Effects of Anticoagulants and Contemporary Blood Collection Containers on Aluminum, Copper, and Zinc Results

Frank, Elizabeth L.; Hughes, Martin Patrick; Bankson, Daniel D.; Roberts, William L.

doi:10.1093/clinchem/47.6.1109

Cited by 25 publications

(18 citation statements)

References 3 publications

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“…This could be in part due to the different collection methodologies of the cohorts, with the SCOPE cohort using heparinised tubes for plasma collection, whilst the Lyell McEwin cohort used EDTA collection vessels. Previous studies have highlighted that different anticoagulants can significantly impact the concentrations of elements available in plasma; haemolysis, particularly with Fe, of these samples has also shown to affect the concentrations [28,29]. As stated previously, Se concentrations collected in the Lyell McEwin cohort were markedly decreased when compared with those in established reference ranges; however, the concentrations published in the SCOPE study fall within these ranges albeit on the lower end (SCOPE: 71.94 ± 11.06 μg/L (minimum = 42.96 μg/L, maximum = 108.98 μg/L), reference range: 70-150 μg/L) [9,21].…”

Section: Discussionmentioning

confidence: 99%

Elemental Metabolomics for Prediction of Term Gestational Outcomes Utilising 18-Week Maternal Plasma and Urine Samples

McKeating

Clifton

Hurst

et al. 2020

Biol Trace Elem Res

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A normal pregnancy is essential to establishing a healthy start to life. Complications during have been associated with adverse perinatal outcomes and lifelong health problems. The ability to identify risk factors associated with pregnancy complications early in gestation is vitally important for preventing negative foetal outcomes. Maternal nutrition has been long considered vital to a healthy pregnancy, with micronutrients and trace elements heavily implicated in maternofoetal metabolism. This study proposed the use of elemental metabolomics to study multiple elements at 18 weeks gestation from blood plasma and urine to construct models that could predict outcomes such as small for gestational age (SGA) (n = 10), low placental weight (n = 18), and preterm birth (n = 13) from control samples (n = 87). Samples collected from the Lyell McEwin Hospital in Adelaide, South Australia, were measured for 27 plasma elements and 37 urine elements by inductively coupled plasma mass spectrometry. Exploratory analysis indicated an average selenium concentration 20 μg/L lower than established reference ranges across all groups, low zinc in preterm (0.64 μg/L, reference range 0.66-1.10 μg/L), and higher iodine in preterm and SGA gestations (preterm 102 μg/L, SGA 111 μg/L, reference range 40-92 μg/L). Using random forest algorithms with receiver operating characteristic curves, low placental weight was predicted with 86.7% accuracy using plasma, 78.6% prediction for SGA with urine, and 73.5% determination of preterm pregnancies. This study indicates that elemental metabolomic modelling could provide a means of early detection of at-risk pregnancies allowing for more targeted monitoring of mothers, with potential for early intervention strategies to be developed.

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Section: Discussionmentioning

confidence: 99%

Elemental Metabolomics for Prediction of Term Gestational Outcomes Utilising 18-Week Maternal Plasma and Urine Samples

McKeating

Clifton

Hurst

et al. 2020

Biol Trace Elem Res

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“…On the other hand, in the study of Frank et. al., [12] in which the Zn level in serum and several types of plasma samples were compared, significantly higher Zn level was detected in EDTA plasma. C Zn / mg mL -1 The higher level of Zn in EDTA plasma was related to the contamination of collection container / anticoagulant with Zn.…”

Section: Influence Of Anticoagulants On Zn Level Detected In Plasmamentioning

confidence: 99%

“…al. [12] however, citrate plasma was not included in that study. Therefore, in this study, the Zn level detected in EDTA, heparin and citrate plasma samples, was compared to the Zn level detected in serum samples.…”

Section: Influence Of Anticoagulants On Zn Level Detected In Plasmamentioning

confidence: 99%

“…al. [12] glass collection tubes (producer was not specified) were used, whereas in this study collection tubes were made of PET plastic (as specified by the producer). It can thus be concluded that the blood collection tube production technique improved over time (2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012)(2013)(2014)(2015)(2016)(2017)(2018), and that no contamination occurs as a consequence.…”

Section: Influence Of Anticoagulants On Zn Level Detected In Plasmamentioning

confidence: 99%

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Impact of Anticoagulants on Assessment of Zinc in Plasma

Jablan¹,

Rajković²,

Inić³

et al. 2018

Croat. Chem. Acta

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The aim of this study was to compare zinc (Zn) level in EDTA, heparin, citrate plasma and serum. The blood samples from the same healthy volunteers (n = 10) were sampled by use of different anticoagulants (EDTA, heparin and citrate) or with no anticoagulant. Zn level was determined by flame atomic-absorption spectrometer (AAnalyst 800, PerkinElmer, USA). Zn level in EDTA, heparin, citrate plasma and serum was in reference range (0.7 to 1.5 μg mL-1), and the difference between sample groups was not observed (P = 0.079). However, correlation was observed only between Zn level in heparin plasma and citrate plasma (rs = 0.72; P = 0.016) and citrate plasma and serum (rs = 0.76; P = 0.012). In conclusion, in Zn blood analysis, sample collection step is important and should be observed when planning study and when comparing the results of Zn level from various studies.

