Effects of Adenosine Infusion on the Pig Heart During Normothermic Ischemia and Reperfusion

Abstract

Possible enhancement of myocardial protection during ischemia and reperfusion by administration of adenosine was evaluated in a pig heart model. Adenosine (100 micrograms/kg/min) was infused into the aortic root during ischemia in group AI (n = 5) and into the right atrium during reperfusion in group AR (n = 6). Group C (n = 6) served as controls. During cardiopulmonary bypass the hearts were subjected to 30 min of normothermic ischemia and 15 min of reperfusion before weaning. In group AI the stroke work index 30 and 90 min after ischemia and the mean arterial pressure 30 min after ischemia were significantly higher than in group C. These parameters did not differ significantly between groups AR and C. All groups showed decrease in myocardial adenosine triphosphate (ATP) and adenylate charge potential (ACP) during ischemia and partial (ATP) or complete (ACP) restoration after ischemia. Adenosine infusion into the aortic root during ischemia (adenosine cardioplegia) thus resulted in improved postischemic heart function, although biochemical correlates in ATP and ACP were not apparent.

Ro 44-9883, a New Non-Peptidic GPIIb-GPIIIa Antagonist Prevents Platelet Loss in a Guinea Pig Model of Extracorporeal Circulation

“…As a consequence, the platelet count was dramatically altered in our control group. Experimental ECC is usually performed either using in-vivo models with larger species, such as dogs (23)(24)(25), pigs (26)(27)(28), sheeps (29 , 30), or simply in-vitro models using anticoagulated human blood (10,15,19). When smaller species such as rabbits and rats were studied for ECC, the latter were mainly used for studying the thrombus formation in extracorporeal shunts (31), or for performing a locally controlled perfusion (32)(33)(34).…”