Effects of albendazole on Litomosoides chagasfilhoi (Nematoda: Filarioidea) females in vivo

Abstract

Gerbils (Meriones unguiculatus) experimentally infected with Litomosoides chagasfilhoi were treated with a single oral dose of 40 or 80 mg of albendazole, respectively. Observation of the microfilaremia after the treatment showed that both single oral doses of albendazole decreased the microfilaremia in L. chagasfilhoi infection. The body wall was composed of a cuticle, a hypodermis, and a muscular layer, and treated nematodes showed no morphological alterations. The ultrastructural alterations produced by treatment with 40 mg of albendazole included a higher number of membrane invaginations in the basal labyrinth of the uterine epithelium and the presence of myelin figures in this region. Inside the uterus, most embryos and microfilariae were disintegrated. The treatment with 80 mg of albendazole did not produce alterations in the uterine wall, and the number of vesicles near the microfilariae sheath was smaller than that observed in the untreated and in the 40-mg treatment groups. However, all the microfilariae observed in the uterus were extensively damaged with cytoplasmic vacuolization and cellular degeneration. No alterations in the intestinal cells were observed after treatment with 40 or 80 mg of albendazole. The present study contributes to the knowledge of albendazole's effects in filariids and demonstrates the potential embryotoxic and microfilaricidal consequences of this drug.

Profiling the macrofilaricidal effects of flubendazole on adult female Brugia malayi using RNAseq

“…Studies on the exposure of Toxocara canis and A. suum to FLBZ reported vacuolization of muscle, gonadal tissue, intestine and hypodermis (Hanser et al., 2003). FLBZ-induced damage to reproductive organs of filariae has also been reported (Howells and Delves, 1985, Cárdenas et al., 2010). …”

“…This study also found extensive damage to the hypodermis. Hypodermal damage is a common theme among the benzimidazoles including FLBZ (Lacey, 1988, Franz et al., 1990a, Kumar and Lai, 1998, Hanser et al., 2003, Shalaby et al., 2009, Cárdenas et al., 2010, O'Neill et al., 2015). Previous studies have shown that short-term exposure to FLBZ leads to extensive damage to B. malayi hypodermal tissue (O'Neill et al., 2015).…”

Ultrastructural changes in the advanced third larval stage of Gnathostoma binucleatum Almeyda-Artigas, 1991 (Nematoda: Spirurida) following albendazole and albendazole sulfoxide in vitro exposure

“…Scale bar 4.0 μm 2009), it is shown that this drug induces cuticular alterations, which are seen under the scanning electron microscope. In another recent study, ABZ was used in vitro, to see its effects on adult and microfilarial stages of Litomosoides chagasfilhoi; here, the main effect was inside the uterus, where the microfilaria were completely damaged, according with photomicrographs taken with transmission electron microscopy (Cárdenas et al 2010).…”

Perbandingan Aktivitas Antelmintik Albendazole dan Levamisole terhadap Ascaridia galli secara In Vitro

“…Pemakaian konsentrasi 10 mg/ml albendazole menurut Pal & Pandab (2010) menyebabkan paralisis dan kematian P. photuma selama 36 dan 84 menit masa inkubasi. Cárdenas et al (2010) membuktikan secara in vivo bahwa pemberian albendazole 40 mg dapat menurunkan 85.74% dan 80 mg 99.35% mikrofilaremia pada gerbil (Meriones unguiculatus) yang diinfeksi dengan Litomosoides chagasfilhoi. Pemberian albendazole dosis 400 mg dua kali satu hari selama tiga minggu dapat mereduksi level mikrofilaria 60% setelah 6 bulan (Klion, 2008).…”