Effects of Acute Hypoxic Stress on Physiological and Hepatic Metabolic Responses of Triploid Rainbow Trout (Oncorhynchus mykiss)

Han, Buying; Meng, Yuqiong; Tian, Haining; Li, Changzhong; Li, Yaopeng; Gongbao, Caidan; Fan, Wei; Ma, Rui

doi:10.3389/fphys.2022.921709

Cited by 14 publications

(12 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Also, plasma glucose and lactate were increased at 1 h sampling time, showing a positive correlation with cortisol and recovering basal values 6 h after hypoxia. Similar results were observed in triploid rainbow trout by Han et al (2022), in turbot (Jia et al, 2021), and in rainbow trout exposed to acute stress hypoxia (4 mg O 2 /L) and crowding within 10 min (Pinedo-Gil et al, 2019). The absence of time effect on glycemic levels after hypoxia could be due to the fact that the stressor was not intense enough to induce sufficient gluconeogenesis and glycogenolytic action, or to the fact that one of the mediators of such response, catecholamines, shows a very quick response (Weendelar-Bonga, 1997), which did not help to detect significant changes at the sampling time assessed.…”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

Rainbow trout integrated response after recovery from short-term acute hypoxia

2022

View full text Add to dashboard Cite

Overcoming a stress situation, such as hypoxia episodes, which involve an allostatic load, will depend on the ability of fish to modulate physiological and biochemical systems to maintain homeostasis. The aim of the study was to determine the integrated stress response after acute hypoxia of the rainbow trout considering the different elements and areas of the stress response: systemic and mucosal, local and global, and from the systemic hypothalamic–pituitary–interrenal axis to skin mucosa. For this purpose, trout were subjected to acute hypoxia (dissolved O2 down to 2 mg/L) for 1 h and then recovered and sampled at 1, 6, and 24 h after reoxygenation. Physiological responses were significantly affected by hypoxic stress and their interaction with time after the challenge, being significant for plasma lactate and cortisol levels, in both plasma and skin mucus. At the central brain level, only trh expression was modulated 1 h after hypoxia which indicates that brain function is not heavily affected by this particular stress. Unlike the brain, the head kidney and skin were more affected by hypoxia and reoxygenation. In the head kidney, an upregulation in the expression of most of the genes studied (gr, il1β, il6, tgfβ1, lysozyme, caspase 3, enolase, hif-1, myoglobin, sod2, gpx, gst, and gsr) took place 6 h after recovery, whereas only hsp70 and il10 were upregulated after 1 h. On the contrary, in the skin, most of the analyzed genes showed a higher upregulation during 1 h after stress suggesting that, in the skin, a local response took place as soon as the stressor was detected, thus indicating the importance of the skin in the building of a stress response, whereas the interrenal tissue participated in a later time point to help prevent further alteration at the central level. The present results also show that, even though the stressor is a physical/environmental stressor, all components of the biological systems participate in the regulation of the response process and the recovery process, including neuroendocrine, metabolism, and immunity.

show abstract

Section: Discussionsupporting

confidence: 87%

“…On the contrary, Han et al (2022) found that sod, catalase, and gpx transcript levels in the liver from triploid rainbow trout were downregulated or unchanged after 10 min under hypoxia and crowding. These differences during hypoxia-reoxygenation could be due to species-, tissue-, and time-specificity, thus resulting in different dynamics.…”

Section: Discussionmentioning

confidence: 86%

Rainbow trout integrated response after recovery from short-term acute hypoxia

2022

View full text Add to dashboard Cite

show abstract

“…Cells in living organisms adapt to hypoxia by changing metabolism, which is facilitated by changes in protein expression, mRNA or protein stability that occur at the level of transcription or translation (Solaini et al, 2010;. Multiple studies have clearly shown that oxygen homeostasis and iron metabolism are interlinked, such as the target of HIF, which play an important role in cell adaptation to low oxygen levels in normal and Frontiers in Physiology frontiersin.org damaged tissues, are involved in iron homeostasis (Shah and Xie, 2014;Xu et al, 2017;Yang et al, 2018;Han et al, 2022). The regulation between hypoxia and iron is complexed.…”

Section: Discussionmentioning

confidence: 99%

Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death

et al. 2022

Front. Physiol.

