Effects of aligned and random fibers with different diameter on cell behaviors

Li, Xuyan; Wang, Xiaofeng; Yao, Dongshuang; Jiang, Jing; Guo, Xin; Gao, Yanhong; Li, Qian; Shen, Changyu

doi:10.1016/j.colsurfb.2018.07.045

Cited by 85 publications

(76 citation statements)

References 38 publications

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“…Generally, the diameter of fibers can affect the cellular adhesion contact point. 53 This may be because the morphology characteristics of the nanofibers are the same as that of the focal adhesion, and the amount has a high impact on cell proliferation and other behaviors. 54 Previous studies have demonstrated that vascular endothelial cells prefer smoother surfaces for adhesion and migration.…”

Section: Migration Assaymentioning

confidence: 99%

Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds

Chen¹,

Zhu²,

Fu³

et al. 2019

IJN

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Purpose: A promising vascular scaffold must possess satisfying mechanical properties, great hemocompatibility, and favorable tissue regeneration. Combining natural with synthetic materials is a popular method of creating/enhancing such scaffolds. However, the effect of additional modification on the materials requires further exploration. Materials and methods: We selected polycaprolactone (PCL), which has excellent mechanical properties and biocompatibility and can be combined with collagen. Electrospun fibers created using a PCL/collagen solution were used to fashion mixed nanofibers, while separate syringes of PCL and collagen were used to create separated nanofibers, resulting in different pore sizes. Mixed and separated nanofibers were cross-linked with glutaraldehyde (GA), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and genipin; hence, we named them as mixed GA, mixed EDC (ME), mixed genipin (MG), separated GA, separated EDC (SE), and separated genipin (SG). Results: Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction showed that cross-linking did not affect the main functional groups of fibers in all groups. ME, MG, SE, and SG met the requisite mechanical properties, and they also resisted collagenase degradation. In hemocompatibility assays, only ME and MG demonstrated ideal safety. Furthermore, ME and MG presented the greatest cytocompatibility. For vascular scaffolds, rapid endothelialization helps to prevent thrombosis. According to human umbilical vein endothelial cell migration on different nanofibers, ME and MG are also successful in promoting cell migration. Conclusion: ME and MG may be promising candidates for vascular tissue engineering. The study suggests that collagen cross-linked by EDC/N-hydroxysuccinimide or genipin facilitates endothelial cell regeneration, which could be of great benefit in tissue engineering of vascular scaffolds.

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Section: Migration Assaymentioning

confidence: 99%

Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds

Chen¹,

Zhu²,

Fu³

et al. 2019

IJN

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“…The porosity can be controlled via fiber size governed by electrospinning parameters, as well as the application of various collectors [10]. This technique allows the fabrication of both random and aligned fibers for different applications with a wide range of sizes from nano to micrometers, which influence different cell behavior on manufactured material [7,[11][12][13]. Aligned fibers fabricated via electrospinning were applied to build the ligament tissue based on the hierarchical structure [14].…”

Section: Introductionmentioning

confidence: 99%

Hierarchical Composite Meshes of Electrospun PS Microfibers with PA6 Nanofibers for Regenerative Medicine

et al. 2020

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One of the most frequently applied polymers in regenerative medicine is polystyrene (PS), which is commonly used as a flat surface and requires surface modifications for cell culture study. Here, hierarchical composite meshes were fabricated via electrospinning PS with nylon 6 (PA6) to obtain enhanced cell proliferation, development, and integration with nondegradable polymer fibers. The biomimetic approach of designed meshes was verified with a scanning electron microscope (SEM) and MTS assay up to 7 days of cell culture. In particular, adding PA6 nanofibers changes the fibroblast attachment to meshes and their development, which can be observed by cell flattening, filopodia formation, and spreading. The proposed single-step manufacturing of meshes controlled the surface properties and roughness of produced composites, allowing governing cell behavior. Within this study, we show the alternative engineering of nondegradable meshes without post-treatment steps, which can be used in various applications in regenerative medicine. supervision, U.S.; project administration, U.S.; funding acquisition, U.S. All authors have read and agreed to the published version of the manuscript.

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“…In that study, the enhanced cell spreading on enzyme-crosslinked materials was proven after excluding the other biophysical and biological factors by comparing the cell morphology on the substrates with a similar stiffness and arginine–glycine–aspartate (RGD) density. The strong actin filaments and high cell spreading area usually represent phenotypes for angiogenesis and vascularization [ 51 , 52 ]. Overall, though both Gel–HPA fibrous hydrogel and gelatin fibrous scaffolds showed excellent biocompatibility, Gel–HPA fibrous hydrogel crosslinked by HRP had the ability to enhance cell adhesion, cell spreading and cell proliferation, which are necessary for cell activities and tissue regeneration.…”

Section: Resultsmentioning

confidence: 99%

Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering

et al. 2020

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Soft tissue engineering has been seeking ways to mimic the natural extracellular microenvironment that allows cells to migrate and proliferate to regenerate new tissue. Therefore, the reconstruction of soft tissue requires a scaffold possessing the extracellular matrix (ECM)-mimicking fibrous structure and elastic property, which affect the cell functions and tissue regeneration. Herein, an effective method for fabricating nanofibrous hydrogel for soft tissue engineering is demonstrated using gelatin–hydroxyphenylpropionic acid (Gel–HPA) by electrospinning and enzymatic crosslinking. Gel–HPA fibrous hydrogel was prepared by crosslinking the electrospun fibers in ethanol-water solution with an optimized concentration of horseradish peroxidase (HRP) and H2O2. The prepared fibrous hydrogel held the soft and elastic mechanical property of hydrogels and the three-dimensional (3D) fibrous structure of electrospun fibers. It was proven that the hydrogel scaffolds were biocompatible, improving the cellular adhesion, spreading, and proliferation. Moreover, the fibrous hydrogel showed rapid biodegradability and promoted angiogenesis in vivo. Overall, this study represents a novel biomimetic approach to generate Gel–HPA fibrous hydrogel scaffolds which have excellent potential in soft tissue regeneration applications.

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Effects of aligned and random fibers with different diameter on cell behaviors

Cited by 85 publications

References 38 publications

<p>Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/<em>N-</em>hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds</p>

<p>Electrospun polycaprolactone/collagen nanofibers cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/<em>N-</em>hydroxysuccinimide and genipin facilitate endothelial cell regeneration and may be a promising candidate for vascular scaffolds</p>

Hierarchical Composite Meshes of Electrospun PS Microfibers with PA6 Nanofibers for Regenerative Medicine

Enzyme-Crosslinked Electrospun Fibrous Gelatin Hydrogel for Potential Soft Tissue Engineering

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