Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts

Akcha, Farida; Barranger, Audrey; Bachère, Evelyne; Berthelin, Clothilde Heude; Piquemal, David; Alonso, Pascal; Rondon, Rodolfo; Dimastrogiovanni, Giorgio; Porte, Cinta; Ménard, D.; Szczybelski, Ariadna S.; Benabdelmouna, Abdellah; Auffret, Michel; Rouxel, Julien; Burgeot, Thierry

doi:10.1007/s11356-015-5969-2

Cited by 28 publications

(18 citation statements)

References 54 publications

(65 reference statements)

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“…Our study tends to reinforce the converging evidences supporting the fact that Diuron may be a potential tumorigenic substance. This effect was direct such as already reported to the bladder [14,15], urothelial [16], skin [17,18] and mammary [15][16][17][18][19] carcinogenesis or indirect via the decrease of immunosurveillance phenomenon. However, in the absence of study in human, our study should not be considered as an absolute proof of the guilt of Diuron in the occurrence of cancer but as a scientific rationale incriminating a potential cellular mechanism that may be at the origin of the initiation of cancer.…”

Section: Resultssupporting

confidence: 51%

“…Literature reports that Diuron affects the global DNA methylation level [15][16][17]; we first investigated whether Diuron exposure could affect the global DNA methylation level of the pDC-like cell line CAL-1. For this purpose, CAL-1 cells were expose to 25 mg/L of Diuron for 72 h since this herbicide and four of its metabolites had a total concentration as high as 100 mg/L in plasma and urine [25] (Fig.…”

Section: Diuron Exposure Promotes the Tet2-mediated Dna Hypomethylatimentioning

confidence: 99%

“…but also human health. Thus, literature reports that Diuron modulates the methylome of oysters [15][16][17], and affects the multixenobiotic resistance activity in Zebrafish [18]. Reports also mention a health risk for population exposed to Diuron [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

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Diuron modulates the DNA methylation status of the ILT7 and TRAIL/TNFSF10 genes and decreases the killing activity of plasmacytoid dendritic cells

et al. 2019

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Background: Plasmacytoid dendritic cell (pDC) is described as the Swiss knife of immune system. Thus, the understanding of aberrant epigenetic reprogramming of genes governing the pDC functionality by pollutants appears such as an attractive research point. Results: Our study has investigated the effect of Diuron (an herbicide) on the pDC-killing activity towards cancer cells. Thus, we observed that the Diuron exposure of pDC promotes a context of global DNA hypomethylation, which is associated with a phenotype of decrease of the killing activity of pDC towards cancer cells. At molecular level, our data associated the Diuron-induced global DNA hypomethylation with the elevated expression of TET2, an epigenetic player involved in DNA demethylation processes, and the decrease of the pDC-killing activity with the decrease of TRAIL expression and the increase of ILT7 expression. Conclusions: Thus, our article reports that a pollutant (Diuron) induces an epigenetic reprogramming of a subtype of immune cell (pDC), which decreases its killing activity towards tumors cells. In some context, this mechanism might be conducive to the initiation of pathologies.

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Section: Resultssupporting

confidence: 51%

Section: Diuron Exposure Promotes the Tet2-mediated Dna Hypomethylatimentioning

confidence: 99%

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Diuron modulates the DNA methylation status of the ILT7 and TRAIL/TNFSF10 genes and decreases the killing activity of plasmacytoid dendritic cells

et al. 2019

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“…Cellular or molecular analysis, such as metabolomics, offers insights regarding broad molecular changes that could occur in an individual as a result of chemical exposure, and thus provides information that may relate to the mechanistic underpinnings of observed higher level effects (Lin et al 2007; Bouhifd et al 2013). Because pesticides can have physiological (Yasmin and D'Souza 2010), molecular (Van Meter et al 2018), and cellular (Akcha et al 2016) effects on nontarget species, the integration of multiple approaches to ecotoxicology studies will provide a better understanding of the overall pesticide toxicity.…”

