Effects of Ankaferd Hemostat on Helicobacter pylori strains and antibiotic resistance

Abstract

Introduction Ankaferd hemostat (ABS; Ankaferd blood stopper, İstanbul, Turkey) is a folkloric medicinal plant extract. ABS has been conventionally used in Anatolia as a hemostatic agent for centuries (1). ABS contains a standardized combination of the plants Glycyrrhiza glabra, Thymus vulgaris, Alpinia officinarum, Vitis vinifera, and Urtica dioica. All of these plants have effects on the endothelium, the cellular components of blood, the development of new blood vessels, cell proliferation, and cell mediators (2,3). The hemostatic effect of ABS depends upon the quick promotion of a protein network, particularly fibrinogen gamma, in relation to the erythrocyte aggregation (1). In addition to hemostatic functions, antiinflammatory (4), antiinfective (5), antifungal (6), and antioxidative (4) effects have been demonstrated for ABS. Thymus vulgaris has bacteriostatic activity for gram-positive and gram-negative bacteria (7-10). Likewise, Glycyrrhiza glabra, Vitis vinifera, and Alpinia officinarum have been shown to be antibacterial agents (11,12). Urtica dioica also has significant antibacterial activity against Streptococcus pyogenes, Staphylococcus aureus, and Staphylococcus epidermidis (12). ABS is currently licensed for numerous bleeding lesions of GIS pathologies, such as peptic ulcers (13), fundal varices (14), dieulafoy lesions (15,16), radiation colitis (17), rectal ulcers (18) and nonvariceal upper gastrointestinal bleeding (19). Most of the bleeding lesions were controlled with ABS in all patient groups (20-23). Furthermore, ABS is a potent hemostatic drug for controlling malignant gastrointestinal (GI) tumors. It has successful antineoplastic effects on colon cancer (as represented by the in vitro effects on CaCo-2 cells) (24). It significantly decreases tumor microvessel density (25). Almost 70% of gastric ulcer and 85% to 95% of duodenal ulcer patients have coexisting H. pylori infections (26,27). It is well recognized that H. pylori eradication therapy can reduce the repetition of peptic ulcer. Several different studies support the important role played by H. pylori in mucosa-associated lymphoid tissue (MALT) lymphomagenesis. Chronic infection with H. pylori is significantly related with the induction of gastric lymphoid Background/aim: Ankaferd hemostat (ABS; Ankaferd blood stopper, İstanbul, Turkey) is a folkloric medicinal plant extract. The aim of this study was to determine the effect of Ankaferd hemostat (ABS) on the fate of Helicobacter pylori strains. The study also aims to determine alterations in the antimicrobial resistance of three different H. pylori strains in response to ABS exposure. Materials and methods: H. pylori Strain 1 was obtained from the culture collection ATCC 43504 and passaged three times for viability. Strain 2 was isolated from a gastric ulcer patient and Strain 3 was isolated from a gastritis patient. 1% of ABS was added to all of the strains and antimicrobial susceptibility was observed on 30 and 60 min after application. Results: The efficacy of ABS solutions in achievi...

Ankaferd hemostat: from molecules to medicine

“…The efficiency of ABS on H. pylori was also demonstrated. Possible anti-H. pylori effect of ABS will broaden the therapeutic spectrum of the drug in GI lesions as peptic ulcer disease (PUD) and lymphoid tissue (MALT) lymphomagenesis [73]. In vitro activity of ABS against M. Tuberculosis isolates was evaluated.…”

Ankaferd hemostat (ABS) as a potential mucosal topical agent for the management of COVID-19 syndrome based on its PAR-1 inhibitory effect and oestrogen content

“…ABS is approved as a topically applied hemostatic agent for the management of post-surgical/dental bleedings and healing of the infected inflammatory mucosal wounds [7] , [8] . Although initally presented as a hemostatic agent, compelling body of evidence suggest that ABS has anti-inflammatory, anti-infective and anti-oxidative properties in distinct disease states [9] , [10] , [11] , [12] , [13] , [14] . Moreover, Urtica dioica which is the major component of ABS possesses broad-spectrum antiviral properties including SARS-CoV strains [15] .…”

“…Moreover, Urtica dioica which is the major component of ABS possesses broad-spectrum antiviral properties including SARS-CoV strains [15] . There are preliminary evidence that ABS has in vitro anti-viral actions on the Bovine Herpes virus type 1 (BHV-1) [13] as well as other established anti-infective properties in a wide variety of pathogens [11] , [12] , [13] , [16] , [17] . Most importantly, topical ABS applications for severe oral mucositis in patients with pediatric and adult cancer receiving chemotherapy/ radiotherapy [18] , [19] and mucosal healing of the pulmonary tissue in lung-bleeding patients [14] demonstrated the efficacy and safety of the drug for mucosal disease control in clinical backgrounds.…”

Histopathological and mechanical effects of Ankaferd Blood Stopper® on wound healing in rats: an experimental model

“…ABS has been approved for the management of external hemorrhage and dental surgery by the Ministry of Health in Turkey [9]. ABS is a promising hemostatic and possesses regenerative, antineoplastic, and antimicrobial properties against multidrug resistant bacteria [10][11][12]. ABS is a hemostatic agent used to control postoperative bleeding in patients with various bleeding disorders [13,14].…”