Effects of Age and Nutrition on Expression of CD25, CD44, and l-Selectin (CD62L) on T-cells from Neonatal Calves

Abstract

Effects of the plane of nutrition and age on the proliferation and activation of lymphocyte subsets from milk replacer-fed calves were investigated in vitro. Holstein calves were fed a standard (0.45 kg/d of a 20% crude protein, 20% fat milk replacer, n = 4) or intensified (1.14 kg/d of a 28% crude protein, 20% fat milk replacer, n = 4) diet from 1 to 8 wk of age. Average daily weight gain of intensified-diet (0.66 kg/d) calves was greater than that of standard-diet (0.27 kg/d) calves. Relative to the pokeweed mitogen-induced responses of CD4(+) cells from steers (5 to 6 mo of age), CD4(+) cells from 1-wk-old calves showed decreased proliferative activity, delayed increase in CD25 expression, and no demonstrable increase in CD44 expression or decrease in CD62L expression. Calf CD8(+) and gammadeltaT-cell receptor(+) cells, unlike T-cells from the older animals, did not demonstrate decreased expression of CD62L after stimulation with mitogen. The increased expression of CD44 by mitogen-stimulated gammadeltaT-cell receptor(+) cells from older animals was not seen in gammadeltaT-cell receptor(+) cells from 1-wk-old calves. At wk 8 of age, mitogen-induced proliferation and expression of activation antigens by T-cells from standard-fed calves were similar to responses of T-cells from steers indicating rapid maturation of T-cell function during the neonatal period. Feeding calves an intensified milk replacer was associated with decreased proliferation of mitogen-stimulated CD4(+), CD8(+), and gammadeltaT-cell receptor(+) cells; decreased CD25 expression by mitogen-stimulated CD4(+) and CD8(+) cells; and decreased CD44 expression by mitogen-stimulated CD8(+) cells. These results indicate that the functional capacity of the calf's T-cell population becomes more adult-like during the first weeks of life and suggest that nutrition modulates T-cell function during this period of immune maturation.

Selective effects of Lactobacillus casei Shirota on T cell activation, natural killer cell activity and cytokine production

“…CD25 is expressed on activated T cells, B cells and monocytes. Formation of the high‐affinity IL‐2 receptor allows T cell proliferation and differentiation to be driven by IL‐2 [25]. Previous studies have shown overall agreement with CD25 or CD69 and proliferation assays when a recall specific antigen was used [24].…”

The stress of weaning influences serum levels of acute-phase proteins, iron-binding proteins, inflammatory cytokines, cortisol, and leukocyte subsets in Holstein calves

“…The α-chain of the IL-2 receptor, CD25, is expressed on activated T-cells, B-cells, and monocytes. Formation of the high-affinity IL-2 receptor allows T-cell proliferation and differentiation to be driven by IL-2 [9]. Although decreased IL-2 receptor expression is not direct evidence of immune attenuation, this result indicated that stress-associated metabolites such as cortisol might influence the proliferation or differentiation of immune cells.…”

Comparison of the peripheral blood leukocyte population between Japanese Black and Holstein calves

“…IL-2 is an important cytokine to activate T cells, and the IL-2 mRNA expression in stimulated PBMCs of newborn calves was lower than that of adult cows (Yamanaka et al 2003). CD25 is IL-2Ra, which is expressed on activated lymphocytes (Foote et al 2005). The CD25 expression on PBMCs from newborn calves was enhanced by several proinflammatory cytokine stimulations (Yamanaka et al 2003).…”