Section: Introductionmentioning

confidence: 99%

Polyps of the Jellyfish Aurelia aurita Are Unaffected by Chronic Exposure to a Combination of Pesticides

Olguín-Jacobson

Pitt

Carroll

et al. 2020

Enviro Toxic and Chemistry

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Pesticides are a major contaminant in coastal waters and can cause adverse effects in marine invertebrates such as jellyfish. Most studies have investigated short‐term responses of organisms to unrealistically high concentrations of pesticides; however, chronic exposure to persistent low concentrations, which are more likely to occur in the environment, are rarely analyzed. We tested the response of polyps of the moon jellyfish Aurelia aurita to environmental concentrations of the herbicide atrazine and the insecticide chlorpyrifos, individually and in combination, over 9 wk. We hypothesized that exposure to individual pesticides would reduce rates of asexual reproduction and alter polyps' metabolite profiles, and that the results would be more severe when polyps were exposed to the combined pesticides. Polyps survived and reproduced (through budding) in all treatments, and no differences among treatments were observed. Proton nuclear magnetic resonance spectroscopy revealed no difference in profiles of polar metabolites of polyps exposed to the individual or combined pesticides. Our results suggest that A. aurita polyps are unaffected by chronic exposure to atrazine and chlorpyrifos at concentrations recommended as being protective by current Australian water quality guidelines. Environ Toxicol Chem 2020;39:1685–1692. © 2020 SETAC

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“…In addition, diuron is suspected to be a carcinogenic and genotoxic compound for both humans and aquatic organisms such as fish (Huovinen et al, 2015, Canna-Michaelidou andNicolaou, 1996). In C. gigas, direct exposure of adult oysters to an environmentally realistic concentration of diuron (from 0.05 µg/L upwards) has a genotoxic effect in somatic, germ and gamete cells (Akcha et al, 2016). Moreover, an aneugenic effect and a negative impact on development have been observed in the next spat generation (Barranger et al, 2014, Bouilly et al, 2007, Barranger et al, 2015.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron

et al. 2016

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The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlining the importance of environment quality. The impact of water pollution has been pointed out and one of the pollutants, the genotoxic herbicide diuron, occurs at high concentrations all along the French coasts. Previous work has revealed that a parental exposure to diuron had a strong impact on hatching rates and offspring development even if spats were not exposed to diuron themselves. In this study, we explored for the first time the transcriptional changes occurring in oyster spats (non exposed) originating from genitors exposed to an environmentally relevant concentration of diuron during gametogenesis using the RNAseq methodology. We identified a transcriptomic remodeling revealing an effect of the herbicide. Different molecular pathways involved in energy production, translation and cell proliferation are particularly disturbed. This analysis revealed modulated candidate genes putatively involved in response to oxidative stress and mitochondrial damage in offspring of genitors exposed to diuron. Complementary measures of the activity of enzymes involved in these latter processes corroborate the results obtained at the transcriptomic level. In addition, our results suggested an increase in energy production and mitotic activity in 5-month-spats from diuron-exposed genitors. These results could correspond to a "catch-up growth" phenomenon allowing the spats from diuron-exposed genitors, which displayed a growth delay at 3 months, to gain a normal size when they reach the age of 6 months. These results indicate that exposure to a concentration of diuron that is frequently encountered in the field during the oyster's gametogenesis stage can impact the next generation and may result in fitness disturbance.

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Effects of an environmentally relevant concentration of diuron on oyster genitors during gametogenesis: responses of early molecular and cellular markers and physiological impacts

Cited by 28 publications

References 54 publications

Diuron modulates the DNA methylation status of the ILT7 and TRAIL/TNFSF10 genes and decreases the killing activity of plasmacytoid dendritic cells

Diuron modulates the DNA methylation status of the ILT7 and TRAIL/TNFSF10 genes and decreases the killing activity of plasmacytoid dendritic cells

Polyps of the Jellyfish Aurelia aurita Are Unaffected by Chronic Exposure to a Combination of Pesticides

Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron

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