Effects of antiestrogens on the estrogen-regulated pS2 RNA and the 52- and 160-kilodalton proteins in MCF7 cells and two tamoxifen-resistant sublines.

Westley, Bruce R.; May, Feb; Brown, Anthony M.; Krust, Andrée; Chambon, Pierre; Lippman, Marc E.; Rochefort, Henri

doi:10.1016/s0021-9258(18)90922-1

Cited by 106 publications

(2 citation statements)

References 20 publications

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“…A ds-cDNA clone corresponding to an estrogen-stimulated MCF7 RNA has recently been isolated (9). The sequence (10) and the distribution and hormonal regulation of the mRNA have been studied (11,12,13).…”

Section: Introductionmentioning

confidence: 99%

Cloning of cDNA sequences of a progestin-regulated mRNA from MCF7 human breast cancer cells

Chalbos¹,

Westley²,

May³

et al. 1986

Nucleic Acids Research

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A cDNA clone corresponding to an mRNA regulated by the progestin R5020, has been isolated by differential screening of a cDNA library from the MCF7 breast cancer cell line, which contains estrogen and progesterone receptors. This probe hybridized with a single species of poly A + RNA of 8-kb molecular weight as shown by Northern blot analysis and could also be used to total RNA preparation. This recombinant clone hybridized specifically to an mRNA coding for a 250,000 daltons protein when translated in vitro. This protein was identical to the 250 kDa progestin-regulated protein that we previously described (Biochem. Biophys. Res. Commun. 121, 421-427, 1984) as shown by immunoprecipitation with specific rabbit polyclonal antibodies. Dose-response curve and specificity studies show that the accumulation of the Pg8 mRNA and that of the 250-kDa protein was increased by 5 to 30-fold following progestin treatment and that this effect was mediated by the progesterone receptor. Time course of induction indicated that the accumulation of mRNA was rapid and preceded that of the protein. This is the first report on a cloned cDNA probe of progestin-regulated mRNA in human cell lines.

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Section: Introductionmentioning

confidence: 99%

Cloning of cDNA sequences of a progestin-regulated mRNA from MCF7 human breast cancer cells

Chalbos¹,

Westley²,

May³

et al. 1986

Nucleic Acids Research

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“…Cathepsin D is a lysosomal protease which has been identified in numerous breast cancer cell lines and in mammary tumors (Westley & Rochefort, 1980;Westley et al, 1984Westley et al, , 1989Morisset et al, 1986;Cavailles et al, 1988Cavailles et al, , 1989Cavailles et al, , 1991Cavailles et al, , 1993May et al, 1993;Augereau et al, 1994;Krishnan et al, 1994Krishnan et al, , 1995. Several studies have demonstrated that high levels of cathepsin D in primary mammary tumors are associated with poor prognosis for disease free survival of these patients (Spyratos et al, 1989;Thorpe et al, 1989;Tandon et al, 1990;Kute et al, 1992;Rochefort, 1992;Krishnan et al, 1995).…”

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confidence: 99%

Identification of a Functional Imperfect Estrogen-Responsive Element in the 5‘-Promoter Region of the Human Cathepsin D Gene

et al. 1997

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17beta-Estradiol (E2) induces cathepsin D gene expression in MCF-7 human breast cancer cells. Previous studies have identified an Sp1-imperfect estrogen-responsive element (ERE) half-site [GGGCGG(N)23ACGGG] (-199 to -165) in the promoter region which forms an Sp1-estrogen receptor (ER) complex and confers E2 responsiveness on the corresponding Sp1-ERE-chloramphenicol acetyl transferase (CAT) construct. Further analysis of downstream regions of the promoter identified a CGCCC(N)3TGACC sequence (-119 to -107) which is homologous to the adenovirus major late promoter element (MLPE) and binds the ER to form a retarded band in a gel electrophoretic mobility shift assay. The corresponding promoter-CAT construct is also E2-inducible. The MLPE resembles an imperfect palindromic ERE containing imperfect (5') and perfect (3') ERE half-sites; analysis of oligonucleotides with mutations in these half-sites shows that only the perfect ERE half-site is required for binding the ER, whereas both sites are required for transactivation. In vivo exonuclease III footprinting showed that treatment with E2 also enhanced binding at the MLPE site. Identification of this second functional enhancer sequence in the 5'-promoter region of cathepsin D is consistent with the increasingly complex cell-specific regulation of hormone-responsive genes.

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Evaluation of cathepsin D immunostaining in colorectal adenocarcinoma

et al. 1997

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Background and Objectives Cathepsin D (CD), an estrogen‐regulated lysosomal protease, has been detected in a variety of tissues. CD expression has been correlated with the invasive potential of breast cancer, acting as an autocrine mitogen or as a protease that degrades the extracellular matrix. The role of CD expression in predicting prognosis or invasive potential in colorectal carcinomas is mostly unknown. Methods CD immunohistochemical expression was studied in 60 surgical specimens of colon adenocarcinomas. A three‐step avidin biotinylated, horseradish immuno‐peroxidase (ABC‐HRP) staining technique was performed on 4 μm paraffin‐embedded tissue sections with a polyclonal antibody to CD. Results Carcinoma cells showed positive CD immunostaining in 41.6% of adenocarcinomas (50%, 43.7%, 37.5%, and 25% of Dukes' Stage A, B, C, and D, respectively). Nonneoplastic stromal cells demonstrated positive staining in 68.3% of the adenocarcinoma specimens (37.5%, 62.5%, 91.6%, and 75% of Stage A, B, C, and D, respectively). Patients with colorectal carcinomas exhibiting simultaneously negative and positive CD expression in malignant and stromal cells, respectively, had a worse 5‐year overall survival (P < 0.05). The mean 5‐year survival of the 16 patients overexpressing CD in nonneoplastic stromal cells (>15% of stromal cells positive for CD) was significantly worse in comparison with the rest of the adenocarcinomas (n = 44) (27.6 ± 4.6 vs. 46 ± 2.7 months, respectively, P < 0.01). Conclusions Expression of CD immunoreactivity by the stromal cells may be associated with a more invasive phenotype. Therefore, CD expression in tumor and stromal cells may serve as an important indicator of progression and guide postoperative treatment. J. Surg. Oncol. 1997;65:242–248. © 1997 Wiley‐Liss, Inc.

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Effects of antiestrogens on the estrogen-regulated pS2 RNA and the 52- and 160-kilodalton proteins in MCF7 cells and two tamoxifen-resistant sublines.

Cited by 106 publications

References 20 publications

Cloning of cDNA sequences of a progestin-regulated mRNA from MCF7 human breast cancer cells

Cloning of cDNA sequences of a progestin-regulated mRNA from MCF7 human breast cancer cells

Identification of a Functional Imperfect Estrogen-Responsive Element in the 5‘-Promoter Region of the Human Cathepsin D Gene

Evaluation of cathepsin D immunostaining in colorectal adenocarcinoma

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