Effects of administration of adipose-derived stromal vascular fraction and platelet-rich plasma to dogs with osteoarthritis of the hip joints

Abstract

OBJECTIVE To evaluate effects of simultaneous intra-articular and IV injection of autologous adipose-derived stromal vascular fraction (SVF) and platelet-rich plasma (PRP) to dogs with osteoarthritis of the hip joints. ANIMALS 22 client-owned dogs (12 placebo-treated [control] dogs and 10 treated dogs). PROCEDURES Dogs with osteoarthritis of the hip joints that caused signs of lameness or discomfort were characterized on the basis of results of orthopedic examination, goniometry, lameness score, the Canine Brief Pain Inventory (CBPI), a visual analogue scale, and results obtained by use of a pressure-sensing walkway at week 0 (baseline). Dogs received a simultaneous intraarticular and IV injection of SVF and PRP or a placebo. Dogs were examined again 4, 8, 12, and 24 weeks after injection. RESULTS CBPI scores were significantly lower for the treatment group at week 24, compared with scores for the control group. Mean visual analogue scale score for the treatment group was significantly higher at week 0 than at weeks 4, 8, or 24. Dogs with baseline peak vertical force (PVF) in the lowest 25th percentile were compared, and the treatment group had a significantly higher PVF than did the control group. After the SVF-PRP injection, fewer dogs in the treated group than in the control group had lameness confirmed during examination. CONCLUSIONS AND CLINICAL RELEVANCE For dogs with osteoarthritis of the hip joints treated with SVF and PRP, improvements in CBPI and PVF were evident at some time points, compared with results for the control group.

Comparison of Platelet-Rich Plasma, Stromal Vascular Fraction (SVF), or SVF with an Injectable PLGA Nanofiber Scaffold for the Treatment of Osteochondral Injury in Dogs

“…The surgical incision was closed routinely, and 20 g of falciform adipose tissue was processed using a commercially available kit for patient side SVF processing (ActiStem, MediVet Biologics, Nicholasville, KY) as has been described previously. 10 Briefly, preparation of the SVF involves collagenase digestion, centrifugation, and isolation of the SVF pellet. 10 The previously prepared plasma is then activated with a proprietary calcium chloride solution (Solution G, MediVet Biologics) and the SVF pellet is suspended in 2 mL of the activated plasma releasate.…”

“…[1][2][3] Stromal vascular fraction (SVF) is a point-of-care therapy that is typically prepared by the harvest of autologous adipose tissue followed by mechanical and chemical digestion and subsequent centrifugation to isolate the cellular SVF pellet from the remaining lipid and extracellular components. 10 There is no culture expansion or homogenization of the cells in the SVF so usually fewer than 5% of the cells in the SVF meet even lenient definitions of being a stem cell. [11][12][13] This is in contrast to cultured stem cell therapy in which cells are harvested from either fat or bone marrow and cultureexpanded in a laboratory over a period of weeks, a process that homogenizes the cell population while also dramatically increasing the number of available cells.…”

“…21,24 In dogs, there are two recent prospective, randomized, controlled trials with objective functional outcome measures; these studies did not provide consistent evidence of benefit based upon those objective kinetic metrics. 10,25 Finally, we are aware of only one study in a large animal model (equine) that has evaluated cartilage biochemistry and histology after SVF treatment, which reported no histologic, histochemical, or biochemical improvements. 26 As a result, there remains uncertainty as to whether the use of SVF is truly beneficial in terms of symptom relief, functional improvements, diseasemodifying effects or cartilage regenerative capacity.…”

The Use of Allogenic Stromal Vascular Fraction (SVF) Cells in Degenerative Joint Disease of the Spine in Dogs

“…Clinical examinations were conducted 4, 8, 12, and 24 weeks after the injection. The researchers have reported a significant reduction in lameness and pain during the orthopaedic examination, and the therapeutic effect persisted for several months (42).…”

A report on the use of a single intra-articular administration of autologous platelet therapy in a naturally occurring canine osteoarthritis model - a preliminary study

“…The best ones developed for dogs, and that have been reported to have criterion validity, are the Canine Brief Pain Inventory (CBPI) and the Liverpool Osteoarthritis in Dogs (LOAD) [9,[14][15][16]. The CBPI is divided in two sections, a pain severity score (PSS), that assesses the magnitude of the animal pain, and a pain interference score (PIS), that assesses the degree in which pain affects daily activities [17]. The Canine Orthopaedic Index (COI) was developed to assess four domains in dogs with OA: stiffness, gait, function and quality of life (QOL).…”