Effects of amniotic membrane on the healing of primary colonic anastomoses in the cecal ligation and puncture model of secondary peritonitis in rats

Uludağ, Mehmet; Çıtgez, Bülent; Özkaya, Ozan; Yetkın, Gürkan; Özcan, Ömer; Polat, Nedim; İşgör, Adnan

doi:10.1007/s00384-009-0645-y

Cited by 23 publications

(43 citation statements)

References 44 publications

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“…When the difference was significant, it was performed tests of multiple comparison (Tukey Test) to identify the differences (Table 2). The peritoneum infection is one of the main factors that interfere in the cicatrization of anastomosis performed in colon 28,29,31,35,36 .…”

Section: Discussionmentioning

confidence: 99%

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Hyperbaric oxygen therapy and mechanical resistence of the colonics anastomosis in rats with peritonitis

et al. 2010

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Purpose:To analyze the effects of hyperbaric oxygen therapy (HBO) on the mechanical resistance of anastomosis performed in rats' distal colon presenting peritonitis induced by ligature and cecal punction using the Total Energy Rupture biomechanical test (ETR). Methods: It was used 45 rats divided into three groups of 15 animals each. In Control Group (CG), it was performed anastomosis in distal colon without peritonitis. In Peritonitis Group (PG), it was performed anastomosis six hours after the induction of peritonitis by ligature and cecal punction. In Hyperbaric Chamber Group (HCG), it was performed six hours after the induction of peritonitis by ligature and cecal punction. The animals on CG and PG were kept at place air while the animals on HCG were placed on an experimental hyperbaric chamber in order to inhale oxygen at 100%, two absolute atmospheres, for 120 minutes, for four consecutive days. Euthanasia took place on the fifth day of the experiment. All the animals underwent to Total Energy Rupture Biomechanical Resistance test (ETR). Total Energy Rupture was defined as the necessary internal energy stored up to promote the colon rupture after an external traction force imposition. Results: The Peritonitis Group presents smaller average ETR than Control Group. There was no statistical difference between Peritonitis Group and Hyberbaric Chamber Group. Conclusion: Hyperbaric oxygen therapy did not alter the mechanical resistance of anastomosis performed in distal colon of rats under the presence of peritonitis induced by ligature and cecal puncture. Key words: Anastomosis, Surgical. Colon. Hyperbaric Oxygenation. Peritonitis. Rats. RESUMO Objetivo:Analisar o efeito da oxigenoterapia hiperbárica (HBO) sobre a resistência mecânica de anastomoses realizadas em cólon distal de ratos na presença de peritonite induzida por ligadura e punção cecal utilizando o teste biomecânico de Energia Total de Ruptura (ETR). Métodos: Foram utilizados 45 ratos distribuídos em três grupos de 15 animais. No Grupo Controle (GC), realizou-se anastomose no cólon distal sem peritonite. No Grupo Peritonite (GP), realizou-se anastomose seis horas após a indução da peritonite por ligadura e punção cecal. No Grupo Câmara Hiperbárica (GCH), realizou-se anastomose seis horas após a indução da peritonite por ligadura e punção cecal. Os animais dos GC e GP foram mantidos em ar ambiente. Os animais do GCH foram colocados em uma câmara hiperbárica experimental para inalarem oxigênio a 100%, a duas atmosferas absolutas, durante 120 minutos, por quatro dias consecutivos. A eutanásia ocorreu no quinto dia do experimento. Todos os animais foram submetidos ao Teste de Resistência Biomecânico Energia Total de Ruptura (ETR). A Energia Total de Ruptura foi definida como a energia interna acumulada necessária para promover o rompimento do cólon após a imposição de uma força externa de tração. Resultados: O Grupo Peritonite apresentou menor média de ETR que o Grupo Controle. Não houve diferença estatística entre o Grupo Peritonite e o Grupo C...

