Effects of adrenaline in human colon adenocarcinoma HT-29 cells

Wong, Hai Ming; Ho, Judy W. C.; Koo, Marcel W.L.; Yu, Li; Wu, William Ka Kei; Lam, Emily Kai Yee; Tai, Emily Kin Ki; Ko, Jae-Ki; Shin, Vivian Y.; Chu, Kent Man; Cho, Chi Hin

doi:10.1016/j.lfs.2011.04.007

Cited by 58 publications

(48 citation statements)

References 25 publications

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“…These results are confirmed by another study that showed a 35% increase of HT29 cell proliferation induced by epinephrine treatment at the concentration of 10 µM (67). Other research on HT29, SW116 and LS174T colorectal carcinoma cell lines showed that the stress hormones epinephrine and norepinephrine at concentrations of 0.1-10 µM markedly increase the cell proliferation with no significant effect induced by corticosterone (53).…”

Section: Discussionsupporting

confidence: 62%

Catecholamines Increase in Vitro Proliferation of Murine B16F10 Melanoma Cells

Căruntu¹

2014

Acta Endo (Buc)

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Context.Melanoma is the most aggressive skin cancer, with significant morbidity and mortality, and one factor that may influence the course of disease is stress.Objective. Our aim was to evaluate the effect of corticosterone, norepinephrine, epinephrine on murine B16F10 melanoma cells in vitro proliferation.Methods. B16F10 melanoma cells were treated with different concentrations of tested hormones. The proliferative capacity of melanoma cells was quantified by MTS assay and the cell viability was quantified as membrane integrity evaluation measured by lactate dehydrogenase (LDH) release.Results. B16F10 cells treated with corticosterone showed no significant changes. In contrast, norepinephrine exposure stimulated the cell proliferation (P = 0.0003). Treatment with 1 µM norepinephrine induced the highest increase in cell proliferation (OD 492 = 0.27 ± 0.02) statistically significant to both control (OD 492 = 0.17 ± 0.01; p = 0.0003), 10 nM norepinephrine (OD 492 = 0.16 ± 0.00; p = 0.0004) and 100 nM norepinephrine (OD 492 = 0.19 ± 0.01; p = 0.002). Likewise, treatment with epinephrine increased cell proliferation (p = 0.0004). Exposure to 5 µm epinephrine induced a stimulation of cell proliferation (OD 492 = 0.28 ± 0.02) significantly higher compared to controls (OD 492 = 0.17 ± 0.01; p = 0.0004), 50 nM epinephrine (OD 492 = 0.17 ± 0.00; p = 0.001) and 500 nM epinephrine (OD 492 = 0.173 ± 0.00; p = 0.001). Conclusions.Our results may open new perspectives concerning the link between stress hormones and melanoma, emphasizing a direct stimulating in vitro effect induced by catecholamines on melanoma B16F10 cells proliferation.

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Section: Discussionsupporting

confidence: 62%

Catecholamines Increase in Vitro Proliferation of Murine B16F10 Melanoma Cells

Căruntu¹

2014

Acta Endo (Buc)

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“…Others have shown in vitr o that catecholamines stimulate migration and proliferation of human colon cancer cell lines. 19,20 On-going treatment of our patient with alpha- and beta-adrenergic blockers may have minimized the effects of these agents. Additionally, we were unable to measure acetylcholine levels in our patient’s serum.…”

Section: Discussionmentioning

confidence: 99%

Bedside to Bench: Role of Muscarinic Receptor Activation in Ultrarapid Growth of Colorectal Cancer in a Patient With Pheochromocytoma

Rosenvinge

Cheng

Drachenberg

et al. 2013

Mayo Clinic Proceedings

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An elderly man with long-standing, non-resectable pheochromocytoma rapidly developed rectal adenocarcinoma despite close endoscopic surveillance. We determined that the patient’s colorectal cancer over-expressed M3 subtype muscarinic receptors, whereas his pheochromocytoma expressed choline acetyltransferase, an enzyme required to produce acetylcholine, a muscarinic receptor agonist. These findings suggested that acetylcholine release from the pheochromocytoma stimulated rapid growth of the rectal neoplasm. As proof-of-principle, we demonstrate here that culture media conditioned by pheochromocytoma cells stimulates proliferation of a human colon cancer cell line, an effect attenuated by atropine, a muscarinic receptor inhibitor. Our observations provide both clinical and laboratory evidence that muscarinic receptor agonists promote the growth of colorectal neoplasia.

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“…17 Recent studies suggest these receptors are critical for the development of colorectal cancer. 18 The third drug is methyprylon, a sedative of the piperidinedione derivative family and a treatment for insomnia. Up-regulation of microRNA miR-155 inhibits γ-aminobutyric acid A receptor 1 (GABRA1, target of methyprylon) and promotes tumor growth.…”

Section: Resultsmentioning

confidence: 99%

Exploring a structural protein–drug interactome for new therapeutics in lung cancer

Peng

Wang

et al. 2014

Mol. BioSyst.

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The pharmacology of drugs is often defined by more than one protein target. This property can be exploited to use approved drugs to uncover new targets and signaling pathways in cancer. Towards enabling a rational approach to uncover new targets, we expand a structural protein-ligand interactome (http://www.biodrugscreen.org) by scoring the interaction among 1,000 FDA-approved drugs docked to 2,500 pockets on protein structures of the human genome. This afforded a drug-target network whose properties compared favorably with previous networks constructed with experimental data. Among drugs with highest degree and betweenness two are cancer drugs and one is currently used for treatment of lung cancer. Comparison of predicted cancer and non-cancer targets reveals that the most cancer-specific compounds were also the most selective compounds. Analysis of compound flexibility, hydrophobicity, and size showed that the most selective compounds were low molecular weight fragment-like heterocycles. We use a previously-developed screening approach using the cancer drug erlotinib as a template to screen other approved drugs that mimic its properties. Among the top 12 ranking candidates, four are cancer drugs, two of them kinase inhibitors (like erlotinib). Cellular studies using non-small cell lung cancer (NSCLC) cells revealed that several drugs inhibited lung cancer cell proliferation. We mined patient records at the Regenstrief Medical Record System to explore possible association of exposure to three of these drugs with occurrence of lung cancer. Preliminary in vivo studies using non-small cell lung cancer (NCLSC) xenograft model showed that losartan- and astemizole-treated mice had tumors that weighed 50 (p < 0.01) and 15 (p < 0.01) percent less than vehicle. These results set the stage for further exploration of these drugs and to uncover new drugs for lung cancer.

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Effects of adrenaline in human colon adenocarcinoma HT-29 cells

Cited by 58 publications

References 25 publications

Catecholamines Increase in Vitro Proliferation of Murine B16F10 Melanoma Cells

Catecholamines Increase in Vitro Proliferation of Murine B16F10 Melanoma Cells

Bedside to Bench: Role of Muscarinic Receptor Activation in Ultrarapid Growth of Colorectal Cancer in a Patient With Pheochromocytoma

Exploring a structural protein–drug interactome for new therapeutics in lung cancer

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