Effects of ammonium chloride and chloroquine on endocytic uptake of liposomes by Kupffer cells in vitro

Dijkstra, J.W.; Galen, Mieke van; Scherphof, Gerrit L.

doi:10.1016/0167-4889(84)90099-5

Cited by 48 publications

(16 citation statements)

References 29 publications

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“…Lysosomotropic reagents are often utilized to increase transfection efficiency for gene delivery methods that are limited by the ability to induce endosomal escape prior to enzymatic degradation in the lysosome [ 62 – 64 ]. For example, chloroquine, a lysosomotropic agent, can prevent fusion of endosomes and lysosomes, and the resultant DNA degradation in the acidic environment in lysosomes through inhibition of acidification in late endosomes and lysosomes [ 11 , 15 , 62 , 63 , 65 ].…”

Section: Discussionmentioning

confidence: 99%

Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection

et al. 2017

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A recent theory suggests that endocytosis is involved in uptake and intracellular transport of electrotransfected plasmid DNA (pDNA). The goal of the current study was to understand if approaches used previously to improve endocytosis of gene delivery vectors could be applied to enhancing electrotransfection efficiency (eTE). Results from the study showed that photochemically induced endosomal escape, which could increase poly-L-lysine (PLL)-mediated gene delivery, decreased eTE. The decrease could not be blocked by treatment of cells with endonuclease inhibitors (aurintricarboxylic acid and zinc ion) or antioxidants (L-glutamine and ascorbic acid). Chemical treatment of cells with an endosomal trafficking inhibitor that blocks endosome progression, bafilomycin A1, resulted in a significant decrease in eTE. However, treatment of cells with lysosomotropic agents (chloroquine and ammonium chloride) had little effects on eTE. These data suggested that endosomes played important roles in protecting and intracellular trafficking of electrotransfected pDNA.

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Section: Discussionmentioning

confidence: 99%

Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection

et al. 2017

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“…Along this route, liposomes encounter compartments of progressively more acidic pH and are degraded together with their contents once they reach the lysosomes (Dijkstra et al 1984, Yoshimura et al 1995, Scherphof and Kamps 1998. Endocytosis is also the main mechanism by which DNA, as well as oligonucleotide-cationic liposome complexes, are taken up into the cell (Zhou and Huang 1994, Friend et al 1996, Zelphati and Szoka 1996a.…”

Section: Endocytosismentioning

confidence: 99%

Lipid-based systems for the intracellular delivery of genetic drugs

Maurer

Mori²,

Palmer³

et al. 1999

Molecular Membrane Biology

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Currently available delivery systems for genetic drugs have limited utility for systemic applications. Cationic liposome/plasmid DNA or oligonucleotide complexes are rapidly cleared from circulation, and the highest levels of activity are observed in 'first pass' organs, such as the lungs, spleen and liver. Engineered viruses can generate an immune response, which compromises transfection resulting from subsequent injections and lack target specificity. A carrier, which can accumulate at sites of diseases such as infections, inflammations and tumours, has to be a small, neutral and highly serum-stable particle, which is not readily recognized by the fixed and free macrophages of the reticuloendothelial system (RES). This review summarizes lipid-based technologies for the delivery of nucleic acid-based drugs and introduces a new class of carrier systems, which solve, at least in part, the conflicting demands of circulation longevity and intracellular delivery. Plasmid DNA and oligonucleotides are entrapped into lipid particles that contain small amounts of a positively charged lipid and are stabilized by the presence of a polythylene glycol (PEG) coating. These carriers protect nucleic acid-based drugs from degradation by nucleases, are on average 70 nm in diameter, achieve long circulation lifetimes and are capable of transfecting cells.

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“…4 However, the development of liposomes for large-scale clinical applications has been met with two major challenges: rapid elimination by the reticuloendothelial system (RES) and inefficient drug release. [5][6][7][8] Various strategies have been developed. The circulation time of liposomes in the bloodstream can be prolonged with the insertion of sterically stabilizing amphiphiles such as polyethylene glycol (PEG)-modified phospholipids.…”

Section: Introductionmentioning

confidence: 99%

Formulation, characterization and tissue distribution of a novel pH-sensitive long-circulating liposome-based theranostic suitable for molecular imaging and drug delivery

Duan

Wei²,

Petryk³

et al. 2016

IJN

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Effects of ammonium chloride and chloroquine on endocytic uptake of liposomes by Kupffer cells in vitro

Cited by 48 publications

References 29 publications

Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection

Distinct effects of endosomal escape and inhibition of endosomal trafficking on gene delivery via electrotransfection

Lipid-based systems for the intracellular delivery of genetic drugs

Formulation, characterization and tissue distribution of a novel pH-sensitive long-circulating liposome-based theranostic suitable for molecular imaging and drug delivery

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