Effects of alternative electron acceptors on the activity and community structure of methane-producing and consuming microbes in the sediments of two shallow boreal lakes

Rissanen, Antti J.; Karvinen, Anu; Nykänen, Hannu; Peura, Sari; Tiirola, Marja; Mäki, Anita; Kankaala, Paula

doi:10.1093/femsec/fix078

Cited by 35 publications

(31 citation statements)

References 100 publications

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“…ANME-2d has previously been found the most abundant AOM clade in some lakes (Weber et al 2017;Rissanen et al 2017). We also observed the highest abundances of ANME-2d at the river mouthsite, while at the slightly more saline site D, ANME-2d was still present, but in much lower abundances ( Fig.…”

Section: Microbial Community Data In the Context Of Oxidation Rate Datasupporting

confidence: 78%

Influence of electron acceptor availability and microbial community structure on sedimentary methane oxidation in a boreal estuary

et al. 2020

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Methane is produced microbially in vast quantities in sediments throughout the world's oceans. However, anaerobic oxidation of methane (AOM) provides a near-quantitative sink for the produced methane and is primarily responsible for preventing methane emissions from the oceans to the atmosphere. AOM is a complex microbial process that involves several different microbial groups and metabolic pathways. The role of different electron acceptors in AOM has been studied for decades, yet large uncertainties remain, especially in terms of understanding the processes in natural settings. This study reports Keywords Baltic sea Á Methanotrophy Á Radiotracer incubation Á High throughput sequencing Á 16S rRNA gene

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Section: Microbial Community Data In the Context Of Oxidation Rate Datasupporting

confidence: 78%

Influence of electron acceptor availability and microbial community structure on sedimentary methane oxidation in a boreal estuary

et al. 2020

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“…Nevertheless, the relevance of AOM in reducing CH 4 emissions from freshwater lakes is still unclear and needs further research; e.g. Rissanen et al (2017) did not detect AOM coupled to any of the inorganic alternative EAs in the sediments of two shallow boreal lakes in Finland, while significant AOM was observed in 13 out of 14 study lake sediments in the temperate, arctic and tropical zone (Martinez-Cruz et al 2018).…”

Section: Introductionmentioning

confidence: 93%

CH4 oxidation in a boreal lake during the development of hypolimnetic hypoxia

et al. 2019

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Freshwater ecosystems represent a significant natural source of methane (CH 4). CH 4 produced through anaerobic decomposition of organic matter (OM) in lake sediment and water column can be either oxidized to carbon dioxide (CO 2) by methanotrophic microbes or emitted to the atmosphere. While the role of CH 4 oxidation as a CH 4 sink is widely accepted, neither the magnitude nor the drivers behind CH 4 oxidation are well constrained. In this study, we aimed to gain more specific insight into CH 4 oxidation in the water column of a seasonally stratified, typical boreal lake, particularly under hypoxic conditions. We used 13 CH 4 incubations to determine the active CH 4 oxidation sites and the potential CH 4 oxidation rates in the water column, and we measured environmental variables that could explain CH 4 oxidation in the water column. During hypolimnetic hypoxia, 91% of available CH 4 was oxidized in the active CH 4 oxidation zone, where the potential CH 4 oxidation rates gradually increased from the oxycline to the hypolimnion. Our results showed that in warm springs, which become more frequent, early thermal stratification with cold well-oxygenated hypolimnion delays the period of hypolimnetic hypoxia and limits CH 4 production. Thus, the delayed development of hypolimnetic hypoxia may partially counteract the expected increase in the lacustrine CH 4 emissions caused by the increasing organic carbon load from forested catchments.

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“…16S rRNA sequences were assigned taxonomies with a naïve Bayesian classifier (bootstrap cutoff value 75%) (Wang et al 2007), using the Silva database (Release 128), and sequences classified as archaea, chloroplast, mitochondria, and eukaryota were removed. Taxonomic classification of the functional genes took place similarly but with recently constructed databases for mcrA (Rissanen et al 2017) and pmoA (Dumont et al 2014).…”

Section: Dna-and Rna-based Amplicon Sequencing Analysesmentioning

confidence: 99%

Gammaproteobacterial methanotrophs dominate methanotrophy in aerobic and anaerobic layers of boreal lake waters

Rissanen¹,

Saarenheimo²,

Tiirola³

et al. 2018

Aquat. Microb. Ecol.

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Small oxygen-stratified humic lakes of the boreal zone are important sources of methane to the atmosphere. Although stable isotope profiling has indicated that a substantial part of methane is already oxidized in the anaerobic water layers in these lakes, the contributions of aerobic and anaerobic methanotrophs in the process are unknown. We used next-generation sequencing of mcrA and 16S rRNA genes to characterize the microbial communities in the water columns of 2 boreal lakes in Finland, Lake Alinen-Mustajärvi and Lake Mekkojärvi, and complemented this with a shotgun metagenomic analysis from Alinen-Mustajärvi and an analysis of pmoA genes and 16S rRNA, mcrA, and pmoA transcripts from Mekkojärvi. Furthermore, we tested the effect of various electron acceptors and light on methane oxidation ( 13 C-CH 4 labeling) in incubations of water samples collected from the lakes. Aerobic gammaproteobacterial methanotrophs (order Methylococcales) exclusively dominated the methanotrophic community both above and below the oxycline in the lakes. A novel lineage within Methylococcales, Candidatus Methyloumidiphilus alinensis, defined here for the first time, dominated in Alinen-Mustajärvi, while methanotrophs belonging to Methylobacter were more abundant in Mekkojärvi. Light enhanced methane oxidation in the anoxic water layer, while alternative electron acceptors (SO 4 2− , Fe 3+ , Mn 4+ , and anthraquinone-2, 6-disulfonate), except for NO 3 − , suppressed the process. Our results suggest that oxygenic photosynthesis potentially fuels methanotrophy below the aerobic water layers in methane-rich boreal lakes. Furthermore, incubation results, together with the detection of denitrification genes from metagenome-assembled genomes of gamma proteobacterial methanotrophs, imply that boreal lake methanotrophs may couple methane oxidation with NO x − reduction in hypoxic conditions.

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Effects of alternative electron acceptors on the activity and community structure of methane-producing and consuming microbes in the sediments of two shallow boreal lakes

Abstract: (2017). Effects of alternative electron acceptors on the activity and community structure of methane-producing and -consuming microbes in the sediments of two shallow boreal lakes. FEMS Microbiology Ecology, 93 (7)

Cited by 35 publications

References 100 publications

Influence of electron acceptor availability and microbial community structure on sedimentary methane oxidation in a boreal estuary

Influence of electron acceptor availability and microbial community structure on sedimentary methane oxidation in a boreal estuary

CH4 oxidation in a boreal lake during the development of hypolimnetic hypoxia

Gammaproteobacterial methanotrophs dominate methanotrophy in aerobic and anaerobic layers of boreal lake waters

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