Effects of ageing and exercise training on eNOS uncoupling in skeletal muscle resistance arterioles

Sindler, Amy L.; Delp, Michael D.; Reyes, Rafael; Wu, Guoyao; Muller‐Delp, Judy M.

doi:10.1113/jphysiol.2009.172221

Cited by 134 publications

(136 citation statements)

References 62 publications

(101 reference statements)

Supporting

Mentioning

124

Contrasting

Unclassified

Order By: Relevance

“…Trott and colleagues (46) recently reported that NAD(P)H-derived H 2 O 2 contributes to ACh-induced dilation in skeletal muscle resistance arteries of young rats but that NAD(P)H-derived O 2 Ϫ reduces endothelium-dependent vasodilation in arteries from old rats. In skeletal muscle arterioles, we have reported an age-related decrease in flow-induced, NO-mediated vasodilation that is accompanied by increased O 2 Ϫ production by uncoupled eNOS (41). Surprisingly, exercise training increased both aging dramatically increased NT protein levels in coronary arterioles from intact females.…”

mentioning

confidence: 89%

See 1 more Smart Citation

Aging and estrogen alter endothelial reactivity to reactive oxygen species in coronary arterioles

Kang

Chen

Reyes

et al. 2011

American Journal of Physiology-Heart and Circulatory Physiology

Self Cite

View full text Add to dashboard Cite

Kang LS, Chen B, Reyes RA, LeBlanc AJ, Teng B, Mustafa SJ, Muller-Delp JM. Aging and estrogen alter endothelial reactivity to reactive oxygen species in coronary arterioles. Am J Physiol Heart Circ Physiol 300: H2105-H2115, 2011. First published March 25, 2011 doi:10.1152/ajpheart.00349.2010.-Endothelium-dependent, nitric oxide (NO)-mediated vasodilation can be impaired by reactive oxygen species (ROS), and this deleterious effect of ROS on NO availability may increase with aging. Endothelial function declines rapidly after menopause, possibly because of loss of circulating estrogen and its antioxidant effects. The purpose of the current study was to determine the role of O 2 Ϫ and H2O2 in regulating flow-induced dilation in coronary arterioles of young (6-mo) and aged (24-mo) intact, ovariectomized (OVX), or OVX ϩ estrogen-treated (OVE) female Fischer 344 rats. Both aging and OVX reduced flowinduced NO production, whereas flow-induced H 2O2 production was not altered by age or estrogen status. Flow-induced vasodilation was evaluated before and after treatment with the superoxide dismutase (SOD) mimetic Tempol (100 M) or the H2O2 scavenger catalase (100 U/ml). Removal of H 2O2 with catalase reduced flow-induced dilation in all groups, whereas Tempol diminished vasodilation in intact and OVE, but not OVX, rats. Immunoblot analysis revealed elevated nitrotyrosine with aging and OVX. In young rats, OVX reduced SOD protein while OVE increased SOD in aged rats; catalase protein did not differ in any group. Collectively, these studies suggest that O 2 Ϫ and H2O2 are critical components of flow-induced vasodilation in coronary arterioles from female rats; however, a chronic deficiency of O 2 Ϫ buffering by SOD contributes to impaired flowinduced dilation with aging and loss of estrogen. Furthermore, these data indicate that estrogen replacement restores O 2 Ϫ homeostasis and flow-induced dilation of coronary arterioles, even at an advanced age. superoxide dismutase; hydrogen peroxide; ovariectomy; estrogen replacement

show abstract

mentioning

confidence: 89%

“…Ϫ signaling in response to intraluminal flow (41). Thus the relative source and contribution of NO-and O 2 Ϫ -derived species to endothelium-dependent vasodilation may be altered by physiological and pathophysiological stress.…”

Section: Enos-derived No and Omentioning

confidence: 99%

Aging and estrogen alter endothelial reactivity to reactive oxygen species in coronary arterioles

Kang

Chen

Reyes

et al. 2011

American Journal of Physiology-Heart and Circulatory Physiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…128 This result was confirmed in an aortocaval fistula model in the rat, wherein aortic blood flow is enhanced proximal but decreased distal to the fistula, 129 as well as in a rat exercise training model. 130 In addition, for circulating levels of ADMA, a beneficial effect of exercise training was documented. 131 Exercise, eNOS Activity, and Oxidative Stress.…”

Section: No Generation In the Endotheliummentioning

confidence: 99%

Cardiovascular Effects of Exercise Training

2010

View full text Add to dashboard Cite

“…Second, ETassociated reduction in OS, which has been confirmed in CKD patients, prevents ROS-mediated breakdown of NO (see OS below). Third, data from animal experiments suggest that ET can increase levels of the essential cofactor (6R)-5,6,7,8-tetrahydro-L-biopterin (BH4) (43), thereby preventing eNOS uncoupling and production of superoxide. In addition, ET in patients at CV risk has been shown to result in a significant decrease in circulating ADMA levels after 3 months (44), thus contributing to both an increase in eNOS activity and prevention of eNOS uncoupling.…”

Section: No Bioavailabilitymentioning

confidence: 99%

Vascular Effects of Exercise Training in CKD

Craenenbroeck

Kouidi

et al. 2014

Clinical Journal of the American Society of Nephrology

View full text Add to dashboard Cite

Cardiovascular disease remains the main cause of morbidity and mortality in patients with CKD, an observation that cannot be explained by the coexistence of traditional risk factors alone. Recently, other mechanisms, such as alterations in nitric oxide bioavailability, impaired endothelial repair mechanisms, inflammation, and oxidative stress (all characteristic in CKD), have gained much attention as mediators for the increased cardiovascular risk. Regular physical training is a valuable nonpharmacological intervention for primary and secondary prevention of cardiovascular disease. Likewise, the benefits of exercise training on exercise capacity and quality of life are increasingly recognized in patients with CKD. Furthermore, exercise training could also influence potential reversible mechanisms involved in atherosclerosis and arteriosclerosis. After discussing briefly the general concepts of vascular disease in CKD, this review provides an overview of the current evidence for the effects of exercise training at both clinical and preclinical levels. It concludes with some practical considerations on exercise training in this specific patient group.

show abstract

Effects of ageing and exercise training on eNOS uncoupling in skeletal muscle resistance arterioles

Abstract: Reduced availability of tetrahydrobiopterin (BH

Cited by 134 publications

References 62 publications

Aging and estrogen alter endothelial reactivity to reactive oxygen species in coronary arterioles

Aging and estrogen alter endothelial reactivity to reactive oxygen species in coronary arterioles

Cardiovascular Effects of Exercise Training

Vascular Effects of Exercise Training in CKD

Contact Info

Product

Resources

About