1985
DOI: 10.1111/j.1600-0773.1985.tb00004.x
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Effects of Anaesthetics on Protein Synthesis in Isolated Rat Hepatocytes: Inhibition by Diethyl Ether in Contrast to No Influence by Pentobarbital and Fentanyl

Abstract: Hepatocytes were isolated from livers of fasted rats by a two-step Ca+ +-free/collagenase perfusion method. Suspensions of parenchymal liver cells were incubated in the absence and presence of three different anaesthetics, diethyl ether, pentobarbital and fentapyl at various concentrations. Their influence on the hepatocytes was monitored by measuring protein synthesis as the incorporation of L-(UJ4C) valine (50 mCi/ mol, 4.2 mM) into liver proteins. Diethyl ether representing anaesthetics mainly affecting cel… Show more

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Cited by 9 publications
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