View full text Add to dashboard Cite

Acute hypoxia in water has always been a thorny problem in aquaculture. Oxygen and iron play important roles and are interdependent in fish. Iron is essential for oxygen transport and its concentration tightly controlled to maintain the cellular redox homeostasis. However, it is still unclear the role and mechanism of iron in hypoxic stress of fish. In this study, we investigated the role of iron in hypoxic responses of two zebrafish-derived cell lines. We found hypoxia exposed zebrafish liver cells (ZFL) demonstrated reduced expression of Ferritin and the gene fth31 for mitochondrial iron storage, corresponding to reduction of both intracellular and mitochondrial free iron and significant decrease of ROS levels in multiple cellular components, including mitochondrial ROS and lipid peroxidation level. In parallel, the mitochondrial integrity was severely damaged. Addition of exogenous iron restored the iron and ROS levels in cellular and mitochondria, reduced mitochondrial damage through enhancing mitophagy leading to higher cell viability, while treated the cells with iron chelator (DFO) or ferroptosis inhibitor (Fer-1) showed no improvements of the cellular conditions. In contrast, in hypoxia insensitive zebrafish embryonic fibroblasts cells (ZF4), the expression of genes related to iron metabolism showed opposite trends of change and higher mitochondrial ROS level compared with the ZFL cells. These results suggest that iron homeostasis is important for zebrafish cells to maintain mitochondrial integrity in hypoxic stress, which is cell type dependent. Our study enriched the hypoxia regulation mechanism of fish, which helped to reduce the hypoxia loss in fish farming.

show abstract

“…Fish fed the diet with the 6.6% lipid level had higher a-i HYP and total HYP contents than fish fed the diets with 14.8%, 22.8% and 29.4% (p < 0.05), while a-s HYP and PYD contents were not affected by dietary lipid levels (p > 0.05). 0.12 ± 0.01 0.10 ± 0.01 0.13 ± 0.01 0.14 ± 0.01 a-i HYP (mg/g muscle) 1 0.23 ± 0.02 a 0.01 ± 0.01 b 0.01 ± 0.00 b 0.01 ± 0.00 b Total HYP (mg/g muscle) 1 0.32 ± 0.03 a 0.11 ± 0.01 b 0.14 ± 0.01 b 0.15 ± 0.01 b PYD (μg/g muscle) 1 2.08 ± 0.11 2.12 ± 0.08 2.00 ± 0.14 2.03 ± 0.09…”

Section: Fillet Texturementioning

confidence: 99%

“…Rainbow trout (Oncorhynchus mykiss) is a globally farmed species. To avoid the degradation of fish quality associated with sexual maturation, triploid rainbow trout has been the main cold-water fish species cultured in China [1]. With the increase in production, fillet quality has become an important factor to determine consumer preference and sustainable development of aquaculture for the fish.…”

Section: Introductionmentioning

confidence: 99%

Does Dietary Lipid Level Affect the Quality of Triploid Rainbow Trout and How Should It Be Assessed?

Meng

Liu

Guan

et al. 2022

Foods

Self Cite

View full text Add to dashboard Cite

Organoleptic properties and nutritional value are the most important characteristics of fish fillet quality, which can be determined by a series of quality evaluation indexes and closely related to fish nutrition. Systematic organoleptic and nutritional quality evaluation indexes consisting of 139 indexes for physical properties and chemical compositions of triploid rainbow trout were established. Besides, effects of dietary lipid levels (6.6%, 14.8%, 22.8% and 29.4%) on the quality of triploid rainbow trout were analyzed in the study. The main results showed that, for fillet appearance quality, fish fed diets with lipid levels above 22.8% had higher fillet thickness and redness but lower gutted yield and fillet yield (p < 0.05). For fillet texture, fish fed the diet with a 6.6% lipid level had the highest fillet hardness (5.59 N) and lowest adhesiveness (1.98 mJ) (p < 0.05), which could be related to lipid, glycogen, water soluble protein and collagen contents of the fish fillet. For fillet odor, the odor intensity of “green, fatty and fishy” significantly increased with the increase of the dietary lipid level (from 1400 to 2773 ng/g muscle; p < 0.05), which was related to the degradation of n-6 and n-9 fatty acids. For fillet taste, a high lipid diet (≥22.8%) could increase the umami taste compounds contents (from 114 to 261 mg/100 g muscle) but decrease the bitterness and sourness taste compounds contents (from 127 to 106 mg/100 g muscle and from 1468 to 1075 mg/100 g muscle, respectively) (p < 0.05). For nutritional value, a high lipid diet could increase the lipid nutrition level (such as the content of long chain polyunsaturated fatty acids increased from 3.47 to 4.41 g/kg muscle) but decease tryptophan and selenium content (from 2.48 to 1.60 g/kg muscle and from 0.17 to 0.11 g/kg muscle, respectively). In total, a high lipid diet could improve the quality of triploid rainbow trout. The minimum dietary lipid level for triploid rainbow trout should be 22.8% to keep the better organoleptic and nutritional quality.

show abstract

Effects of Acute Hypoxic Stress on Physiological and Hepatic Metabolic Responses of Triploid Rainbow Trout (Oncorhynchus mykiss)

Cited by 14 publications

References 52 publications

Rainbow trout integrated response after recovery from short-term acute hypoxia

Rainbow trout integrated response after recovery from short-term acute hypoxia

Iron supplementation inhibits hypoxia-induced mitochondrial damage and protects zebrafish liver cells from death

Does Dietary Lipid Level Affect the Quality of Triploid Rainbow Trout and How Should It Be Assessed?

Contact Info

Product

Resources

About