Effects of allyl sulfur compounds and garlic extract on the expression of Bcl-2, Bax, and p53 in non small cell lung cancer cell lines

Hong, Yong-Kil; Ham, Yoon-Ae; Choi, Ji-Hyung; Kim, Young Tae

doi:10.1038/emm.2000.22

Cited by 157 publications

(92 citation statements)

References 22 publications

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“…Moreover, garlic oil presented a prominent ability to induce cell apoptosis using staining with Annexin V-FITC/PI, confirming that the underling mechanism of anti-proliferation was mainly mediated by an induction of apoptosis, also proved by our further investigation of TEM and FCM. The above results were similar to the earlier report that garlic oil is effective in the reduction of anti-proliferative gene and modulation of apoptosis-associated cellular proteins in non small cell lung cancer cells (Hong et al, 2000).…”

Section: Discussionsupporting

confidence: 92%

Effects of Garlic Oil on Pancreatic Cancer Cells

Lan¹,

Sun²,

Liu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Section: Discussionsupporting

confidence: 92%

Effects of Garlic Oil on Pancreatic Cancer Cells

Lan¹,

Sun²,

Liu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…Using other cell lines, Al 2 S and Al 2 S 2 , in contrast to Al 2 S 3 , seem to cause only a slight decrease in the expression of Bcl-2 and a slight increase in Bax expression, a reflection of the fact that Al 2 S and Al 2 S 2 are only weak inducers of apoptosis (16). This notion has been confirmed in the present study.…”

Section: Discussionsupporting

confidence: 87%

Diallylpolysulfides induce growth arrest and apoptosis

Busch

Jacob²,

Ali³

et al. 2010

Int J Oncol

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Abstract. Garlic-derived organo sulphur compounds such as diallylsulfides provide a significant protection against carcinogenesis. Chemically synthesized, and highly pure diallylsulfides with a chain of 1-4 sulphur atoms, as well as a range of control compounds, were employed to investigate the influence of these agents on cell viability, cell cycle arrest and induction of apoptosis in HCT116 human colon cancer cells. Diallyltrisulfide, and even more efficiently diallyltetrasulfide treatment of HCT116 cells led to a reduced cell viability, cell cycle arrest and apoptosis. A similar activity was found for the propylanalogues, while mono-and disulfides were considerably less active. Initial calculations point toward the ability of triand tetrasulfides to form reactive oxygen species (ROS). Here, we found that the induction of apoptosis was indeed dependent on the redox-state of the cell, with anti-oxidants being able to prevent sulfide-induced apoptosis. Furthermore, using HCT116 cells which were either positive or negative for p53 revealed that p53 is clearly dispensable for induction of apoptosis. Growth arrest and induction of apoptosis is associated with a considerable reduction of the level of cdc25C. These results support the therapeutic potential of polysulfides and allow insight into the mechanisms based on the polysulfide biochemistry.

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“…Several studies have demonstrated the efficacy of garlic compounds as antitumorigenic agents. For example, DADS inhibits the in vitro growth of colon, lung, breast and gastric cancer cell lines, and leukemia cell lines [8][9][10][11][12], indicating that DADS may be a useful therapeutic tool in the prevention of environmentally induced cancers. We previously reported that DADS induced G2/M arrest and inhibited growth of MGC803 cells.…”

Section: Introductionmentioning

confidence: 99%

Erk is involved in the differentiation induced by diallyl disulfide in the human gastric cancer cell line MGC803

Ling¹,

Zhang²,

Qi³

et al. 2006

Cellular and Molecular Biology Letters

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Diallyl disulfide (DADS) is a major constituent of garlic. Previously, we found that DADS both inhibited proliferation in human gastric cancer cells in vitro and in vivo, and induced G2/M arrest. In this study, we investigated whether this differentiation effect was induced by DADS in human gastric cancer MGC803 cells, and whether it was related to an alteration in ERK activity. The results showed that the growth of MGC803 cells was inhibited by DADS. Cells treated with DADS displayed a lower nucleocytoplasmic ratio and tended to form gland and intercellular conjunction structures. The ConAmediated cell agglutination ratio and cells' ALP specific activity decreased. In MGC803 cells, dye transfer was limited to a few cells neighbouring the dyeinjected cell and to a depth of 1-2 layers beneath the scrape site. However, after treatment with DADS, the LY (Lucifer Yellow) was transferred to several cells immediately neighbouring the microinjected cell and to a depth of 2-4 cell layers from the scrape site. This indicated that DADS induced differentiation in MGC803 cells. Western blot analysis revealed that although DADS did not influence the quantity of ERK1/2 protein expressed, it did decrease its phosphorylation in a concentration-dependent manner, compared with the controls. At 30 mg·L -1 , DADS inhibited the activation of ERK1/2 in 15-30 min. These results suggested that the DADS-induced differentiation of MGC803 cells involved an alteration of the ERK1/2 signaling pathway.

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Effects of allyl sulfur compounds and garlic extract on the expression of Bcl-2, Bax, and p53 in non small cell lung cancer cell lines

Cited by 157 publications

References 22 publications

Effects of Garlic Oil on Pancreatic Cancer Cells

Effects of Garlic Oil on Pancreatic Cancer Cells

Diallylpolysulfides induce growth arrest and apoptosis

Erk is involved in the differentiation induced by diallyl disulfide in the human gastric cancer cell line MGC803

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