Effects of Adaptation of Infectious Bronchitis Virus Arkansas Attenuated Vaccine to Embryonic Kidney Cells

Ghetas, A. M.; Thaxton, G. E.; Breedlove, C.; Santen, Vicky L. van; Toro, Haroldo

doi:10.1637/10947-093014-reg

Cited by 18 publications

(22 citation statements)

References 38 publications

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“…Adaptation of an embryo-attenuated IBV ArkDPI-derived vaccine to CEK cell culture shifted the virus population towards homogeneity in S and NSP genes, and the changes achieved in the S1 gene in CEK-adapted virus were maintained after one backpassage in embryonating chicken eggs or chickens (6). Results of the present vaccination/challenge study indicate effective protection against challenge following immunization with the CEK-adapted virus.…”

Section: Discussionmentioning

confidence: 68%

“…The virus population shifted towards homogeneity in spike (S) and nonstructural (NSP) genes during seven passages in CEK cells. Based on S gene sequencing, the changes of the predominant Ark population after CEK adaptation did not revert after one back-passage in embryonating chicken eggs nor after a passage in chickens (6). Because of the advantages of this more stable and homogeneous CEK-adapted ArkDPI virus, this study was aimed at evaluating its ability to confer protection against homologous challenge.…”

mentioning

confidence: 99%

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Kidney Cell–Adapted Infectious Bronchitis Virus Arkansas Delmarva Poultry Industry Vaccine Confers Effective Protection Against Challenge

et al. 2016

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We previously demonstrated that adaptation of an embryo-attenuated infectious bronchitis virus (IBV) Arkansas Delmarva Poultry Industry (ArkDPI)-derived vaccine to chicken embryo kidney (CEK) cells shifted the virus population towards homogeneity in spike (S) and nonstructural protein genes. Moreover, the typical Ark vaccine subpopulations emerging in chickens vaccinated with commercial Ark vaccines were not detected in chickens vaccinated with the CEK-adapted virus. In this study, chickens vaccinated with a low dose (1.6 × 10(3) EID50/bird, where EID50 is 50% embryo infectious dose) of CEK-adapted Ark vaccine at 5 days of age showed a significant reduction of IBV RNA in lachrymal fluids and decreased incidence of IBV RNA detection in tracheal swabs 5 days after challenge compared to unvaccinated challenged chickens. In a second experiment, 5-day-old chickens were vaccinated with 10(4) or 10(5) EID50/chicken of CEK-adapted Ark vaccine, and protection was compared to chickens vaccinated with 10(5) EID50/chicken of the commercial ArkDPI-derived vaccine from which the CEK-adapted virus originated. All vaccinated chicken groups showed a significant reduction of respiratory signs and viral load 5 days after Ark virulent challenge compared to unvaccinated challenged controls. No viral subpopulations different from the challenge virus were detected in chickens vaccinated with CEK-Ark after challenge. In contrast, IBV S1 sequences differing from the predominant population in the challenge virus were detected in several chickens vaccinated with the commercial Ark attenuated vaccine. From an applied perspective, the CEK-adapted IBV ArkDPI-derived vaccine is an improved and effective vaccine candidate with which to protect chickens against virulent Ark-type strains.

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Section: Discussionmentioning

confidence: 68%

mentioning

confidence: 99%

Kidney Cell–Adapted Infectious Bronchitis Virus Arkansas Delmarva Poultry Industry Vaccine Confers Effective Protection Against Challenge

et al. 2016

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“…Viruses. The previously described CEK-adapted ArkDPI vaccine virus (CEKp7), which had been back passaged once in SPF ECE (7) was subjected to four further back passages in SPF ECE. The second passage was used at two dose levels to assess protection conferred by vaccination (see experimental designs below).…”

Section: Methodsmentioning

confidence: 99%

“…An average of 7508 reads were evaluated at each genome position. Frequencies of nucleotides at each genome position were compared with those previously obtained from the IBV vaccine virus stock and from the CEKp7 after 1 ECE back passage (7).…”

