Effects of administering testosterone undecanoate in rats subjected to physical exercise: effects on the estrous cycle, motor behavior and morphology of the liver and kidney

Abstract

The aim of the work was evaluate the effects of testosterone undecanoate (TU) treatment combined with moderate physical training on: the estrous cycle, body weight (BW), motor behavior (MB), and the morphohistology of the reproductive system, the liver and kidney in rats. Female Wistar rats (180 g -250 g) were divided as follows: sedentary + TU (S + TU), trained + TU (T + TU), sedentary + vehicle (S + V), trained + vehicle (T + V). The rats swam 50 min/Day, strapped with a 5% BW load, for 4 weeks. During this training, (BW) was monitored daily as well as the estrous cycle (EC) by vaginal smear. The TU (15 mg/kg s.c) was administered 3 times/week for 4 weeks. At the end of the study, data on MB, BW and morphohistopathological changes in viscera were compiled. The (T + TU) group had on average, a higher (BW) in the fourth week compared to the first week, and (BW) higher than (S + V) and (S + TU) groups. We noted an interruption in the EC and a decrease in weight of ovaries in animals treated with TU. In addition, there was an increase in the relative weight of the heart in groups (T + V) and (T+ TU), and kidneys in group (T + TU). Histopathological analysis showed periportal congestion and isolated foci of hepatic necrosis in rats with TU. Thus, TU combined with training abolished the EC, promoted ovarian atrophy, liver necrosis, cardiac hypertrophy and a decrease in motor activity.Uniterms: Androgenic anabolic steroids/experimental study. Endurance training. Testosterone undecanoate.O objetivo do trabalho foi avaliar o efeito do tratamento com undecanoato de testosterona (UT) combinado ao treinamento físico moderado sobre ciclo estral, peso corporal, estruturas do sistema reprodutor, comportamento motor e morfologia hepática e renal em ratas. Ratas Wistar (180 a 250 g) foram divididas em: sedentárias + UT (S+UT), treinadas + UT (T+UT), sedentárias + veículo (S+V), treinadas + veículo (T+V). As ratas nadaram 50 min/dia com sobrecarga de ~5% do peso corporal por 4 semanas. Durante o período de treinamento foi realizado acompanhamento diário do peso corporal (PC) e do ciclo estral (CE) pelo esfregaço vaginal. O UT (15 mg/kg s.c.) foi administrado 3x/semana durante 4 semanas. Ao final foram avaliados comportamento motor, pesos e alterações histopatológicas de alguns órgãos. O grupo T+UT apresentou PC maior na quarta semana do que na primeira, com pesos corporais maiores que os grupos S+V e S+UT. Houve interrupção no CE e redução do peso dos ovários nos animais tratados com UT. Houve aumento do peso relativo do coração, nos grupos T+V e T+UT, e do peso relativo dos rins, no grupo T+UT. A análise histopatológica revelou congestão periportal e focos isolados de necrose hepática nas ratas com UT. O UT combinado com treinamento produziu supressão do ciclo estral, atrofia ovariana, necrose hepática, hipertrofia cardíaca e redução da atividade motora.Unitermos: Esteróides androgênicos anabólicos/estudo experimental. Exercício físico. Undecanoato de testosterona.

Effect of Nandrolone Decanoate (Anabolic Steroid) on the Liver and Kidney of Male Albino Rats and the Role of Antioxidant (Antox-Silymarin) as Adjuvant Therapy

“…This was in agreement with another study, who studied the effects of testosterone undecanoate (TU) treatment reported degeneration of the kidney were found in 25% of the animals treated with TU [22,23].…”

Effects of different doses of nandrolone decanoate on estrous cycle and ovarian tissue of rats after treatment and recovery periods

“…In the groups treated with highest doses, the ovarian weight remained unchanged. It is well documented that the AAS promotes reduction in reproductive organ weights (Camargo et al 2009a(Camargo et al ,b, 2014Bento-Silva et al 2010;Karbalay-Doust & Noorafshan 2012). The suppression of estrous cyclicity caused by AAS treatment was previously reported in various studies (Gerez et al 2005;Mobini Far et al 2007;Bento-Silva et al 2010;Camargo et al 2011Camargo et al , 2014Chuffa et al 2011b) and severely affects the sexual cycle of women (Maravelias et al 2005).…”

“…The first injection was administered at the estrus phase of the cycle in all animals. The CD group was used in this study based on previous reports that AAS treatment promotes persistent diestrus (Camargo et al 2009a,b;Bento-Silva et al 2010;Chuffa et al 2011b). In this regard, the evaluation of ovarian tissue was carried out in a specific stage of the estrous cycle that androgenized females persisted or maintained for a long time during the experimental period.…”

Dose‐Dependent Effects and Reversibility of the Injuries Caused by Nandrolone Decanoate in Uterine Tissue and Fertility of Rats

“…The estrous acyclicity promoted by the treatment of ND is widely reported (Clark et al., ; Gerez et al., ; Mobini Far et al., ; Camargo et al., ,; Bento‐Silva et al., ; Chuffa et al., ), and this effect is related to a disruption of the neuroendocrine functions, in which high levels of exogenous testosterone alter the release of the Follicle stimulating hormone (FSH), Luteinizing hormone (LH), estrogen, and progesterone (Strauss et al., ; Gao and Short, ; Bronson et al., ; Blasberg et al., ). The persistence in diestrous found in ND‐treated animals and after 30‐day recovery indicate that nandrolone is slowly released from the injection site into the blood circulation with a half‐life of 6 to 8 days (Van der Vies, ), since it is a fat‐soluble substance that produces sodium retention by the kidneys before being excreted.…”