Effects of age, body weight, body condition and scrotal circumference on sperm abnormalities of bulls used for artificial insemination (AI) programme in Bangladesh

Abstract

Impacts of age, body weight, body conditions, and scrotal circumference on sperm abnormalities of bulls used for artificial insemination (AI) in Bangladesh have been studied. During 1997During -2002During , 1390 ejaculates of semen from 71 bulls from Central Cattle Breeding Station and Dairy Farm, Savar, Dhaka, Rajshahi Dairy and Cattle Improvement Farm, Rajabarihat, and District Artificial Insemination Centre, Rajshahi, were collected and examined. Total head abnormalities, free loose head, mid-piece, tail abnormalities, proximal and distal cytoplamic droplets, total tail abnormalities and total sperm abnormalities were recorded. Age of the bulls had significant effect on sperm abnormalities (P<0.01) except for proximal cytoplasmic droplet. Similarly, body weight had significant effect (P<0.05) except for the total head abnormalities, while body conditions had no significant effect on mid-piece, total tail abnormalities and total sperm abnormalities except for the total head abnormalities. Scrotal circumference had significant effect (P<0.05) on all the sperm abnormalities. The present results demonstrate that the middle (8 to <10 yrs) and older (>10 yrs) bulls having body weight of <450 kg, very good body condition and 36 to <38 cm scrotal circumference had the lowest sperm abnormalities. The need for semen examination for morphological abnormalities of spermatozoa in bulls to be used for AI programme is strongly implicated by this study.

Biological Integrity of Bison Epididymal Sperm Under Cryoconservation and Long Storage

“…The cause of idiopathic infertility may be a high index of DNA fragmentation in chromatin [57][58][59][60][61]. The DNA fragmentation in spermatozoa also depends on numerous biotic and abiotic factors [62][63][64][65][66][67][68][69].…”

Effect of Body Condition Score and PCR-RFLP Polymorphism of Prolactin Gene on Semen Characteristics of Buffalo Bulls (BubalusBubalis)

“…Similarly, Sarder [34] pointed that, there were no significant effects of body condition score of bulls on some semen characteristics. Meanwhile, Addass [35] reported that, there were significant effects of body condition score of bulls on sperm production potential and scrotal circumference.…”

Effect of pre-freeze semen quality, extender and cryoprotectant on the post-thaw quality of Asian elephant (Elephas maximus indicus) semen

“…Sperm morphology was evaluated using a protocol developed by Sarder (2008). Smears of fresh semen were air-dried and fixed in absolute alcohol for 3 min and then stained with William's stain for 5 min.…”

“…Stock solution III consisted of 10 ml stock solution I and 170 ml of 5% phenol. 25 ml of Stock solution II and 50 ml of stock solution III were mixed to produce the final staining solution, which was filtered before use (Sarder, 2008). After staining, the slides were washed in running tap water, dried and examined using bright field light microscopy at a magnification of x1000.…”

“…Following freezing and thawing, the DNA fragmentation index, and the dynamics of DNA fragmentation were also evaluated, using the sperm chromatin dispersion test. As sperm DNA stability tends to be disturbed during or after cryopreservation, especially in terms of the dynamics of sperm DNA fragmentation (SDF) 2008), SDF is considered to be a very informative parameter for assessing post-thaw semen quality. The sperm chromatin dispersion test involves an uncomplicated and inexpensive protocol for SDF (de la Torre et al, 2007) such that this test is appropriate for use with wild animal species.…”