Section: Methodsmentioning

confidence: 99%

“…The virus population shifted toward homogeneity in spike (S) and nonstructural (NSP) genes during passages in CEK cells. Based on S gene sequencing, the changes of the predominant Ark population after CEK adaptation were not reverted after one back passage in embryonated chicken eggs (ECE) or after a passage in chickens (7). Because of the advantages of this more homogeneous CEK-adapted ArkDPI virus, this study was aimed at evaluating its stability during further back passages in ECE, and its ability to confer protection against homologous challenge.…”

mentioning

confidence: 99%

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Kidney Cell–Adapted Infectious Bronchitis ArkDPI Vaccine is Stable and Protective

et al. 2017

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We previously demonstrated that adaptation of an embryo-attenuated infectious bronchitis virus (IBV) Arkansas (Ark) Delmarva Poultry Industry (DPI)-derived vaccine to chicken embryo kidney (CEK) cells (CEKp7) shifted the virus population towards homogeneity in spike (S) and nonstructural protein genes. Moreover, the typical Ark vaccine subpopulations emerging in chickens vaccinated with commercial Ark vaccines were not detected in chickens vaccinated with CEKp7, indicating that kidney-cell adaptation drastically increased the stability of the vaccine virus population in chickens. In the current study both conventional and next-generation sequencing results show that the changes achieved during CEK adaptation remained after five back passages in embryonated chicken egg (ECE). In a first protection study 1-day-old chickens were vaccinated with 10 or 10 50% embryo infectious doses (EID)/chicken of the second ECE back passage of CEKp7 (CEKp7e2) and demonstrated protection against Ark virulent (10 EID) challenge. In a second protection trial, protection by CEKp7e2 was compared with protection conferred by an attenuated commercial ArkDPI-derived vaccine different from that which the CEK-adapted virus originated. All vaccinated chicken groups showed a significant reduction of respiratory signs and viral load after Ark virulent challenge compared to unvaccinated-challenged controls. In CEKp7e2 vaccinated chickens viral subpopulations different from the challenge virus were detected after challenge in a marginal number (7%-8%) of chickens. In contrast, IBV S1 sequences that differed from the predominant population in the challenge virus were detected after challenge in a large number (77%) of chickens vaccinated with the commercial Ark attenuated vaccine. The CEK-adapted IBV ArkDPI-derived vaccine is a stable and effective vaccine, which drastically reduces the emergence of Ark-like viruses both at vaccination and after challenge.

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Selenium deficiency aggravates bisphenol A‐induced autophagy in chicken kidney through regulation of nitric oxide and adenosine monophosphate activated protein kinase/mammalian target of rapamycin signaling pathway

Chen

Zhang

et al. 2022

Environmental Toxicology

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Bisphenol A (BPA), a phenolic compound, is harmful to humans and animals as its residue in the water threatens multiple organs, especially the kidney. Low selenium (Se) diets are consumed in many regions of the world, and poor Se status has exacerbating effect on toxicity of several environmental chemicals. Here, we described the discovery path of Se deficiency aggravation on autophagy in BPA treated chicken kidney through regulating nitric oxide (NO) and adenosine monophosphate activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) signaling pathways.The actual dietary Se intake for chickens was 0.30 mg/kg in control group and 0.03 mg/kg in Low-Se group, and BPA exposure concentration for chickens was 0.05 g/kg. Chicken embryo kidney (CEK) cells were used in vitro and the BPA exposure concentration for CEK cells was 150 nM. We found that BPA significantly increased levels of NO and inducible nitric oxide synthase, activated AMPK/mTOR signaling pathways, thereby triggering p62/LC3/Beclin1 signaling, resulting in formations of autophagosome and autolysosome, and finally stimulating autophagy in the chicken kidney. Additionally, Se deficiency promoted the occurrence of autophagy in BPA-treated kidneys. Altogether, our findings showed that Se deficiency exacerbates BPA-induced renal autophagy in chickens via regulation of NO and AMPK/mTOR signaling pathways. These findings will improve our understandings of the mechanisms of nephrotoxicity of BPA and detoxification by Se in chickens. In addition, further work is required to determine if Se status of exposed populations needs to be considered in future epidemiological assessments.

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Effects of Adaptation of Infectious Bronchitis Virus Arkansas Attenuated Vaccine to Embryonic Kidney Cells

Cited by 18 publications

References 38 publications

Kidney Cell–Adapted Infectious Bronchitis Virus Arkansas Delmarva Poultry Industry Vaccine Confers Effective Protection Against Challenge

Kidney Cell–Adapted Infectious Bronchitis Virus Arkansas Delmarva Poultry Industry Vaccine Confers Effective Protection Against Challenge

Kidney Cell–Adapted Infectious Bronchitis ArkDPI Vaccine is Stable and Protective

Selenium deficiency aggravates bisphenol A‐induced autophagy in chicken kidney through regulation of nitric oxide and adenosine monophosphate activated protein kinase/mammalian target of rapamycin signaling pathway

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