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“…Sedikit kekurangan dalam penanganan sampel sejak pengambilan sampai analisis dapat turut serta memengaruhi hasil. 18,19 Sarana pemeriksaan kadar seng plasma yang tersedia di laboratorium rumah sakit tempat penelitian berlangsung dapat meminimalkan perubahan kadar seng plasma yang terjadi, karena dapat segera dilakukan pemeriksaan. Persediaan seng tubuh sensitif terhadap jumlah seng yang diabsorpsi dari makanan, sehingga makanan yang dimakan subjek sangat memengaruhi kadar seng plasmanya.…”

Section: Pembahasanunclassified

Hubungan Kadar Seng Plasma dengan Derajat Penyakit Pneumonia

Winarni¹,

Rachmadi²,

Sekarwana³

2012

mkb

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Abstrak Pneumonia merupakan masalah kesehatan utama anak di dunia dan sebagai penyebab terbanyak morbiditas dan mortalitas anak di negara berkembang. Anak dengan defisiensi mikronutrien termasuk seng berisiko tinggi terjadi pneumonia, karena gangguan sistem imun. Penelitian ini bertujuan mengetahui hubungan kadar seng plasma dengan pneumonia, pneumonia berat, dan sangat berat pada anak usia 2-59 bulan. Penelitian observasi analitik dengan rancangan potong lintang dilakukan bulan Agustus sampai November 2009 di Departemen Ilmu Kesehatan Anak RS Dr. Hasan Sadikin Bandung, RS Ujung Berung, dan RS Cibabat. Subjek harus memenuhi kriteria diagnosis klinis pneumonia menurut World Health Organization (WHO) Indonesia dan berusia 2-59 bulan. Pengambilan sampel darah untuk pemeriksaan kadar seng plasma dilakukan saat penderita datang. Analisis data menggunakan uji eksak Fisher dan untuk melihat hubungan kadar seng plasma dengan derajat pneumonia menggunakan uji Mann-Whitney. Dari total 42 subjek yang memenuhi kriteria inklusi, didapatkan 1 (2%) anak pneumonia, 32 (76%) pneumonia berat, dan 9 (22%) pneumonia sangat berat. Terdapat perbedaan bermakna (p=0,032) kadar seng plasma antara kelompok pneumonia berat dan sangat berat dengan median 96,685 μg/dL (57,32-195,66 μg/ dL) untuk penumoia berat dan 80,240 μg/dL (63,01-111,84 μg/dL) untuk pneumonia sangat berat. Penelitian ini menunjukkan bahwa bahwa kadar seng plasma memiliki hubungan dengan pneumonia berat dan sangat berat pada anak usia 2-59 bulan. [MKB. 2012;44(4):213-17]. Abstract Pneumonia is a major health problem affecting children all over the world and remains a major cause of childhood morbidity and mortality in developing countries. Children with micronutrients deficiency including zinc, which might cause immune system disorder, have higher risk to have pneumonia. The aim of this study was to investigate the association between plasma zinc level and pneumonia, severe, and very severe pneumonia in children aged 2-59 months. This observational analytic with cross-sectional study was performed at the Subjects of this study were 2-59-month-old children who meet the WHO Indonesian classification for pneumonia. Blood samples for plasma zinc examination were collected on admission. Data were analysed using exact Fisher and Mann-Whitney test for the association between plasma zinc level and severity of pneumonia. A total of 42 subjects were enrolled, 1 (2%) child were classified as having pneumonia, 32 (76%) children with severe, and 9 (22%) with very severe pneumonia. There were significant differences (p=0.032) in plasma zinc levels between severe and very severe pneumonia with a median of 96.685 μg/dL (57.32-195.66 μg/dL) for severe pneumonia and 80.240 μg/dL (63.0-111.84 μg/dL) for very severe pneumonia. This study shows an association between plasma zinc levels and severe and very severe pneumonia in children aged 2-59 months. [MKB. 2012;44(4):213-17].

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Effects of Anticoagulants and Contemporary Blood Collection Containers on Aluminum, Copper, and Zinc Results

Cited by 25 publications

References 3 publications

Elemental Metabolomics for Prediction of Term Gestational Outcomes Utilising 18-Week Maternal Plasma and Urine Samples

Elemental Metabolomics for Prediction of Term Gestational Outcomes Utilising 18-Week Maternal Plasma and Urine Samples

Impact of Anticoagulants on Assessment of Zinc in Plasma

Hubungan Kadar Seng Plasma dengan Derajat Penyakit Pneumonia

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