Effects of AHLs inhibitors and exogenous AHLs on the stability and activity of Anammox granules at low temperatures

Abstract

This study investigated the relationship between acyl homoserine lactones (AHLs)based quorum sensing (QS) and the properties of Anammox granular sludge at low temperatures (11-23°C). Results indicated that adding different concentrations of AHLs inhibitors reduced the content of N-hexanoyl-dl-homoserine lactone (C6-HSL) and N-octanoyl-dl-homoserinelactone (C8-HSL) in Anammox granules on different degrees at different operation temperatures, which led to the deterioration of granules stability and activity. The important role of endogenous C6-HSL and C8-HSL signals in maintaining Anammox granular sludge stability and activity in low-temperature conditions was revealed. In addition, in the process of reducing operation temperatures, another type of AHL signal (N-(3-oxooctanoyl)-l-homoserine lactone, 3OC8-HSL) was released by Anammox granules. The effects of exogenous C8-HSL on the strength, average diameter, and density of Anammox granules were closely related to the operation temperature. When the operation temperature ranged from 11°C to 16°C, the stability of granules could be significantly improved by exogenous C8-HSL. In addition, the addition of C6-HSL and 3OC8-HSL promoted the activity of Anammox granules when the operation temperatures of the reactors were 11-23°C. This study proposed a novel approach to improve the properties of Anammox granules at low temperatures from the perspective of QS. • Practitioner points• Endogenous AHLs played an important role in maintaining the activity and stability of Anammox granules at 11-23°C. • Exogenous C8-HSL improved the granules stability at the low temperature of 11-16°C. • Exogenous C6-HSL or 3OC8-HSL promoted the granules activity at 11-23°C.• Supply a novel way to improve the Anammox granules performance at low temperatures.

Physiology of anammox adaptation to low temperatures and promising biomarkers: A review

“…On the other hand, N-acyl-homoserine lactones are a class of molecules involved in bacterial quorum sensing. The amendment of C6-and C8-homoserine lactones in anammox reactors increased the production of EPS and abundance of metabolism-associated genes (Liu et al, 2021;Zhang et al, 2021). These early and promising studies encourage further metabolomic studies into low-temperature anammox cultures.…”