Effects of Agaricus blazei Murill Polysaccharide on Cadmium Poisoning on the MDA5 Signaling Pathway and Antioxidant Function of Chicken Peripheral Blood Lymphocytes

Lv, Ai; Ge, Ming; Hu, Xuequan; Liu, Wenjing; Li, Guangxing; Zhang, Ruili

doi:10.1007/s12011-017-1012-4

Cited by 11 publications

(8 citation statements)

References 46 publications

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“…Zhang et al observed that Sargassum polysaccharide inhibited oxidative stress induced by infectious bursa disease virus in bursal lymphocytes of chickens [50]. Lv et al found that a polysaccharide from Agaricus blazei Murill had antioxidant effect in chicken peripheral blood lymphocytes treated with cadmium [51]. In this study, Rg1 significantly reduced oxidative stress-induced apoptosis and damage of chicken lymphocytes.…”

Section: Discussionmentioning

confidence: 50%

Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes

Wang

et al. 2019

Oxidative Medicine and Cellular Longevity

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Previous investigation showed that ginsenoside Rg1 (Rg1) extracted from Panax ginseng C.A. Mey has antioxidative effect on oxidative stress in chickens. The present study was designed to investigate the protective effects of Rg1 on chicken lymphocytes against hydrogen peroxide-induced oxidative stress and the potential mechanisms. Cell viability, apoptotic cells, malondialdehyde, activity of superoxide dismutase, mitochondrial membrane potential, and [Ca2+]i concentration were measured, and transcriptome analysis and quantitative real-time polymerase chain reaction were used to investigate the effect of Rg1 on gene expression of the cells. The results showed that treatment of lymphocytes with H2O2 induced oxidative stress and apoptosis. However, pretreatment of the cells with Rg1 dramatically enhanced cell viability, reduced apoptotic cells, and decreased oxidative stress induced by H2O2. In addition, Rg1 reduced these H2O2-dependent decreases in mitochondrial membrane potential and reversed [Ca2+]i overload. Transcriptome analysis showed that 323 genes were downregulated and 105 genes were upregulated in Rg1-treated cells. The differentially expressed genes were involved in Toll-like receptors, peroxisome proliferator-activated receptor signaling pathway, and cytokine-cytokine receptor interaction. The present study indicated that Rg1 may act as an antioxidative agent to protect cell damage caused by oxidative stress via regulating expression of genes such as RELT, EDA2R, and TLR4.

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Section: Discussionmentioning

confidence: 50%

Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes

Wang

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…Ethical treatment of animals used in this study was approved by the Animal Welfare Committee protocol (#NEAU-2013-02-0252-11) at Northeast Agricultural University (Harbin, China) Anticoagulant blood samples were dissected from 15-day-old Hyline egg-laying chickens (Harbin, China), and carefully placed in a tube containing lymphocyte in a separation medium (Tian Jin Hao Yang Biological Manufacture Co. Ltd., Tianjin, China), and the specific extraction method of chicken PBLs was routine procedure in our laboratory [ 12 ]. The acquired cells were cultured in a 37 °C 5% CO 2 incubator for 24 h. The APS were extracted from Astragalus by the Sevag method according to our previous study [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…The protein samples were separated using 8% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred to nitrocellulose membrane. The membrane was blocked using skim milk (5%) and then incubated with the primary antibody of MDA5 [ 12 ] (1:1000) at 4 °C overnight, followed by incubation with horseradish peroxidase-conjugated goat anti-mouse immunoglobulin (Ig)G (1:5000, Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) for 1 h after washing three times. At the same time, the membrane was incubated with the β-actin antibody (1:1000, Beijing Zhong Shan-Golden Bridge Biological Technology Co., Ltd., Beijing, China) followed by incubation with horseradish peroxidase-conjugated goat anti-mouse IgG (1:5000).…”

Section: Methodsmentioning

confidence: 99%

“…In the RLR family, MDA5 recognizes viral RNAs in the cytoplasm through C-terminal DExD/H box RNA helicase, and the two CARDs function through CARD-CARD interacts with interferon promoter-stimulating factor 1 ( IPS-1 ), leading to the activation of interferon regulatory factor 3 and NF-κB [ 11 ], which finally induces type I interferon ( IFN-I ) production and triggers a series of inflammatory reactions such as TNF-α , IL-1β , and IL-6 . The preliminary study created a chicken-based model for exposure to Cd in vivo, which showed that the expression of MDA5 and its signaling pathway molecule were enhanced in the model group [ 12 ]. These results suggest that the MDA5 signaling pathway may be involved in the injury process induced by Cd.…”

Section: Introductionmentioning

confidence: 99%

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Astragalus Polysaccharide Protect against Cadmium-Induced Cytotoxicity through the MDA5/NF-κB Pathway in Chicken Peripheral Blood Lymphocytes

