Effects of Acute Lymphoblastic Leukemia on Ceruloplasmin Oxidase, Copper and Several Markers of Oxidative Damage, in Children

Mehdi, Wesen Adel; Yusof, Farida Zuraina Mohd; Mehde, Atheer Awad; Zainulabdeen, Jwan A.; Raus, Raha Ahmad; Abdulbari, Alaa Shawqi

doi:10.7314/apjcp.2015.16.13.5205

Cited by 10 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, enzymes can also undergo changes in their conformation, together with changes in pH [ 38 ]. The reduction in CP oxidase activity at low pH may be due to the effect of the pH of the analytical mixture in ionic groups on the active site, or variation in the ionic state of the substrate or enzyme–substrate complex [ 39 ]. The optimum pH for CP oxidative activity assessment depends not only on the nature of the chemical substrate, but also on the different animal species.…”

Section: Discussionmentioning

confidence: 99%

“…The optimum pH for CP oxidative activity assessment depends not only on the nature of the chemical substrate, but also on the different animal species. Thus, for example, in humans, the optimal pH is 5.4, but at pH 4.6, enzymatic activity is already inhibited by 50–80% [ 39 ]. In dogs, the best analytical condition is obtained at pH 5.2, whereas at pH 4.8, CP oxidase activity decreases by about 10–20% [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ceruloplasmin Interferes with the Assessment of Blood Lipid Hydroperoxide Content in Small Ruminants

Gualandi

Boni

2023

Antioxidants

View full text Add to dashboard Cite

Simple and inexpensive analytical methods for assessing redox balance in biological matrixes are widely used in animal and human diagnostics. Two of them, reactive oxygen metabolites (ROMs) and total oxidant status (TOS), evaluate the lipid hydroperoxide (LOOH) content of the sample and are based on iron-mediated mechanisms. However, these tests provide uncorrelated results. In this study, we compared these two tests in the blood serum of goat kids and lambs, together with an evaluation of ceruloplasmin (CP) oxidase activity. No significant correlation was found between ROMs and TOS, or between TOS and CP oxidase activity, in either species. Conversely, ROMs and CP oxidase activity were highly correlated in both kid and lamb samples (p < 0.001). A significant progressive reduction in the analytical signal in the ROMs assay was observed when sodium azide, an effective CP inhibitor, was added to the samples before the assay (p < 0.001). This decrease was related to sodium azide concentration (p < 0.01) and was not found when sodium azide was added at the same concentrations in the TOS assay. These findings suggest that ROMs, unlike TOS, may be affected by CP, which interferes with LOOH detection in blood samples.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%