Effects of Allelochemicals, Soil Enzyme Activities, and Environmental Factors on Rhizosphere Soil Microbial Community of Stellera chamaejasme L. along a Growth-Coverage Gradient

Abstract

Allelochemicals released from the root of Stellera chamaejasme L. into rhizosphere soil are an important factor for its invasion of natural grasslands. The aim of this study is to explore the interactions among allelochemicals, soil physicochemical properties, soil enzyme activities, and the rhizosphere soil microbial communities of S. chamaejasme along a growth-coverage gradient. High-throughput sequencing was used to determine the microbial composition of the rhizosphere soil sample, and high-performance liquid chromatography was used to detect allelopathic substances. The main fungal phyla in the rhizosphere soil with a growth coverage of 0% was Basidiomycetes, and the other sample plots were Ascomycetes. Proteobacteria and Acidobacteria were the dominant bacterial phyla in all sites. RDA analysis showed that neochamaejasmin B, chamaechromone, and dihydrodaphnetin B were positively correlated with Ascomycota and Glomeromycota and negatively correlated with Basidiomycota. Neochamaejasmin B and chamaechromone were positively correlated with Proteobacteria and Actinobacteria and negatively correlated with Acidobacteria and Planctomycetes. Allelochemicals, soil physicochemical properties, and enzyme activity affected the composition and diversity of the rhizosphere soil microbial community to some extent. When the growth coverage of S. chamaejasme reached the primary stage, it had the greatest impact on soil physicochemical properties and enzyme activities.

Response of bacterial community characteristics in the rhizosphere soil of Stellera chamaejasme L. to its expansion on the Qinghai‐Tibet Plateau

“…In other words, an intermediate level of disturbance increases the diversity of microbial communities. PLS‐DA analysis further indicated the significant separation of rhizosphere bacterial community at diverse expansion levels, positively consistent with previous studies (Cheng et al, 2022). Plant factors have been proven to have a powerful explanation for changes in the soil bacterial community (Yu et al, 2019).…”

Endophytic bacterial community of Stellera chamaejasme L. and its role in improving host plants' competitiveness in grasslands

“…Proteobacteria was found to be the most dominant endophytic phylum in S. chamaejasme in both culturedependent and independent studies. Similarly, Jin et al (2018) and Cheng et al (2021) found that Proteobacteria was the dominant endophytic phylum in roots of S. chamaejasme from different elevation gradients. This is consistent with previous findings that Proteobacteria is widespread in the soil, marine, surface water and plant (Spain et al, 2009;Becerra-Castro et al, 2016;Timmermans et al, 2017;Dang et al, 2020).…”

An exploration of mechanism of high quality and yield of Gastrodia elata Bl. f. glauca by the isolation, identification and evaluation of Armillaria

“…Studies have shown that many orchid mycorrhizal fungi can promote nutrient absorption in the plant rhizosphere [29]. Meanwhile, they also affect changes in microbial community structures and their functions in rhizosphere soil [30][31][32] . However, the effect of symbiotic fungus Armillaria on the absorption of nutrition elements by G. elata Bl.…”