Effects of Antifungal Soaked Silicone Hydrogel Contact Lenses on Candida albicans in an Agar Eye Model

Phan, Chau-Minh; Bajgrowicz, Magdalena; McCanna, David J.; Subbaraman, Lakshman N.; Jones, Lyndon

doi:10.1097/icl.0000000000000209

Cited by 11 publications

(5 citation statements)

References 33 publications

(46 reference statements)

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“…22,27–29 The setup of the eye model with attachment to a microfluidic system is shown in Figure 1. The humidity was maintained using a humidifier and hygrometer to a minimum of 50% air moisture.…”

Section: Methodsmentioning

confidence: 99%

Release of Moxifloxacin from Contact Lenses Using an In Vitro Eye Model: Impact of Artificial Tear Fluid Composition and Mechanical Rubbing

Phan

Bajgrowicz-Cieslak

Subbaraman

et al. 2016

Trans. Vis. Sci. Tech.

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PurposeThe aim of this study was to evaluate and compare the release of moxifloxacin from a variety of daily disposable (DD) contact lenses (CLs) under various conditions using a novel in vitro eye model.MethodsFour commercially available DD conventional hydrogel (CH) CLs (nelfilcon A, omafilcon A, etafilcon A, and ocufilcon B) and three silicone hydrogel (SH) CLs (somofilcon A, narafilcon A, and delefilcon A) were evaluated. These lenses were incubated in moxifloxacin for 24 hours. The release of the drug was measured using a novel in vitro model in three experimental conditions: (1) phosphate buffered saline (PBS); (2) artificial tear solution (ATS) containing a variety of proteins and lipids; and (3) ATS with mechanical rubbing produced by the device.ResultsOverall, CH CLs had a higher drug release than SH CLs (P < 0.05) under all conditions. Typically, a higher drug release was observed in PBS than ATS (P < 0.05). For CH, drug release was found to be higher in ATS with rubbing than PBS or ATS (P < 0.05). For most lens types, ATS with rubbing produced higher drug release than ATS alone (P < 0.05). Generally, the release kinetics for all conditions were sustained over the 24-hour testing period, and no burst release was observed (P < 0.05).ConclusionsMoxifloxacin release from a CL into ATS is lower when compared to release into PBS. When mechanical rubbing is introduced, the amount of drugs released is increased.Translational RelevanceResults suggest that sophisticated in vitro models are necessary to adequately model on-eye drug release from CL materials.

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Section: Methodsmentioning

confidence: 99%

Release of Moxifloxacin from Contact Lenses Using an In Vitro Eye Model: Impact of Artificial Tear Fluid Composition and Mechanical Rubbing

Phan

Bajgrowicz-Cieslak

Subbaraman

et al. 2016

Trans. Vis. Sci. Tech.

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“…For the cornea, the hydrogel does not need to be flexible or as durable, which allows for more material selections. In the past, our laboratory used agar as a polymer for the eyeball to evaluate the growth of microbes on a model eye 63. Gelatin, collagen, or their derivatives could potentially be used as a material for the eyeball to grow an overlying layer of corneal epithelial cells to better mimic the ocular surface 64…”

Section: Discussionmentioning

confidence: 99%

Development of an Eye Model With a Physiological Blink Mechanism

Phan

Walther

Qiao

et al. 2019

Trans. Vis. Sci. Tech.

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Purpose To develop an eye model with a physiological blink mechanism. Methods All parts of the eye model were designed using computer-aided design software. The eyelid consisted of a unique 3D printed structure containing teeth to physically secure a flexible membrane. Both the eyeball and eyelid membrane were synthesized using polyvinyl alcohol (PVA). Four molecular weights of PVA (89–98, 85–124, 130, and 146–186 kDa) were tested at a range of concentrations between 5% and 30% weight/volume. The wettability and water content of these materials were compared with the bovine cornea and sclera. The model was connected to a microfluidic pump, which delivers artificial tear solution (ATS) to the eyelid. A corneal topographer was used to evaluate the tear break-up and tear film regeneration. Results The eyelid flexes and slides across the eyeball during each blink, which ensures direct contact between the two surfaces. When loaded with an ATS, this mechanism evenly spreads the solution over the eyeball to generate an artificial tear film. The artificial tear film in this eye model had a tear break-up time (TBUT) of 5.13 ± 0.09 seconds at 1.4 μL/min flow rate, 6 blinks/min, and <25% humidity. Conclusions This model simulates a physiological blink actuation and an artificial tear film layer. Future studies will examine variations in flow rates and ATS composition to simulate clinical values of TBUT. Translational Relevance The eye model could be used to study in vitro TBUT, tear deposition, and simple drug delivery.

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“…Substantial research was conducted on the use of lenses as a drug carrier for ocular delivery. For example, they were investigated for many drugs such as ciprofloxacin [48], cyclosporine [49], dexamethasone [50], timolol [51], antifungal drugs [52], among others. Drug-eluting lenses were reported to increase drugs ocular bioavailability via prolongation of their duration of action and increase their corneal penetration [47].…”

Section: New and Advanced Ocular Drug Delivery Systems 61 Contact Lensesmentioning

confidence: 99%

Recent Strategies for Ocular Drug Delivery: Promises and Challenges

El-Kamel¹,

Ashour²

2023

Advanced Drug Delivery Systems

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Ocular diseases include various anterior and posterior segment diseases. Due to the unique anatomy and physiology of the eye, efficient ocular drug delivery is a great challenge to researchers. The emerging nanoscience is playing an important role in the development of novel strategies for ocular disease management. Various active molecules have been designed to associate with nanocarriers to overcome ocular barriers and interact with certain ocular tissues. In this chapter, highlights will be made on barrier to intraocular delivery, general pathways for ocular absorption, and factors affecting intraocular bioavailability. The recent attempts of nanotechnology for treating anterior and posterior ocular diseases will be explored. This will include nanomicelles, nanoparticles, nanosuspensions, vesicular systems, in situ gel, dendrimers, contact lenses, implants, microneedles, and cell-based delivery systems. In addition, gene-based ocular delivery systems will be discussed. In this chapter, we will also provide a comprehensive overview of drug-device combinations used for ocular diseases such as glaucoma, dry eye disease, infections, and inflammations. Furthermore, drug delivery devices for ocular surgeries are discussed. Finally, challenges and future prospective of ocular delivery systems will be explored.

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Effects of Antifungal Soaked Silicone Hydrogel Contact Lenses on Candida albicans in an Agar Eye Model

Abstract: For NA-SA and NA-LB, there was limited growth of C. albicans observed on the eye models even after 48 hr. Under the microscope, the cell morphology differ noticeably between each testing condition, and is dependent on drug-lens combinations.

Cited by 11 publications

References 33 publications

Release of Moxifloxacin from Contact Lenses Using an In Vitro Eye Model: Impact of Artificial Tear Fluid Composition and Mechanical Rubbing

Release of Moxifloxacin from Contact Lenses Using an In Vitro Eye Model: Impact of Artificial Tear Fluid Composition and Mechanical Rubbing

Development of an Eye Model With a Physiological Blink Mechanism

Recent Strategies for Ocular Drug Delivery: Promises and Challenges

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