Effects of Agitation, Aeration and Temperature on Production of a Novel Glycoprotein GP-1 by Streptomyces kanasenisi ZX01 and Scale-Up Based on Volumetric Oxygen Transfer Coefficient

Zhou, Yong; Han, Lirong; He, Hong‐Wei; Sang, Bu; Yu, Dai-Lin; Feng, Juntao; Zhang, Xing

doi:10.3390/molecules23010125

Cited by 93 publications

(63 citation statements)

References 42 publications

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“…The highest growth efficiency was observed with low agitation speed around 48.3%, whereas, the highest agitation speed decreased growth efficiency to 43.9% after 3 days, shown in Table 1 and Table S7. The decrease in the growth efficiency with increasing agitation speed may be related to the unbearable shear force, which caused the cell damage [50]. Or it may be due to the depression of the metabolic activity as reported by Vlaev et al [51].…”

Section: Microscopic Examination Of R Glutinis Cellular Shapes and Lmentioning

confidence: 83%

Manipulation of Culture Conditions: Tool for Correlating/Improving Lipid and Carotenoid Production by Rhodotorula glutinis

2020

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The coproduction of lipid and carotenoid by red yeasts in one cycle is more convenient and economical for the industrial sectors, while the kinetics correlation between both products under different culture conditions has been scarcely studied. This study is aiming to correlate the impact of different carbon sources, carbon to phosphorus ratio (C/P), temperature, aeration, pH, and metals on dry cell weight, lipid (GC and fluorescence microscope), and carotenoid (HPLC) production by Rhodotorula glutinis, and applying a novel feeding approach using a 5 L bioreactor to enhance carotenoid and unsaturated fatty acid production by R. glutinis. Whatever the culture condition is, the reversible correlation between lipid and carotenoid production was detected. Remarkably, when adding 0.1 mM BaCl2, cellular lipid was significantly increased 14% more than the control, with 79.3% unsaturated fatty acid (46% C18:2 and C18:3) and 50% γ-carotene, while adding 1 mM NiSO4, cellular carotenoid was enhanced around 53% than the control (torulene 88%) with 81% unsaturated fatty acid (61% oleic acid). Excitingly, 68.8 g/l biomass with 41% cellular lipid (79% unsaturated fatty acid) and 426 µgpigment/gdcw cellular carotenoid (29.3 mg/L) (71% torulene) were obtained, when the pH-temperature dual controlled process combined with metallo-sulfo-phospho-glucose feeding approach in the 5 L bioreactor during the accumulation phase was conducted. This is the first study on the kinetic correlation between lipid and carotenoid under different C/P ratio and the dual effect of different metals like NiSO4 on lipid and carotenoid production by red oleaginous yeasts, which in turn significant for enhancing the coproduction of lipid and carotenoid by R. glutinis.

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Section: Microscopic Examination Of R Glutinis Cellular Shapes and Lmentioning

confidence: 83%

Manipulation of Culture Conditions: Tool for Correlating/Improving Lipid and Carotenoid Production by Rhodotorula glutinis

2020

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“…Zhou et al [24] studied the effects of agitation and aeration on the production of a glycoprotein based on k L a and verified that k L a values increased as agitation speed and aeration rate increased in the bioreactor. At agitation speeds of 150 to 300 rpm and an aeration rate of 1 vvm, they reported similar k L a values (14.53-32.82 h −1 ) to this work.…”

Section: Relation Of the Volumetric Oxygen Transfermentioning

confidence: 99%

“…This calls for the optimization of the fermentation conditions. Other authors have studied the interactive influence of aeration and agitation rates on the volumetric oxygen transfer coefficient (k L a) to improve the production of several bioproducts, together with the negative impact of shear forces on microorganism and bioproduct production [20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Production of Biomass-Degrading Enzymes by Trichoderma reesei Using Liquid Hot Water-Pretreated Corncob in Different Conditions of Oxygen Transfer

