in the effects of saturated fat (Western diet) on testicular functions. In contrast, VOO increased sol DPP IV activity in comparison with the other two diets, which support a role for this activity in the effects of monounsaturated fat (Mediterranean diet) on testicular function. The present results strongly support the influence of fatty acids and cholesterol on testicular GluAP and GGT activities and also provide support that the reported beneficial influence of the Mediterranean diet in male fertility may be mediated in part by an increase of testicular sol DPP IV activity.

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“…These alterations, depending on the degree of fatty acid saturation, are related to changes in the systemic or local renin-angiotensin systems (RAS) [5][6][7][8][9][10]. The activation of the RAS participates in the development of MetS, heart failure [11], and pathophysiology of hypertension [4,7,[12][13][14][15][16][17][18][19][20][21][22][23][24][25]. Within these local RAS, the type of fatty acids consumed with the diet allows modifying various enzymes of the aminopeptidase We hypothesized that the inclusion of MUFA in the diet could be a preventive dietary factor for reducing the activation of the RAS pathway through angiotensinases and maintaining the functional integrity of cardiovascular structures.…”

Section: Introductionmentioning

confidence: 99%

High-Fat Diets Modify the Proteolytic Activities of Dipeptidyl-Peptidase IV and the Regulatory Enzymes of the Renin–Angiotensin System in Cardiovascular Tissues of Adult Wistar Rats

et al. 2021

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(1) Background: The replacement of diets high in saturated fat (SAFA) with monounsaturated fatty acids (MUFA) is associated with better cardiovascular function and is related to the modulation of the activity of the local renin–angiotensin system (RAS) and the collagenase activity of dipeptidyl peptidase IV (DPP-IV). The objective of the work was to verify the capacity of different types of dietary fat on the regulatory activities of RAS and DPP-IV. (2) Methods: Male Wistar rats were fed for 24 weeks with three different diets: the standard diet (S), the standard diet supplemented with virgin olive oil (20%) (VOO), or with butter (20%) plus cholesterol (0.1%) (Bch). The proteolytic activities were determined by fluorometric methods in the soluble (sol) and membrane-bound (mb) fractions of the left ventricle and atrium, aorta, and plasma samples. (3) Results: With the VOO diet, angiotensinase values were significantly lower than with the Bch diet in the aorta (GluAP and ArgAP (mb)), ventricle (ArgAP (mb)) and atrium (CysAP (sol)). Significant decreases in DPP-IV (mb) activity occurred with the Bch diet in the atrium and aorta. The VOO diet significantly reduced the activity of the cardiac damage marker LeuAP (mb) in the ventricle and aorta, except for LeuAP (sol) in the ventricle, which was reduced with the Bch diet. (4) Conclusions: The introduction into the diet of a source rich in MUFA would have a beneficial cardiovascular effect on RAS homeostasis and cardiovascular functional stability.

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Effects of Antihypertensive Drugs on Angiotensinase Activities in the Testis of Spontaneously Hypertensive Rats

Cited by 5 publications

References 20 publications

Brain, Heart and Kidney Correlate for the Control of Blood Pressure and Water Balance: Role of Angiotensinases

Brain, Heart and Kidney Correlate for the Control of Blood Pressure and Water Balance: Role of Angiotensinases

Influence of a Virgin Olive Oil versus Butter Plus Cholesterol-Enriched Diet on Testicular Enzymatic Activities in Adult Male Rats

High-Fat Diets Modify the Proteolytic Activities of Dipeptidyl-Peptidase IV and the Regulatory Enzymes of the Renin–Angiotensin System in Cardiovascular Tissues of Adult Wistar Rats

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