Effects of adenovirus‐expressing IL‐10 in alleviating airway inflammation in asthma

Abstract

Our data showed that the intratracheal transfer of the IL-10 gene could affect the recruitment of inflammatory cells during the challenge phase in a way that would result in the inhibition of airway inflammation. These findings suggest that the development of an immunoregulatory strategy based on IL-10 might shed light on more effective treatment.

Aerobic Exercise Decreases Chronic Allergic Lung Inflammation and Airway Remodeling in Mice

“…Activated skeletal muscle releases increased amounts of IL-10 during aerobic training sessions, underlying part of the antiinflammatory effects of aerobic exercise in cardiovascular disease and type 2 diabetes (42). IL-10 also presents antiinflammatory effects in experimental models of chronic allergic lung inflammation (43,44). Our results demonstrated that in sensitized animals, aerobic exercise training increased the expression of IL-10 by inflammatory cells, representing a possible mechanism of exercise-induced decrease in allergic inflammation.…”

The pros and cons of immunomodulatory IL-10 gene therapy with recombinant AAV in a Cftr−/−-dependent allergy mouse model

“…The systemic circulation of IL-10 would also lower IgE levels, which was not achieved in previous studies using local delivery of AAV1-IL-10. 58 Although our results indicate that IL-10 acted to inhibit pulmonary eosiniophila and inflammation and was successful at lowering both IgE levels and proinflammatory cytokine production, they also uncovered serious side effects associated with sustained IL-10 serum levels. Serum IL-10 levels correlated with thrombocytopenia, splenomegaly and liver inflammation.…”

“…[54][55][56][57] Intratracheal delivery of adenoviral vectors expressing IL-10 have been previously shown to decrease lung inflammation but not IgE levels in an ovalbumin asthma mouse model. 58 However, to this date clinical studies with IL-10 remain difficult due to its short half-life (1.1-2.6 h) in serum and the requirement for subcutaneous or intravenous routes of administration. 59 The studies described here were performed in a previously described Cftr À/À mouse model that is characterized by a peculiar hyper-IgE phenotype, in response to A. fumigatus crude protein extract (Af-cpe).…”

The Escherichia coli heat-labile enterotoxin B subunit protects from allergic airway disease development by inducing CD4+ regulatory T cells

“…Production of these cytokines by either cell type in the lung could have a role in modifying the environment to suppress lung pathology. 34,35 An increased concentration of IL-10 in the BAL was linked to the suppression of asthma following adoptive transfer of OVA-specific CD4 þ CD25 þ Treg, 29 and our studies showed a slight increase in IL-10 in the BAL of treated animals. IL-10 can suppress the secretion of other cytokines and control cellular activation, consistent with modulating pathology.…”