Effects of alterations in hormonal status on ribosomes of rat uterus

Greenman, David L.; Kenney, Francis T.

doi:10.1016/0003-9861(64)90261-9

Cited by 45 publications

(12 citation statements)

References 15 publications

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“…The final MgCl2 concentration was adjusted to 10 mm. This is similar to that used by Campbell et al (1964) in a study of the effects of polyU on rat-liver microsomes and by Greenman & Kenney (1964) in their work on rat uterine ribosomes. The tubes were incubated in air at 370 and the reactions were initiated by the addition of the microsomal suspension.…”

Section: Methodssupporting

confidence: 73%

“…To study RNA polymerase (EC 2.7.7.6) directly, in vitro, it is necessary to prepare very clean preparations of nuclei (Gorski, 1964;Widnell & Tata, 1964). Considerable difficulties are encointered in the preparation of clean nuclei from solid tumours owing to the extreme resistance of the tumour matrix to conventional homogenization (see review by Dounce, 1963 Kenney (1964) reported that the incorporation of [14C]phenylalanine into uterine ribosomes was decreased after oophorectomy. Further, the ribosomal system from oophorectomized animals was more responsive to polyUt than uterine ribosomes from untreated control animals.…”

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confidence: 99%

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The effect of oophorectomy on the ribonucleic acid metabolism of a transplantable uterine tumour

Mainwaring¹,

Williams²

1965

Biochemical Journal

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1. The incorporation of [(14)C]phenylalanine and of [(3)H]uridine into the Guerin T8 uterine carcinoma has been examined. 2. The addition in vitro of polyuridylic acid caused an increase in the incorporation of labelled phenylalanine into tumour microsomes obtained from both oophorectomized and intact animals. 3. Oophorectomy of tumour-bearing animals decreased the specific activity due to the uptake in vivo of [(3)H]uridine into both the soluble and insoluble RNA of the tumour. 4. Actinomycin D caused a decrease in specific activity of RNA in the tumour in vivo, and puromycin inhibited uptake in vitro into tumour microsomes.

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Section: Methodssupporting

confidence: 73%

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confidence: 99%

The effect of oophorectomy on the ribonucleic acid metabolism of a transplantable uterine tumour

Mainwaring¹,

Williams²

1965

Biochemical Journal

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“…The largest proportion of the cellular R N A is ribosomal RNA, and a number of investigators have reported an increase in uterine ribosome forma-tion upon estrogen administration. Greenman and Kenney (2) found that the ribosome content of a uterine system capable of cell-free protein synthesis was decreased by ovariectomy and restored after the administration of estrogen to the ovariectomized animal. Moore and Hamilton (3) noted an increase in the synthesis of ribosomal R N A up to 24 hours after a single injection of estrogen into the ovariectomized animal, as indicated by the incorporation of tritiated uridine into ribosomal RNA, and by an increase in the cytoplasmic level of 78S ribosomes.…”

Section: Discussionmentioning

confidence: 99%

“…Recent biochemical studies have emphasized the role of estrogens in the promotion of uterine RNA and protein syntheses. Administration of estrogen to the ovariectomized animal (2)(3)(4) induces an increase in the number of uterine ribosomes, followed by an increase in the amount of cellular and extracellular protein. Smooth muscle cells do not usually contain an extensive rough endoplasmic reticulum, and, therefore, have not been considered to have, as an important function, the synthesis of protein for export.…”

Section: Introductionmentioning

confidence: 99%

Fine Structural Changes in Uterine Smooth Muscle and Fibroblasts in Response to Estrogen

