Effects of alkylphenols on CYP1A and CYP3A expression in first spawning Atlantic cod (Gadus morhua)

Hasselberg, Linda; Meier, Sonnich; Svardal, Asbjørn; Hegelund, Tove; Celander, Malin

doi:10.1016/j.aquatox.2003.12.007

Cited by 85 publications

(34 citation statements)

References 46 publications

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“…In the freshwater mussel Dreissena polymorpha, significant inhibition of EROD activity was observed after laboratory exposure to DDT (Binelli et al 2006). Similar results are provided by more numerous studies on fish, where exposure to nonylphenol (Vaccaro et al 2005), alkylphenols (Hasselberg et al 2004), organotin compounds (Fent et al 1998) and heavy metals (Oliveira et al 2004) caused reduced EROD activity. In the present study, the lowest EROD activity was observed in crabs from Marghera, in which the lowest AChE activity was also found.…”

Section: Discussionsupporting

confidence: 82%

Biomarker responses in the crab Carcinus aestuarii to assess environmental pollution in the Lagoon of Venice (Italy)

2009

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In the present study, three biomarkers, acetylcholinesterase (AChE) activity, vitellogenin (Vg)-like proteins, and ethoxyresorufin O-deethylase (EROD) activity, were evaluated in male Carcinus aestuarii crabs collected from four sites in the Lagoon of Venice, two in the northern lagoon area and two in the southern one. Results revealed differences among sites in relation to each specific biomarker. Hence, a suite of biomarkers can be used to discriminate sampling sites according to types of pollution, reflecting differing conditions of anthropogenic impact. Comparisons between the northern and southern lagoon areas suggest that the latter are in better condition for Vg-like protein level and AChE activity. The lower EROD activity recorded in crabs from the northern sites suggests a detrimental inhibitory effect of contaminants, rather than a lack of induction due to lower pollution levels.

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Section: Discussionsupporting

confidence: 82%

Biomarker responses in the crab Carcinus aestuarii to assess environmental pollution in the Lagoon of Venice (Italy)

2009

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“…It must also be considered that CYP1A catalytic activity varies between different xenobiotics and studies: variables such as species, sex, reproductive stage, compound, route of exposure, time of exposure, mixed exposure and/or dose may influence the observed effects. [59] A research study developed with Atlantic eel (Anguilla anguilla) showed a significant decrease in hepatic EROD activity after in vivo exposure to low doses (0.5 mg/L) of TNT (2,4,6-trinitrotoluene), but curiously no further decrease was observed when higher doses of this xenobiotic were assayed. [60] A similar decrease in EROD activity has been already reported in TNT-exposed rainbow trout (Onchorynchus mykiss), allowing us to hypothesize a potential role of TNT as a competitive inhibitor on EROD enzyme activity.…”

Section: Correlationsmentioning

confidence: 99%

Hepatic monooxygenase (CYP1A and CYP3A) and UDPGT enzymatic activities as biomarkers for long-term carbofuran exposure in tench (Tinca tincaL)

Hernández‐Moreno¹,

Soler-Rodríguez²,

Míguez-Santiyán³

et al. 2008

Journal of Environmental Science and Health, Part B

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The effect of a long-term exposure of tenchs to different concentrations (10 and 100 micro g/L) of the pesticide carbofuran has been evaluated. Microsomal hepatic cytochrome P450 subfamily 1A (CYP1A) and 3A (CYP3A) activities, as well as the phase II enzyme uridine diphospho-glucuronosyltransferase (UDPGT) activity were evaluated as adequate biomarkers of fish exposure to environmentally relevant concentrations of the pesticide carbofuran in freshwater ecosystems. A clear time-dependent inhibition of both CYP1A and UDPGT activities was observed in fish exposed to the highest dose of carbofuran with respect to controls, whereas in the case of CYP3A activity, values of exposed animals did not show a clear pattern of alteration during the experiment. The results of the present study demonstrated that hepatic CYP1A and UDPGT activities from tench could be considered as sensitive biomarkers for carbamate pesticides in polluted water, thus allowing future and ecologically relevant biomonitoring studies with this species.

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“…Similar to mammals, some drugs and endogenous compounds can induce CYP3A gene expression in reptiles, amphibians, and fish. Chemically-induced upregulation of CYP3A expression has been demonstrated in microsomes from alligator liver by phenobarbital and 3-methylcholanthrene [192], in microsomes from a toad epithelial cell line by dexamethasone and corticosterone [193], in Atlantic cod by alkylphenols [194], in rainbow trout and killifish by ketoconazole [195], in adult zebrafish liver by pregnenolone 16α-carbonitrile (but not clotrimazole or nifedipine) [185], and in larval zebrafish foregut by rifampin and dexamethasone [196]. The molecular mechanism of CYP3A induction in the species mentioned above has not been determined.…”

Section: Nr1i Subfamily Members In Non-mammalian Speciesmentioning

confidence: 99%

Evolution and Function of the NR1I Nuclear Hormone Receptor Subfamily (VDR, PXR, and CAR) with Respect to Metabolism of Xenobiotics and Endogenous Compounds

Reschly¹,

Krasowski²

2006

CDM

126

130

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The NR1I subfamily of nuclear hormone receptors includes the 1,25-(OH) 2 -vitamin D 3 receptor (VDR; NR1I1), pregnane X receptor (PXR; NR1I2), and constitutive androstane receptor (CAR; NR1I3). PXR and VDR are found in diverse vertebrates from fish to mammals while CAR is restricted to mammals. Current evidence suggests that the CAR gene arose from a duplication of an ancestral PXR gene, and that PXR and VDR arose from duplication of an ancestral gene, represented now by a single gene in the invertebrate Ciona intestinalis. Aside from the high-affinity effects of 1,25-(OH) 2 -vitamin D 3 on VDRs, the NR1I subfamily members are functionally united by the ability to bind potentially toxic endogenous compounds with low affinity and initiate changes in gene expression that lead to enhanced metabolism and elimination (e.g., induction of cytochrome P450 3A4 expression in humans). The detoxification role of VDR seems limited to sensing high concentrations of certain toxic bile salts, such as lithocholic acid, whereas PXR and CAR have the ability to recognize structurally diverse compounds. PXR and CAR show the highest degree of crossspecies variation in the ligand-binding domain of the entire vertebrate nuclear hormone receptor superfamily, suggesting adaptation to species-specific ligands. This review examines the insights that phylogenetic and experimental studies provide into the function of VDR, PXR, and CAR, and how the functions of these receptors have expanded to evolutionary advantage in humans and other animals.

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Effects of alkylphenols on CYP1A and CYP3A expression in first spawning Atlantic cod (Gadus morhua)

Cited by 85 publications

References 46 publications

Biomarker responses in the crab Carcinus aestuarii to assess environmental pollution in the Lagoon of Venice (Italy)

Biomarker responses in the crab Carcinus aestuarii to assess environmental pollution in the Lagoon of Venice (Italy)

Hepatic monooxygenase (CYP1A and CYP3A) and UDPGT enzymatic activities as biomarkers for long-term carbofuran exposure in tench (Tinca tincaL)

Evolution and Function of the NR1I Nuclear Hormone Receptor Subfamily (VDR, PXR, and CAR) with Respect to Metabolism of Xenobiotics and Endogenous Compounds

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