Effects of advanced maternal age are abrogated in 122 patients undergoing transfer of embryos with euploid microarray screening results at cleavage stage

Beltsos, Angie; Potter, Daniel; Bush, M.; Givens, C.; Smotrich, David B.

doi:10.1016/j.fertnstert.2010.07.310

Cited by 5 publications

(4 citation statements)

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“…This study shows vastly improved outcomes even when including cycles with no euploid embryos to transfer. The reduction in miscarriages suggested in this study is consistent with other retrospective studies that have shown a decrease in miscarriage rates in patients with recurrent pregnancy loss treated with CCS [14,22,27]. This study does have several limitations.…”

Section: Discussionsupporting

confidence: 88%

Preimplantation Genetic Screening (PGS) with Comparative Genomic Hybridization (CGH) following day 3 single cell blastomere biopsy markedly improves IVF outcomes while lowering multiple pregnancies and miscarriages

Keltz

Vega

Sirota

et al. 2013

J Assist Reprod Genet

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Purpose To determine benefits of cleavage-stage preimplantation genetic screening (PGS) by array comparative genomic hybridization (CGH). Methods A retrospective case-control study was performed at a tertiary care university-affiliated medical center. Implantation rate was looked at as a primary outcome. Secondary outcomes included clinical and ongoing pregnancy rates, as well as multiple pregnancy and miscarriage rates. Thirty five patients underwent 39 fresh cycles with PGS by aCGH and 311 similar patients underwent 394 invitro fertilization cycles. Result(s) The implantation rate in the CGH group doubled when compared to the control group (52.63 % vs. 19.15 %, p=<0.001), clinical pregnancy rate was higher (69.23 % vs. 43.91 %, p=0.0002), ongoing pregnancy rate almost doubled (61.54 % vs. 32.49 %, p=<0.0001), multiple pregnancy rate decreased (8.33 % vs. 34.38 %, p=0.0082) and miscarriage rate trended lower (11.11 % vs. 26.01 %, p=0.13). Conclusion Cleavage stage PGS with CGH is a feasible and safe option for aneuploidy screening that shows excellent outcomes when used in fresh cycles. This is the first report of cleavage stage PGS by CGH showing improved ongoing pregnancy rates.

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Section: Discussionsupporting

confidence: 88%

Preimplantation Genetic Screening (PGS) with Comparative Genomic Hybridization (CGH) following day 3 single cell blastomere biopsy markedly improves IVF outcomes while lowering multiple pregnancies and miscarriages

Keltz

Vega

Sirota

et al. 2013

J Assist Reprod Genet

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“…This is particularly true in light of the experiences with FISH-based aneuploidy screening which was suggested to be clinically beneficial based on case–control studies ( Gianaroli et al ., 1997 ; Munne et al ., 1999 ; Kahraman et al ., 2000 ; Munne et al ., 2003 ; Munne et al ., 2006 ), despite failing to show a meaningful benefit in all randomized controlled clinical trials (reviewed in Fritz, 2008 ). Although similar case–control and observational clinical studies using comprehensive methods of aneuploidy screening have been reported ( Wells et al ., 2009 ; Munne et al ., 2010 ; Rabinowitz et al ., 2010 ; Schoolcraft et al ., 2010a ,b), randomized controlled trials have been limited. Indeed, class I strength of evidence for a significant improvement in clinical pregnancy and embryo implantation rates (i.e.…”

Section: Introductionmentioning

confidence: 99%

Single-cell whole-genome amplification technique impacts the accuracy of SNP microarray-based genotyping and copy number analyses

Treff

Tao

et al. 2010

MHR: Basic Science of Reproductive Medicine

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Methods of comprehensive microarray-based aneuploidy screening in single cells are rapidly emerging. Whole-genome amplification (WGA) remains a critical component for these methods to be successful. A number of commercially available WGA kits have been independently utilized in previous single-cell microarray studies. However, direct comparison of their performance on single cells has not been conducted. The present study demonstrates that among previously published methods, a single-cell GenomePlex WGA protocol provides the best combination of speed and accuracy for single nucleotide polymorphism microarray-based copy number (CN) analysis when compared with a REPLI-g- or GenomiPhi-based protocol. Alternatively, for applications that do not have constraints on turnaround time and that are directed at accurate genotyping rather than CN assignments, a REPLI-g-based protocol may provide the best solution.

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“…Although largely clinically un-validated, comparative data with other platforms suggest better efficiency than both FISH and aCGH for aneuploidy screening (Johnson et al 2010b;Treff et al 2010a;Treff et al 2010b). Recently presented clinical data of SNP array based PGS on cleavage stage embryos suggests significant improvement of pregnancy rates following embryo transfer (Rabinowitz et al 2010).We anticipate that with further clinical validation, SNP genotyping will become the gold standard for PGS in the near future.…”

Section: Single Nucleotide Polymorphism (Snp) Arraysmentioning

confidence: 98%

The Role of Aneuploidy Screening in Human Preimplantation Embryos

Christian¹,

Darren²,

Alan³

2012

Aneuploidy in Health and Disease

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Effects of advanced maternal age are abrogated in 122 patients undergoing transfer of embryos with euploid microarray screening results at cleavage stage

Cited by 5 publications

References 0 publications

Preimplantation Genetic Screening (PGS) with Comparative Genomic Hybridization (CGH) following day 3 single cell blastomere biopsy markedly improves IVF outcomes while lowering multiple pregnancies and miscarriages

Preimplantation Genetic Screening (PGS) with Comparative Genomic Hybridization (CGH) following day 3 single cell blastomere biopsy markedly improves IVF outcomes while lowering multiple pregnancies and miscarriages

Single-cell whole-genome amplification technique impacts the accuracy of SNP microarray-based genotyping and copy number analyses

The Role of Aneuploidy Screening in Human Preimplantation Embryos

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