Ross

Klebanoff

1967

The Journal of Cell Biology

136

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The fine structure of the estrogen-primed uterus was examined in two series of rats, with emphasis upon the alterations in smooth muscle cells and fibroblasts. The first series of animals were mature animals that were sacrificed at diestrus or estrus. The second series consisted of prepubertal rats (57-70 g) that received subcutaneous injections of estradiol-17 in 20 % alcohol. Four groups of animals received the hormone twice daily for 3 days for a total dose of 0.06, 0.6, 6.0, or 60.0 #g, respectively. An estrogenic response was observed in all groups as indicated by an increase in uterine weight. Control groups consisted of either untreated animals or animals receiving 20% alcohol. All animals were sacrificed on the 4th day. The fibroblasts and smooth muscle cells in the controls were similar to their counterparts in the mature animal in diestrus. They were small, contained relatively little rough endoplasmic reticulum, and the connective tissue cells appeared like fibrocytes. All of the estrogen-treated animals were similar in appearance and were comparable to their counterparts in the mature animal in estrus. Both the smooth muscle cells and the fibroblasts were increased in size, demonstrated a marked enlargement and dilation of ergastoplasmic cisternae, and contained increased numbers of attached and free cytoplasmic ribosomes. The presence of an extensive rough endoplasmic reticulum in the smooth muscle cells of the stimulated uterus is in marked contrast to the appearance of these cells in other tissues. These observations correlate with previous biochemical studies by other workers, in which estrogens have been shown to promote the synthesis of uterine RNA, collagen, and noncollagenous protein, and suggest that smooth muscle cells may participate in the synthesis of connective tissue proteins.

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“…The pathway and GO enrichment analyses for the DE genes showed that terms related to the ‘ribosome pathway’ were significant in our target list. It has long been known that the estrous cycle and estrogen signaling influences ribosome quantity and capacity of the uterus tissue (Greenman & Kenney ). Post‐puberty, the uterus is larger, and especially during the luteal phase it is much larger, than in pre‐puberty heifers.…”

Section: Discussionmentioning

confidence: 99%

Pre‐ and post‐puberty expression of genes and proteins in the uterus of Bos indicus heifers: the luteal phase effect post‐puberty

et al. 2018

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Progesterone signaling and uterine function are crucial in terms of pregnancy establishment. To investigate how the uterine tissue and its secretion changes in relation to puberty, we sampled tissue and uterine fluid from six pre- and six post-pubertal Brahman heifers. Post-pubertal heifers were sampled in the luteal phase. Gene expression of the uterine tissue was investigated with RNA-sequencing, whereas the uterine fluid was used for protein profiling with mass spectrometry. A total of 4034 genes were differentially expressed (DE) at a nominal P-value of 0.05, and 26 genes were significantly DE after Bonferroni correction (P < 3.1 × 10 ). We also identified 79 proteins (out of 230 proteins) that were DE (P < 1 × 10 ) in the uterine fluid. When we compared proteomics and transcriptome results, four DE proteins were identified as being encoded by DE genes: OVGP1, GRP, CAP1 and HBA. Except for CAP1, the other three had lower expression post-puberty. The function of these four genes hypothetically related to preparation of the uterus for a potential pregnancy is discussed in the context of puberty. All DE genes and proteins were also used in pathway and ontology enrichment analyses to investigate overall function. The DE genes were enriched for terms related to ribosomal activity. Transcription factors that were deemed key regulators of DE genes are also reported. Transcription factors ZNF567, ZNF775, RELA, PIAS2, LHX4, SOX2, MEF2C, ZNF354C, HMG20A, TCF7L2, ZNF420, HIC1, GTF3A and two novel genes had the highest regulatory impact factor scores. These data can help to understand how puberty influences uterine function.

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Effects of alterations in hormonal status on ribosomes of rat uterus

Cited by 45 publications

References 15 publications

The effect of oophorectomy on the ribonucleic acid metabolism of a transplantable uterine tumour

The effect of oophorectomy on the ribonucleic acid metabolism of a transplantable uterine tumour

Fine Structural Changes in Uterine Smooth Muscle and Fibroblasts in Response to Estrogen

Pre‐ and post‐puberty expression of genes and proteins in the uterus of Bos indicus heifers: the luteal phase effect post‐puberty

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