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Section: Discussionmentioning

confidence: 99%

“…Besides the mentioned factors, peritonitis is an important factor that can alter the cicatrization of anastomosis in bowels [19][20][21][22][23][24][25][26][27][28][29] .…”

Section: Introductionmentioning

confidence: 99%

Hyperbaric oxygen therapy and mechanical resistence of the colonics anastomosis in rats with peritonitis

et al. 2010

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“…This result is in concordance with the findings of some other studies on the small size animals. [10171819] In a study on 50 Wistar rats, Schimidt et al . proved that “HAM allows the wound to heal by second-intention, with re-establishment of duodenal wall structure.”[10] HAM was also applied in ileal defects in a study on rabbits by Barlas in Turkey, which resulted in less intestinal obstruction because of better healing resulted from formation of neo-mucosa in site of repair.…”

Section: Discussionmentioning

confidence: 99%

“…In addition tissue growth factors and multi-potent cells of HAM can result in angiogenesis and fibroblastic activity thus, wound strength. [29181922]…”

Section: Discussionmentioning

confidence: 99%

Evaluation of repair in duodenal perforation with human amniotic membrane: An animal model (dog)

et al. 2014

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Background:There is a growing tendency toward application of human amniotic membrane (HAM) as a biologic substitute in various tissue injuries where a significant tissue loss is a matter of concern. In gastrointestinal injuries especially duodenal ones, some potential limitations in current surgical techniques contribute to not fully acceptable healing outcomes. Thus, this study was carried out to assess repair with HAM patch for duodenal defect in comparison with simple duodenoraphy in an animal model (dog).Materials and Methods:A total of 15 male German shepherd dogs weighing 23-27 kg were randomly divided into two groups. Group A with 10 dogs, which were a candidate for duodenal repair by amniotic membrane patch and Group B consisted of 5 dogs perform simple duodenorraphy. A precise control was made to match all conditions except surgical technique. Macroscopic and microscopic features of the healed duodenal lumen in both groups were recorded.Results:Gross evaluation revealed no difference in luminal diameter in both groups. Statistical analysis of duodenal diameter between both groups after operation also showed no significant difference (Pv = 0.789). Histological assessment indicated less inflammation with better wound healing in Group A.Conclusion:It seems that repairing duodenal wall defect with HAM would result in better histological outcomes compared with what is seen in simple duodenoraphy in animal models. However, there is no significant difference regarding surgical findings.

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“…[910] In addition, the hypothesis that some features of HAM show variation in different organs encouraged surgeons to utilize this bio-material in gastrointestinal tract surgeries. [1112]…”

Section: Introductionmentioning

confidence: 99%

A new method to repair recto-vaginal fistula: Use of human amniotic membrane in an animal model

et al. 2014

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Background:Recto-vaginal fistula is primarily one of the co-morbidities of vaginal delivery. These patients suffer from persistent malodor vaginal discharge. Various surgical techniques have been employed by surgeons in the course of time. This is the first trial of applying Human Amniotic Membrane (HAM) as a bio-prosthesis in repairing recto-vaginal fistula.Materials and Methods:In a prospective animal study, 8 mixed-breed female dogs weighing 23-27 kg with the age of 12-18 months were selected. They were randomly divided into two groups for standard recto-vaginal fistula repair and fistula repair with human amniotic membrane. The Kruskal-Wallis and Mann Whitney tests were performed to indicate statistical differences.Results:After 6 weeks, fistulas were evaluated both grossly and microscopically. In gross examination, there were no difference between the two groups and healing of fistula seemed to have been occurred in all dogs expect for one which had a persistent patent fistulous tract. Microscopic healing was scored according to epithelialization, collagenization inflammation, ulcer and necrosis of samples. Healing score was significantly higher in the HAM group than the standard group (P = 0.029).Conclusion:Our findings revealed that using HAM as a bio-prosthesis to repair recto-vaginal fistula would result in better surgical and histological outcomes comparing to simple repair.

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Effects of amniotic membrane on the healing of primary colonic anastomoses in the cecal ligation and puncture model of secondary peritonitis in rats

Cited by 23 publications

References 44 publications

Hyperbaric oxygen therapy and mechanical resistence of the colonics anastomosis in rats with peritonitis

Hyperbaric oxygen therapy and mechanical resistence of the colonics anastomosis in rats with peritonitis

Evaluation of repair in duodenal perforation with human amniotic membrane: An animal model (dog)

A new method to repair recto-vaginal fistula: Use of human amniotic membrane in an animal model

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