Effects of Adhesive or Non-adhesive Protein-immobilized Bioactive（Lactic Acid-ε-caprolactone）Films on Mouse Osteoblast-like Cells

Abstract

This study investigated properties of the fabricated polylactideco-ε-caprolactone (PLCL50) with polylactic acid (PLA) and poly-ε-caprolactone (PCL) in an equal ratio and the fibronectin (FN) and albumin (Alb) , immobilized PLCL films, to apply them for barrier membranes for reconstructive bone augmentation.FN is a cell adhesion protein, and Alb is a cell adhesioninhibiting protein. The contact angle of PLCL50 -COOH, FN -PLCL50, and Alb -PLCL50 surfaces with double distilled water significantly decreased than the PLCL50 surface in the evaluation using film surface wettability analysis. The presence of fibronectin and albumin on PLCL50 films was demonstrated by Xray photoelectron spectroscopy analysis (XPS) . The number of cells attached to FN -PLCL50 films was more significant than those attached to the Alb -PLCL50 and PLCL50 films after 90 min and 3 days of culture. On the other hand, immobilizing albumin on the PLCL50 surface resulted in a significant decrease in attached cells after 90 min and 3 days. The quantification of mineralization of osteoblast MC3T3 -E1 cells on FN -PLCL50 films was significantly higher than that of Alb -PLCL50 and PLCL50 films after 7, 14 and 21 days in the results of alizarin red staining and mineralization assay. The morphological differences in the attached MC3T3 -E1 cells on FN -PLCL50, Alb -PLCL50 and PLCL50 were imaged by scanning electron microscopy (SEM) . After 90 min, 3 and 7 days of culture, the attached MC3T3 -E1 cells on the FN -PLCL50 films were more spread than those on the Alb -PLCL50 and PLCL50 films.These results suggested that fibronectin and albumin effectively performed biological activities on PLCL50 films of osteoblastlike cells.