Effects of anaesthesia on proliferation, invasion and apoptosis of LoVo colon cancer cells in vitro

Abstract

Tumour cell proliferation, invasion and apoptosis are crucial steps in tumour metastasis. We evaluated the effect of serum from patients undergoing colon cancer surgery receiving thoracic epidural and propofol anaesthesia on colon cancer cell biology. Patients were randomly assigned to receive propofol anaesthesia with a concomitant thoracic epidural (PEA, n = 20) or sevoflurane anaesthesia with opioid analgesia (SGA, n = 20). Venous blood was obtained before induction of anaesthesia and 24 hours postoperatively. The LoVo colon cancer cells were cultured with patient serum from both groups and the effects on proliferation, invasion and apoptosis were measured. Twenty-four hours after surgery, the absorbance value of LoVo cells at 10% serum concentration from PEA was decreased when compared with SGA (0.302 (0.026) vs 0.391 (0.066), p = 0.005). The inhibitory rate of LoVo cells at 10% serum concentration from PEA was higher than that from SGA (p = 0.004) 24 h after surgery. The number of invasive LoVo cells at 10% serum concentration from PEA was reduced when compared with SGA (44 (4) vs 62 (4), p < 0.001). Exposure of LoVo cells to postoperative serum from patients receiving PEA led to a higher luminescence ratio (apoptosis) than those receiving SGA (0.36 (0.04) vs 0.27 (0.05), p < 0.001). Serum from patients receiving PEA for colon cancer surgery inhibited proliferation and invasion of LoVo cells and induced apoptosis in vitro more than that from patients receiving SGA. Anaesthetic technique might influence the serum milieu in a way that affects cancer cell biology and, thereby, tumour metastastasis.

The erector spinae plane block: plane and simple

“…Multimodal analgesia is critical both to ERAS and to achieve the target of DREAMing (DRinking, EAting and Mobilising) . Regional anaesthesia complements and enhances multimodal analgesia for abdominal surgery, with its role increasingly recognised , and may indeed alter outcomes . However, which regional anaesthetic technique is most appropriate, efficacious, safe, timely and consistent in the hands of most practitioners remains highly debated.…”

Propofol Disrupts Aerobic Glycolysis in Colorectal Cancer Cells via Inactivation of the NMDAR-CAMKII-ERK Pathway

“…We found that propofol had little inhibitory effect on the glucose metabolism of normal cells. Previous studies showed that propofol deceases the metastasis and progression of many cancers, including pancreatic cancer [26], breast cancer [27], colon cancer [28], gastric cancer [22], and cervical cancer [29]. However, the specific role of propofol in glucose metabolism in cancer cells remains poorly understood.…”

Safe performance of peripheral regional anaesthesia: the significance of ultrasound guidance

“…Multimodal analgesia is critical both to ERAS [2] and to achieve the target of DREAMing (DRinking, EAting and Mobilising) [7]. Regional anaesthesia complements and enhances multimodal analgesia for abdominal surgery, with its role increasingly recognised [8], and may indeed alter outcomes [9][10][11]. However, which regional anaesthetic technique is most appropriate, efficacious, safe, timely and consistent in the hands of most practitioners remains highly debated.…”