et al. 2019

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Enzymatic hydrolysis accounts for 20% of the total cost in the conversion process of lignocellulosic biomass into bioethanol. Therefore, production of biomass-degrading enzymes by using lignocellulosic residue as a fermentation substrate may be an alternative to decrease the production costs. In this study, corncob (CC) has been pretreated by liquid hot water (LHW) at 200°C for 30 min and used as inducer source for production of biomass-degrading enzymes by Trichoderma reesei MUM 97.53. The pretreatment was used to increase the cellulose content and the accessibility to lignocellulosic material. Although the filamentous fungus secreted a broad range of cellulolytic and hemicellulolytic enzymes when grown on untreated CC, higher enzyme productions were obtained when cultured on LHW-pretreated CC in a 2-L stirred tank bioreactor (STB). Besides, the effects of aeration (2 and 4 vvm) and agitation (150 and 250 rpm) rates on enzyme production were studied by submerged fermentation in a batch STB and correlated with the volumetric oxygen transfer coefficient (k L a). Maximal cellulase, xylanase, and βxylosidase productions were found at 150 rpm and 4 vvm, while the highest β-glucosidase levels were obtained at 150 rpm and 2 vvm, that corresponded to k L a values of 32.50 h −1 and 16.41 h −1 , respectively. At higher agitation, a lower enzymatic production was observed probably due to the high shear stress in the fungal hyphae.

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“…The method requires the knowledge of k L a for oxygen under the pertinent growth conditions, which can be readily measured experimentally by the gassing‐out, gassing‐in method, as described previously . The k L a values from the published literature can be used when available for the matching conditions including agitation speed and pitch, flask geometry, and liquid levels in flask . Furthermore, the excellent correlation observed between the biomass accumulation and cumulative oxygen evolution provides an alternative, noninvasive method to estimate growth and will improve the extent of preliminary characterization of cyanobacteria in shake flasks.…”

Section: Resultsmentioning

confidence: 99%

A method to compute instantaneous oxygen evolution rates in cyanobacterial cultures grown in shake flasks

Sengupta

Wangikar

2020

Engineering Reports

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Cyanobacteria have been attracting attention for various biotechnological applications due to their ability to carry out oxygenic photosynthesis. The oxygen evolution rate (OER) of cyanobacterial cultures is a key physiological parameter, which is typically measured offline by using oxygen electrodes. In situ measurement of OER has been reported in a photobioreactor fitted with a dissolved oxygen (DO) probe. Here, we show that the OER can be estimated from online measurements of the DO levels by using sensors fitted in shake flasks, which are widely used for preliminary characterization of microbial strains. We study how the DO levels change under diurnal light regime and different growth conditions in two strains of Synechococcus elongatus, namely, PCC 7942 and PCC 11801, in shake flasks. We use the DO levels coupled with the knowledge of the overall gas-liquid mass transfer coefficient for oxygen (k L a) to compute the OER.For the growth conditions tested, the cumulative oxygen evolved in a day correlated with the increase in biomass suggesting that the calculated OER, in turn, might provide an estimate of the organism's growth rate. We believe that the method may be widely applicable for the growth characterization of organisms that perform oxygenic photosynthesis. K E Y W O R D Sabiotic factors, cyanobacteria, dissolved oxygen profile, oxygen evolution rates, PreSens technology, shake flaskThis is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Effects of Agitation, Aeration and Temperature on Production of a Novel Glycoprotein GP-1 by Streptomyces kanasenisi ZX01 and Scale-Up Based on Volumetric Oxygen Transfer Coefficient

Cited by 93 publications

References 42 publications

Manipulation of Culture Conditions: Tool for Correlating/Improving Lipid and Carotenoid Production by Rhodotorula glutinis

Manipulation of Culture Conditions: Tool for Correlating/Improving Lipid and Carotenoid Production by Rhodotorula glutinis

Production of Biomass-Degrading Enzymes by Trichoderma reesei Using Liquid Hot Water-Pretreated Corncob in Different Conditions of Oxygen Transfer

A method to compute instantaneous oxygen evolution rates in cyanobacterial cultures grown in shake flasks

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