Xie

et al. 2017

Molecules

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Cadmium (Cd) is a known environmental pollutant that is associated with inflammation, oxidative stress, and cell apoptosis. Astragalus polysaccharide (APS) is a major component of Astragalus membranaceus, a vital qi-reinforcing herb medicine with favorable immuneregulation properties. To study the effect of APS on the inhibition of the cadmium-induced injury of peripheral blood lymphocytes (PBLs) in chickens through the MDA5/NF-κB signaling pathway, PLBs acquired from 15-day-old chickens were divided into control group, Cd group, APS + Cd group, anti-MDA5 mAb + Cd group, BAY 11-7082 (a nuclear factor kappa-light chain-enhancer of activated B cells [NF-κB] inhibitor) +Cd group, APS group, anti-MDA5 mAb group, and BAY 11-7082 group. The transcription levels of melanoma differentiation-associated gene 5 (MDA5), interferon promoter-stimulating factor 1 (IPS-1), NF-κB, and inflammatory factors tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 were measured by quantitative real-time PCR. MDA5 protein expression was measured by western blotting. Levels of malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) were measured by corresponding antioxidant kit. The morphological change of PBLs was measured by transmission electron microscopy. The results showed that Cd significantly increased the expression of MDA5, IPS-1, NF-κB, and their downstream cytokines, IL-1β and TNF-α, IL-6 in PLBs. In addition, a high level of MDA was observed in the Cd treatment group; the activities of GSH-Px and SOD were significantly lower in the Cd treatment group than those in controls (p < 0.05). Ultrastructural changes of PBLs showed that Cd promoted autophagy, apoptosis, and necrosis in PBLs. However, APS can efficiently improve Cd-induced cell damage by decreasing the activation of the MDA5 signaling pathway. The effect is consistent with that of anti-MDA5 mAb or/and BAY. The results indicated that APS inhibited Cd-induced cytotoxicity through the regulation of MDA5/NF-κB signaling.

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“…Agaricus blazei Murill (AbM), known in China as jisongrong, is widely planted for its value as a food source and for its potential application in healthy protection [19,20] . The polysaccharides extracted from AbM exhibited various biological activities, such as regulating cellular immunity and providing antioxidative, antiatherosclerotic, antitumor, anti‐infective and anti‐inflammatory effects [27–31] . The activity of AbM polysaccharides is guided by substances such as the branched β‐1,3‐/1,6‐glucans and proteoglycans [32–36] .…”

Section: Introductionmentioning

confidence: 99%

Chemically Sulfated Polysaccharides from Agaricus blazei Murill: Synthesis, Characterization and Anti‐HIV Activity

Zhao¹,

Tian

Wang³

et al. 2021

Chemistry & Biodiversity

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AIDS, caused by HIV‐1, is one of the most dangerous infectious diseases in the world. Therefore, it is necessary to develop new drugs with more potent bioactivities, less toxicity and higher tolerability for controlling the viral load, particularly by using the raw materials that are widely available. Agaricus blazei Murill (AbM), known in China as jisongrong, is of great importance as a food source and as a health‐promoting supplement for immunomodulation. The polysaccharides of AbM exhibit various biological activities, such as regulating cellular immunity and providing anti‐oxidative, anti‐infective, and anti‐inflammatory effects. At present, to our knowledge, no report has explored the chemically sulfated and anti‐HIV‐1 activity of AbM polysaccharides. Herein, the sulfated AbM polysaccharides with different sulfur contents were prepared by the chlorosulfonic acid‐pyridine method. The characteristics of sulfated derivatives were established by the determination of the sulfur content, the relative molecular weight, and the Fourier‐transform infrared spectroscopy. The anti‐HIV activities of the sulfated AbM polysaccharides were evaluated by CCK‐8 and the single‐cycle pseudovirus infection (TZM‐bl) assay. The sulfated AbM polysaccharides had strong antiviral properties, and the half‐maximal inhibitory concentrations approached that of the positive control, azidothymidine. Sulfated modification of AbM polysaccharides can increase their anti‐HIV pharmacological activity, which makes them promising alternative candidates as bioactive macromolecules for biomedical applications in HIV/AIDS.

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Effects of Agaricus blazei Murill Polysaccharide on Cadmium Poisoning on the MDA5 Signaling Pathway and Antioxidant Function of Chicken Peripheral Blood Lymphocytes

Cited by 11 publications

References 46 publications

Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes

Protective Effect of Ginsenoside Rg1 on Oxidative Damage Induced by Hydrogen Peroxide in Chicken Splenic Lymphocytes

Astragalus Polysaccharide Protect against Cadmium-Induced Cytotoxicity through the MDA5/NF-κB Pathway in Chicken Peripheral Blood Lymphocytes

Chemically Sulfated Polysaccharides from Agaricus blazei Murill: Synthesis, Characterization and Anti‐HIV Activity

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