Effects of adipose tissue-derived stromal vascular fraction on osteochondral defects treated by hyaluronic acid-based scaffold: An experimental study

Abstract

Objectives: This study aims to evaluate the effect of adipose-derived stromal vascular fraction (SVF) on osteochondral defects treated by hyaluronic acid (HA)-based scaffold in a rabbit model. Materials and methods: Eighteen white New Zealand rabbits were randomly grouped into the experimental group (n=9) and control group (n=9). In all groups, osteochondral defects were induced on the weight-bearing surfaces of the right femoral medial condyles, and a HA-based scaffold was applied to the defect area with microfractures (MFs). In this study, 1 mL of adipose-derived SVF was injected into the knee joints of the rabbits in the experimental group. For histological and macroscopic evaluation, four rabbits were randomly selected from each group at Week 4, and the remaining rabbits were sacrificed at the end of Week 8. Macroscopic assessments of all samples were performed based on the Brittberg scoring system, and microscopic evaluations were performed based on the O’Driscoll scores. Results: Samples were taken at Weeks 4 and 8. At Week 4, the O’Driscoll scores were significantly higher in the control group than the experimental group (p=0.038), while there was no significant difference in the Brittberg scores between the two groups (p=0.108). At Week 8, the O’Driscoll score and Brittberg scores were statistically higher in the experimental group than in the control group (p=0.008 and p=0.007, respectively). According to the microscopic evaluation, at the end of Week 8, the cartilage thickness was greater in the experimental group, and nearly all of the defect area was filled with hyaline cartilage. Conclusion: Application of adipose-derived SVF with MF-HA-based scaffold was better than MF-HA-based scaffold treatment in improving osteochondral regeneration. Therefore, it can be used in combination with microfracture and scaffold to accelerate cartilage regeneration, particularly in the treatment of secondary osteoarthritis.

Investigation of the efficacy of epidermal growth factor, boric acid and their combination in cartilage injury in rats: An experimental study

“…The cytotoxic effect of the combinations of EGF dilutions (1,5,10,25, 50, 100, 200 ng/mL) with BA (100, 300, 500 µg/mL) was observed only in the 72-h application period and in Saos-2. The cytotoxic effect of BA was reduced when combined with EGF.…”

“…The L929, Saos-2, and hAD-MSCs were seeded at 7¥10 3 cells/well in 96-well plates and incubated at 37°C and 5% CO 2 for 24 h. One day later, BA dilutions (10,20,50,100,200, 300, 500, 1,000, 3,000, 10,000, and 30,000 µg/mL), EGF dilutions (1, 5, 10, 25, 50, 100, and 200 ng/mL) were prepared in the DMEM and each dilution was added into the six wells of the 96-well plate. On the other hand, to evaluate synergistic, agonistic, antagonistic, or/and additive effects, each EGF dilution (1,5,10,25,50, 100, and 200 ng/mL) was combined with: (i) 100 µg/mL BA; (ii) 300 µg/mL BA; and (iii) 500 µg/mL BA) and added six wells of the 96-well plates. Then, 96-well plates were incubated at 37°C and 5% CO 2 for 24, 48, and 72 h. After each incubation period, DMEM containing 10% of MTT [3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide] solution (5 mg/mL in DPBS, Sigma-Aldrich #M5655) was added and the cells were incubated for 4 h. Then, 100 µL of dimethyl sulfoxide (DMSO) was added to each well of the plate on a shaker for 2 h at room temperature to dissolve the purple formazan crystals formed due to the metabolic activity of living cells.…”

“…Due to its avascular nature and restricted metabolic activity of mature chondrocytes, cartilage tissue is unable to self-repair after injury. [ 1 ] In the absence of treatment, cartilage damage may initiate degenerative processes in the adjacent cartilage tissue and subchondral bone, which may eventually result in OA. [ 2 ] Treatments for chondral damage include arthroscopic debridement,[ 3 ] artificial joint replacement,[ 4 ] autologous chondrocyte transplantation,[ 5 ] chondral and osteochondral grafts,[ 6 ] and bone marrow stimulation.…”

Mid-term results of autologous matrix-induced chondrogenesis surgery with or without scaffolds for arthroscopic treatment of deep talus osteochondral lesions: A comparative study

“…[ 1 ] Although microfracture, abrasion chondroplasty, curettage, drilling, mosaicplasty, and cellular or noncellular scaffolds have been used in the treatment of OLT, the most optimal treatment method is often determined based on the lesion size and depth. [ 2 , 3 ]…”

“…[1] Although microfracture, abrasion chondroplasty, curettage, drilling, mosaicplasty, and cellular or noncellular scaffolds have been used in the treatment of OLT, the most optimal treatment method is often determined based on the lesion size and depth. [2,3] Osteochondral lesion of the talus was first classified by Berndt and Harty [4] in 1959. The Bristol classification has been more frequently used in recent years toObjectives: This study aims to investigate the effectiveness of arthroscopic autologous matrix-induced chondrogenesis (AMIC) procedure with or without polyglycolic acid-hyaluronic acid (PGA-HA)-based cell-free scaffold (CFS) in Bristol Stage 4 and Stage 5 osteochondral lesion of the talus (OLT) ranging between 1.5 and 3 cm 2 .Patients and methods: Between March 2018 and March 2021, a total of 47 patients with OLTs (29 males, 18 females; mean age: 22.8±2.3 years; range, 18 to 65 years) were retrospectively analyzed.…”