Effects of Adenosine and ATP on the Membrane Potential and Synaptic Transmission in Neurons of the Rat Locus Coeruleus

Kuwahata, Takashi

doi:10.2739/kurumemedj.51.109

Cited by 5 publications

(5 citation statements)

References 78 publications

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“…Hence the pressor response we observed does not appear to be elicited by adenosine. While recent studies have indicated a role for adenosine as well as ATP in synaptic transmission within the LC (Kuwahata, 2004), other similar studies concluded that adenosine was apparently released during hypoxia within the LC. Furthermore, while exogenous adenosine inhibited the spontaneous firing of LC neurones in a slice preparation, antagonism of adenosine receptors had no impact on spontaneous firing but did antagonise the effects of applied adenosine (Nieber et al ., 1995).…”

Section: Discussionmentioning

confidence: 98%

Purinergic modulation of cardiovascular function in the rat locus coeruleus

Yao

Lawrence

2005

British J Pharmacology

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1 The purpose of the present study was to determine whether purines exerted a physiological role in central cardiovascular modulation at the level of the locus coeruleus (LC). 2 In pentobarbitone-anaesthetised Wistar-Kyoto rats, unilateral microinjection of ATP or a,bmethyleneATP into the LC elicited dose-related decreases in blood pressure and heart rate. Unilateral microinjection of the P2 purinoceptor antagonists suramin and PPADS, caused pressor and tachycardic responses. Administration of the selective P2X 1 receptor antagonist NF-279 had no effect. While both ATP and L-glutamate (L-GLU) resulted in depressor responses after intra-LC microinjection, following intra-LC microinjection of P2 purinoceptor antagonists into the LC, the effects of subsequent administration of either ATP or L-GLU were functionally reversed, such that a pressor response ensued. 3 Microinjection of noradrenaline into the LC caused an increase in blood pressure and heart rate; however, the a 2 -adrenoceptor antagonist idazoxan had no cardiovascular effects, but did prevent the pressor response to PPADS or suramin. In addition, coinjection of idazoxan with either suramin or PPADS abolished the ATP and L-GLU mediated pressor responses observed following either suramin or PPADS administration. 4 The present data suggest that firstly, purines are capable of acting within the LC to ultimately modulate the cardiovascular system and secondly, that there is apparently a functional interaction between tonically active purinergic and noradrenergic systems within the LC of the rat.

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Section: Discussionmentioning

confidence: 98%

Purinergic modulation of cardiovascular function in the rat locus coeruleus

Yao

Lawrence

2005

British J Pharmacology

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“…On the other hand, Kuwahata (2004) demonstrated that ATP produced a hyperpolarizing response or a transient hyperpolarizing response that preceded the depolarization in LC neurons. However, AMP-PNP (adenosine 5 ′ -(β,γ-imido) triphosphate, which is more resistant to dephosphorylation than ATP, only depolarizes LC neurons.…”

Section: Neurochemical Modulation Of the Lcmentioning

confidence: 98%

“…However, AMP-PNP (adenosine 5 ′ -(β,γ-imido) triphosphate, which is more resistant to dephosphorylation than ATP, only depolarizes LC neurons. According to Kuwahata (2004) adenosine is the likely source of the ATP-induced hyperpolarizing response in LC neurons.…”

Section: Neurochemical Modulation Of the Lcmentioning

confidence: 99%

Neurochemical and electrical modulation of the locus coeruleus: contribution to CO2drive to breathe

et al. 2014

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The locus coeruleus (LC) is a dorsal pontine region, situated bilaterally on the floor of the fourth ventricle. It is considered to be the major source of noradrenergic innervation in the brain. These neurons are highly sensitive to CO2/pH, and chemical lesions of LC neurons largely attenuate the hypercapnic ventilatory response in unanesthetized adult rats. Developmental dysfunctions in these neurons are linked to pathological conditions such as Rett and sudden infant death syndromes, which can impair the control of the cardio-respiratory system. LC is densely innervated by fibers that contain glutamate, serotonin, and adenosine triphosphate, and these neurotransmitters strongly affect LC activity, including central chemoreflexes. Aside from neurochemical modulation, LC neurons are also strongly electrically coupled, specifically through gap junctions, which play a role in the CO2 ventilatory response. This article reviews the available data on the role of chemical and electrical neuromodulation of the LC in the control of ventilation.

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“…A healthy metabolism also plays a significant role in other higher order processes through its production of adenosine triphosphate (ATP), which, for example, allows the generation of a membrane potential. The membrane potential is itself crucial for a variety of functions, including maintaining the cell's structural integrity and the firing mechanism of neurons (Macknight, 1988;Kuwahata, 2004).…”

Section: Introductionmentioning

confidence: 99%

Modeling Cell Energy Metabolism as Weighted Networks of Non-autonomous Oscillators

Adams

Stefanovska

2021

Front. Physiol.

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Networks of oscillating processes are a common occurrence in living systems. This is as true as anywhere in the energy metabolism of individual cells. Exchanges of molecules and common regulation operate throughout the metabolic processes of glycolysis and oxidative phosphorylation, making the consideration of each of these as a network a natural step. Oscillations are similarly ubiquitous within these processes, and the frequencies of these oscillations are never truly constant. These features make this system an ideal example with which to discuss an alternative approach to modeling living systems, which focuses on their thermodynamically open, oscillating, non-linear and non-autonomous nature. We implement this approach in developing a model of non-autonomous Kuramoto oscillators in two all-to-all weighted networks coupled to one another, and themselves driven by non-autonomous oscillators. Each component represents a metabolic process, the networks acting as the glycolytic and oxidative phosphorylative processes, and the drivers as glucose and oxygen supply. We analyse the effect of these features on the synchronization dynamics within the model, and present a comparison between this model, experimental data on the glycolysis of HeLa cells, and a comparatively mainstream model of this experiment. In the former, we find that the introduction of oscillator networks significantly increases the proportion of the model's parameter space that features some form of synchronization, indicating a greater ability of the processes to resist external perturbations, a crucial behavior in biological settings. For the latter, we analyse the oscillations of the experiment, finding a characteristic frequency of 0.01–0.02 Hz. We further demonstrate that an output of the model comparable to the measurements of the experiment oscillates in a manner similar to the measured data, achieving this with fewer parameters and greater flexibility than the comparable model.

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Effects of Adenosine and ATP on the Membrane Potential and Synaptic Transmission in Neurons of the Rat Locus Coeruleus

Cited by 5 publications

References 78 publications

Purinergic modulation of cardiovascular function in the rat locus coeruleus

Purinergic modulation of cardiovascular function in the rat locus coeruleus

Neurochemical and electrical modulation of the locus coeruleus: contribution to CO2drive to breathe

Modeling Cell Energy Metabolism as Weighted Networks of Non-autonomous Oscillators

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