Effects of alpha-interferon on gamma-interferon production of peripheral blood mononuclear cells in hepatitis B virus carriers

Katayama, Kazuhiro; Hayashi, Norio; Takehara, Takumi; Towata, Takahiro; Kasahara, Akinori; Fusamoto, Hideyuki; Kamada, Takenobu

doi:10.1007/bf00920792

Cited by 8 publications

(2 citation statements)

References 28 publications

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“…HBV surface antigen (HBsAg), anti-HBV surface antibody (HBsAb) and anti-HCV antibody were measured by the microparticle enzyme immunoassay (MEIA) using the Abbott Axsym System instrument (Abbott Laboratories Ser vices Corporation, Taipei, Taiwan). LD was defined by cutoff > 40 U/L for ALT [14] .…”

Section: Methodsmentioning

confidence: 99%

Sonographic fatty liver and hepatitis B virus carrier status: Synergistic effect on liver damage in Taiwanese adults

Lin¹,

Hsiao²,

Chen³

2007

WJG

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Section: Methodsmentioning

confidence: 99%

Sonographic fatty liver and hepatitis B virus carrier status: Synergistic effect on liver damage in Taiwanese adults

Lin¹,

Hsiao²,

Chen³

2007

WJG

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“…Recently, several studies showed that the production of interferon-? (IFN-y) and monokines is closely associated with clearance of HBV-infected hepatocytes [Muto et al, 1988;Inoue et al, 1989;Daniels et al, 1990;Katayama et al, 1992;Takehara et al, 19921. Based on these findings, IFN-y or tumor necrosis factor-a (TNF-a) was administered to patients with type B chronic hepatitis in several studies.…”

Section: Introductionmentioning

confidence: 99%

Tumor necrosis factor‐α and interferon‐γ inhibit synergistically viral replication in hepatitis B virus‐replicating cells

Kawanishi

Hayashi

Katayama

et al. 1995

Journal of Medical Virology

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The effects of tumor necrosis factor-alpha and/or interferon-gamma on the replication of hepatitis B virus were examined using HB611 cells. These cells were derived from human hepatoblastoma cells, Huh6, by integrating hepatitis B virus DNA, and produce hepatitis B virus continuously. Each of the cytokines inhibited hepatitis B virus replication in the cells assessed as the amount of episomal hepatitis B virus DNA, without a decrease in cell viability. When the two cytokines were administered together, the inhibitory effect became greater. Incubation of the cells with 1,000 U/ml tumor necrosis factor-alpha decreased HBV DNA replicative intermediates by 55%, and that with 1,000 U/ml interferon-gamma decreased these by 51%. Furthermore, incubation with 1,000 U/ml tumor necrosis factor-alpha and 1,000 U/ml interferon-gamma in combination decreased HBV DNA replicative intermediates by 71%. In contrast, the amount of hepatitis B virus RNA and secretion of hepatitis B e antigen were not apparently reduced by the cytokines, and 2',5'-oligoadenylate synthetase activity was not detected in the supernatant. These results suggest that tumor necrosis factor-alpha and interferon-gamma inhibit hepatitis B virus replication by blocking some step in reverse transcription and that the 2',5'-oligoadenylate synthetase is not involved in the mechanism underlying the inhibition by these two cytokines.

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Alpha-interferon enhances gamma-interferon production of peripheral blood mononuclear cells pre-activated with phytohemagglutinin

et al. 1993

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The question of whether the state of T cell activation is responsible for gamma-interferon production in response to alpha-interferon was determined. When peripheral blood mononuclear cells were pre-incubated with phytohemagglutinin for 3 days, their gamma-interferon production was found to be augmented by alpha-interferon, accompanied by an increased proportion of DR-positive T cells. This did not occur with fresh cells. The effect was dose-dependent and inhibited by anti-alpha-interferon antibody. Therefore, alpha-interferon was considered to enhance the production of gamma-interferon of pre-activated peripheral blood mononuclear cells but not of resting ones. In conclusion, alpha-interferon augmented the production of gamma-interferon of peripheral blood mononuclear cells only when they had already been activated, suggesting a role of alpha-interferon as a modulator of the cellular immune response.

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Effects of alpha-interferon on gamma-interferon production of peripheral blood mononuclear cells in hepatitis B virus carriers

Cited by 8 publications

References 28 publications

Sonographic fatty liver and hepatitis B virus carrier status: Synergistic effect on liver damage in Taiwanese adults

Sonographic fatty liver and hepatitis B virus carrier status: Synergistic effect on liver damage in Taiwanese adults

Tumor necrosis factor‐α and interferon‐γ inhibit synergistically viral replication in hepatitis B virus‐replicating cells

Alpha-interferon enhances gamma-interferon production of peripheral blood mononuclear cells pre-activated with phytohemagglutinin

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