Effects of adolescent alcohol exposure via oral gavage on adult alcohol drinking and co-use of alcohol and nicotine in Sprague Dawley rats

Chandler, Cassie M.; Hamid, Usman Z.; Maggio, Sarah E.; Peng, Hui; Pauly, James R.; Beckmann, Joshua S.; Nixon, Kimberly; Bardo, Michael T.

doi:10.1016/j.drugalcdep.2022.109298

Cited by 5 publications

(8 citation statements)

References 57 publications

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“…Males and females displayed widespread differences in resource acquisition. Water consumption was evaluated by controlling for body weight 29 – 31 . A two-way between-subject ANOVA with sex as the between-subject factor and delay (0, 6, 12, 18, and 24 s) as the within-subject factor was conducted to determine if sex moderated water consumption (µL/min/kg).…”

Section: Resultsmentioning

confidence: 99%

Reward maximization assessed using a sequential patch depletion task in a large sample of heterogeneous stock rats

Gancarz

Mitchell

George

et al. 2023

Sci Rep

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Choice behavior requires animals to evaluate both short- and long-term advantages and disadvantages of all potential alternatives. Impulsive choice is traditionally measured in laboratory tasks by utilizing delay discounting (DD), a paradigm that offers a choice between a smaller immediate reward, or a larger more delayed reward. This study tested a large sample of Heterogeneous Stock (HS) male (n = 896) and female (n = 898) rats, part of a larger genetic study, to investigate whether measures of reward maximization overlapped with traditional models of delay discounting via the patch depletion model using a Sequential Patch Depletion procedure. In this task, rats were offered a concurrent choice between two water “patches” and could elect to “stay” in the current patch or “leave” for an alternative patch. Staying in the current patch resulted in decreasing subsequent reward magnitudes, whereas the choice to leave a patch was followed by a delay and a resetting to the maximum reward magnitude. Based on the delay in a given session, different visit durations were necessary to obtain the maximum number of rewards. Visit duration may be analogous to an indifference point in traditional DD tasks. Males and females did not significantly differ on traditional measures of DD (e.g. delay gradient; AUC). When examining measures of patch utilization, females made fewer patch changes at all delays and spent more time in the patch before leaving for the alternative patch compared to males. Consistent with this, there was some evidence that females deviated from reward maximization more than males. However, when controlling for body weight, females had a higher normalized rate of reinforcement than males. Measures of reward maximization were only weakly associated with traditional DD measures and may represent distinctive underlying processes. Taken together, females performance differed from males with regard to reward maximization that were not observed utilizing traditional measures of DD, suggesting that the patch depletion model was more sensitive to modest sex differences when compared to traditional DD measures in a large sample of HS rats.

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Section: Resultsmentioning

confidence: 99%

Reward maximization assessed using a sequential patch depletion task in a large sample of heterogeneous stock rats

Gancarz

Mitchell

George

et al. 2023

Sci Rep

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“…Fundamental to alcohol problems in adulthood is increased consumption, the effect we hoped to model here. We previously observed no escalation in alcohol drinking after bingelike ethanol in both 4-day (unpublished observations) and 2-day exposure via intragastric gavage [43] as well as with voluntary consumption via two-bottle choice [42]. Therefore, we aimed to replicate a model of adolescent alcohol exposure that was documented to escalate adult drinking: a 2 g/kg dose was administered i.p.…”

Section: Discussionmentioning

confidence: 99%

“…Adolescent male Sprague Dawley rats (n = 64) were purchased from Charles River Laboratories (Raleigh, NC, USA) and arrived at our facility at postnatal day (PND) 21. Male rats were utilized because our lab has previously shown that under a forced consumption paradigm, adolescent alcohol exposure impacted adult drinking in male rats only [43] and past experiments that we were attempting to replicate were conducted in males only [36]. All rats were acclimated to polycarbonate cages in an AAALAC-accredited vivarium at The University of Texas at Austin with a 12:12 h light/dark cycle and ad libitum access to rat chow and water for 7 days before experiments began.…”

Section: Animals and Housing Conditionsmentioning

confidence: 99%

“…Exposure to alcohol during adolescence also escalates adult drinking in some animal models [36][37][38][39][40][41] but not others, e.g., [42][43][44][45][46]. For example, in rats, escalation in drinking following adolescent exposure has been modeled with adolescent intermittent ethanol exposure (AIE) followed by testing two-bottle free-choice (2BC) alcohol drinking in adulthood [36,37,39,47].…”

Section: Introductionmentioning

confidence: 99%

“…For example, in rats, escalation in drinking following adolescent exposure has been modeled with adolescent intermittent ethanol exposure (AIE) followed by testing two-bottle free-choice (2BC) alcohol drinking in adulthood [36,37,39,47]. However, both forced exposure, such as binge models via intragastric gavage or as sole source of liquids, and some voluntary consumption models, such as 2BC, have failed to produce an escalation in adult alcohol drinking [42][43][44][45][46]. One commonality among the models that successfully escalated adult drinking is single housing of the animals.…”

Section: Introductionmentioning

confidence: 99%

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Adolescent Intermittent Ethanol Drives Modest Neuroinflammation but Does Not Escalate Drinking in Male Rats

Wooden,

Peacoe,

Anasooya Shaji

et al. 2023

Cells

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During adolescence, the brain is highly susceptible to alcohol-induced damage and subsequent neuroimmune responses, effects which may enhance development of an alcohol use disorder (AUD). Neuroimmune reactions are implicated in adolescent alcohol exposure escalating adulthood drinking. Therefore, we investigated whether intermittent alcohol exposure in male, adolescent rats (AIE) escalated adult drinking via two-bottle choice (2BC). We also examined the influence of housing environment across three groups: standard (group-housed with enrichment during 2BC), impoverished (group-housed without enrichment during 2BC), or isolation (single-housed without bedding or enrichment throughout). In the standard group immediately after AIE/saline and after 2BC, we also examined the expression of microglial marker, Iba1, reactive astrocyte marker, vimentin, and neuronal cell death dye, FluoroJade B (FJB). We did not observe an escalation of adulthood drinking following AIE, regardless of housing condition. Further, only a modest neuroimmune response occurred after AIE in the standard group: no significant microglial reactivity or neuronal cell death was apparent using this model, although some astrocyte reactivity was detected in adolescence following AIE that resolved by adulthood. These data suggest that the lack of neuroimmune response in adolescence in this model may underlie the lack of escalation of alcohol drinking, which could not be modified through isolation stress.

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Effects of voluntary adolescent intermittent alcohol exposure and social isolation on adult alcohol intake in male rats

Chandler¹,

Shaykin²,

Peng³

et al. 2022

Alcohol

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Effects of adolescent alcohol exposure via oral gavage on adult alcohol drinking and co-use of alcohol and nicotine in Sprague Dawley rats

Cited by 5 publications

References 57 publications

Reward maximization assessed using a sequential patch depletion task in a large sample of heterogeneous stock rats

Reward maximization assessed using a sequential patch depletion task in a large sample of heterogeneous stock rats

Adolescent Intermittent Ethanol Drives Modest Neuroinflammation but Does Not Escalate Drinking in Male Rats

Effects of voluntary adolescent intermittent alcohol exposure and social isolation on adult alcohol intake in male rats

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