Effects of angiotensin II on fluid transport, transmural potential difference and blood flow by rat jejunum in vivo.

Bolton, John R.; Munday, K. A.; Parsons, B. J.; York, Barbara

doi:10.1113/jphysiol.1975.sp011197

Cited by 51 publications

(44 citation statements)

References 23 publications

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“…Angiotensin II (ANG II) regulates sodium and water transport across epithelial cells in the kidney and gastrointestinal tract (1)(2)(3)(4)(5). In the jejunum, low concentrations of ANG II stimulate sodium and water absorption while high concentrations inhibit absorption and/or stimulate secretion (3).…”

Section: Introductionmentioning

confidence: 99%

Compartmentalization of extracellular cGMP determines absorptive or secretory responses in the rat jejunum

Jin

Siragy²,

Guerrant³

et al. 1999

J. Clin. Invest.

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Angiotensin II (ANG II) regulates sodium and water transport across epithelial cells in the kidney and gastrointestinal tract (1-5). In the jejunum, low concentrations of ANG II stimulate sodium and water absorption while high concentrations inhibit absorption and/or stimulate secretion (3). We have recently demonstrated that ANG II stimulates absorption in the jejunum by an action at the AT 2 receptor involving the generation of guanosine cyclic 3′,5′-monophosphate (cGMP) (3,6,7).In this study, we hypothesized that ANG II increases jejunal sodium and water absorption by increasing the synthesis of nitric oxide (NO) and stimulating soluble guanylyl cyclase. However, since cGMP is thought to mediate secretory, not absorptive, responses in the gastrointestinal tract when particulate guanylyl cyclase (GC-C) is stimulated by the heat-stable enterotoxin of Escherichia coli (STa) or guanylin or uroguanylin (8, 9), we hypothesized that the sodium and water transport response, absorptive or secretory, is associated with a compartmentalized cGMP response via either soluble or particulate guanylyl cyclase. If so, STa would stimulate secretion by stimulating particulate guanylyl cyclase on the apical cell membrane of transporting epithelial cells. In contradistinction, nitric oxide would stimulate absorption via soluble guanylyl cyclase from the serosal side, and cGMP would be released predominantly across the basolateral membrane of epithelial cells into the interstitial space. We introduced both in situ loop and microdialysis techniques to test these hypotheses. MethodsAnimal preparation. Male Wistar rats weighing 200 ± 15 g were obtained from Harlan Bioproducts Inc. (Indianapolis, Indiana, USA). Rats were housed in a room with 12-h light-dark cycles and maintained on standard rat chow containing 0.28% NaCl or low-sodium chow containing 0.08% NaCl (BioServe Biotechnologies, Frenchtown, New Jersey, USA). Rats were fasted overnight before the study.Operative procedure. Rats (n = 8 in each group) were anesthetized with pentobarbital sodium 70 mg/kg intraperitoneally (Veterinary Laboratories, Inc., Lenexa, Kansas). The trachea and left jugular vein were cannulated. A venous cannula (PE 50) was connected to a Harvard 975 infusion pump (Harvard Apparatus Co., South Natick, Massachusetts, USA) through which lactated Ringer's solution (vehicle) and drugs were infused. A ventral midline celiotomy also was performed and the jejunum was prepared in two different ways as described below.Single-pass perfusion. These studies were performed according to previous publications (10, 11). An inlet cannula was placed just below the duodenal-jejunal flexure and the outlet cannula was 15 cm distal to the inlet cannula. The lumen just proximal and distal to the cannulated segment was tied off. To measure water transport, lactated Ringer's solution containing [ 14 C]inulin (15,000 dpm/ml; 2.2 µCi/mg specific activity; New England Nuclear, Boston, Massachusetts, USA) and phenol red (50 mg/liter, Sigma Chemical Company; St. Louis, Missouri, We ex...

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Section: Introductionmentioning

confidence: 99%

Compartmentalization of extracellular cGMP determines absorptive or secretory responses in the rat jejunum

Jin

Siragy²,

Guerrant³

et al. 1999

J. Clin. Invest.

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“…Intestinal fluid transport was measured in vivo by the method of Bolton, Munday, Parsons & York (1975). Fluid absorption in anaesthetized animals (given as ml.…”

Section: P Proceedings Of Thementioning

confidence: 99%

Proceedings of the Physiological Society, 16‐17 July 1982, Cambridge Meeting: Communications

1982

The Journal of Physiology

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In three experiments 2 #g ACTH potentiated the inhibitory effects of morphine.This opiate-like action of ACTH was even more apparent at higher doses. The pressure response to hypertonic sodium chloride was substantially reduced and in one case completely abolished by 10 jug ACTH (n = 3) in the absence of morphine, an effect that was antagonized by naloxone.These experiments show that opiate inhibition of the release of neurohypophysial hormones may be attenuated by low doses of ACTH. At higher doses ACTH appears to have an opiate-like action. This suggests that the effects ofACTH and endorphin-like peptides on the release of neurohypophysial hormones may depend upon their relative concentrations -perhaps as a consequence of the differential processing of their common precursor.

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“…Department of Physiology and Biochemistry, University of Southampton Recent evidence has shown that noradrenaline stimulates electroneutral intestinal absorption in vitro (Brasitus, Field & Kimberg, 1977). Furthermore the hormone angiotensin stimulates electroneutral fluid absorption in vivo in the absence of changes in blood flow (Bolton, Munday, Parsons & York, 1975), and also releases noradrenaline both from sympathetic nerve endings and from the adrenal medulla (Peach, 1974). The following experiments were carried out to compare the effects of angiotensin and noradrenaline on intestinal fluid transport in vivo.…”

Section: Pmentioning

confidence: 99%

“…Closed sacs of rat jejunum filled with Krebs' bicarbonate saline containing either [3H]inulin or [14C]polyethylene glycol, to act as a nonabsorbable marker, were prepared as described by Bolton et al (1975). A control period of isotonic saline infusion at 1 ml.…”

Section: Pmentioning

confidence: 99%

Communications

1977

The Journal of Physiology

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Effects of angiotensin II on fluid transport, transmural potential difference and blood flow by rat jejunum in vivo.

Cited by 51 publications

References 23 publications

Compartmentalization of extracellular cGMP determines absorptive or secretory responses in the rat jejunum

Compartmentalization of extracellular cGMP determines absorptive or secretory responses in the rat jejunum

Proceedings of the Physiological Society, 16‐17 July 1982, Cambridge Meeting: Communications

Communications

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