Effects of age and androgens upon functional vasoactive intestinal peptide receptors in rat prostatic epithelial cells

Carmena, Marı́a J.; Sancho, J.I.; Prieto, Juan-Carlos

doi:10.1016/0167-4889(86)90234-x

Cited by 13 publications

(4 citation statements)

References 21 publications

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“…The presence of these subepithelial nerves in the ampular acini suggests a role for the VIP in the regulation of their secretory activity (Juarranz et al, 2001; Ventura et al, 2002). This agrees with the detection of vipergic receptors in the epithelium of rat prostate (Carmena et al, 1985; Carmena et al, 1986a; Carmena et al, 1986b; Carmena et al, 1988).…”

Section: Discussionsupporting

confidence: 91%

Stereological Quantification of Nerve Fibers Immunoreactive to PGP 9.5, NPY, and VIP in Rat Prostate During Postnatal Development

Rodríguez

Pozuelo

Martı́n³

et al. 2005

Journal of Andrology

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This work was undertaken to study prostate innervation during the postnatal development of rats. It deals with the quantification of nervous fibers throughout all the regions of the rat prostate during the postnatal development using a general marker for nervous tissue, protein gene product 9.5, and 2 neuropeptides (NPY and VIP). Forty male Wistar rats (prepubertals, pubertals, young, and aged adults) were studied for immunohistochemistry of protein gene product (PGP 9.5), neuropeptide Y (NPY), and vasoactive intestinal polypeptide (VIP). They were also evaluated for length density of nerve fibers (L(V) PGP 9.5, L(V) NPY, L(V) VIP). Nerve fibers immunoreactive to the 3 antigens studied were detected in all the groups and in all the prostate zones. Periductal L(V) NPY evidenced a significant increase in the pubertal group, maintained throughout adult life. Periductal L(V) VIP showed a significant increase in young adults. The length densities of VIP and NPY fibers were significantly higher in periductal and ampular locations in comparison with dorsal and ventral sites. It can be concluded that the relative amount of nerve fibers in rat prostate, detected by PGP 9.5, does not change during postnatal development. There were significant changes in NPY and VIP fibers, showing an increase in periurethral ducts at puberty. The abundance of peptidergic innervation around the excretory ducts is related to their contractility. The development of innervation of periurethral ducts is regulated by androgens.

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Section: Discussionsupporting

confidence: 91%

Stereological Quantification of Nerve Fibers Immunoreactive to PGP 9.5, NPY, and VIP in Rat Prostate During Postnatal Development

Rodríguez

Pozuelo

Martı́n³

et al. 2005

Journal of Andrology

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“…Values lower than 200 cells were scored as negative. The selective agonists for VPAC 1 and VPAC 2 receptors, [K 15 , R 16 , L 27 ] VIP (1-7)/GFR (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27) and RO 25-1553, respectively, was a gift from Patrick Robberecht (Université Libre de Bruxelles, Belgium).…”

Section: Detection Of Ne Morphology In Lncap Cellsmentioning

confidence: 99%

“…In human and rat prostate, VIP/ PACAP receptors are mainly VPAC 1 receptors [5,12], and the same occurs in the prostate cancer cell lines LNCaP and PC-3 [8,10]. Moreover, the expression of VIP receptors in rat prostate increases during puberty, a period with high rates of cell proliferation and differentiation [13,14]. VIP receptors are mainly coupled to adenylate cyclase as a signal transduction pathway in normal and cancer prostate cells [7][8][9][10]12,15].…”

Section: Introductionmentioning

confidence: 99%

Vasoactive intestinal peptide induces neuroendocrine differentiation in the LNCaP prostate cancer cell line through PKA, ERK, and PI3K

et al. 2004

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“…Nerve-associated VIP immunoreactivity has been demonstrated in the prostate of a number of animal species, but the rat prostate has not been evaluated as yet [31]. Isolated rat prostatic epithelial cells, however, have been shown to display androgen-dependent VIP receptors on their surfaces [32]. In this study, VIP 100 pg/kg IV resulted in no stimulation of EGF secretion.…”

Section: Rat Prostatic Egf Secretionmentioning

confidence: 55%

Exocrine secretion of epidermal growth factor by the rat prostate: Effect of adrenergic agents, cholinergic agents, and vasoactive intestinal peptide

Jacobs

Story

1988

The Prostate

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Perfusion of the rat prostatic urethra in vivo provided a means of collecting the rat prostatic secretory product. The secretion of the protein epidermal growth factor (EGF) was investigated by radioimmunoassay (RIA). Baseline secretion of EGF into prostatic fluid was less than .03 +/- .004 SEM ng/min. The alpha-adrenergic agonist phenylephrine caused an increase in EGF to 3.6 +/- .4 SEM ng per 30-min period. The stimulation was blocked completely by prazosin and only partially by yohimbine, indicating primarily alpha 1 control. One mg/kg IV phenylephrine produced a maximal response. The beta-adrenergic agonist isoproterenol caused no increased secretion of EGF. The cholinergic agonist pilocarpine stimulated EGF secretion to 3.2 +/- .6 SEM ng per 30-min period. Atropine blocked the cholinergic stimulation. The combination of phenylephrine and pilocarpine did not result in greater stimulation than either agent alone. Vasoactive intestinal peptide (VIP) did not stimulate EGF secretion, nor did it augment either pilocarpine or phenylephrine-stimulated secretion. EGF secretion into rat prostatic fluid is under both alpha 1-adrenergic and cholinergic control.

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Effects of age and androgens upon functional vasoactive intestinal peptide receptors in rat prostatic epithelial cells

Cited by 13 publications

References 21 publications

Stereological Quantification of Nerve Fibers Immunoreactive to PGP 9.5, NPY, and VIP in Rat Prostate During Postnatal Development

Stereological Quantification of Nerve Fibers Immunoreactive to PGP 9.5, NPY, and VIP in Rat Prostate During Postnatal Development

Vasoactive intestinal peptide induces neuroendocrine differentiation in the LNCaP prostate cancer cell line through PKA, ERK, and PI3K

Exocrine secretion of epidermal growth factor by the rat prostate: Effect of adrenergic agents, cholinergic agents, and vasoactive intestinal peptide

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