Effects of Acute Ethanol Administration on Brain Oxidative Status: The Role of Acetaldehyde

Baliño, Pablo; Romero-Cano, Ricard; Sánchez-Andrés, Juan Vicente; Valls, Victoria; Aragón, Carlos González; Muriach, Marí­a

doi:10.1111/acer.14133

Cited by 11 publications

(9 citation statements)

References 85 publications

(113 reference statements)

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“…An important goal for future studies is to assess more accurate blood alcohol levels early in the operant session in both male and female rats while also taking into account the detrimental effects of alcohol metabolites in the brain (Baliño et al, 2019); reviewed in (Erol and Karpyak, 2015;Israel et al, 2015).…”

Section: Blood Alcohol Levelsmentioning

confidence: 99%

Shortening Time for Alcohol Access Drives Up Front-Loading Behavior, Bringing Consumption in Male Rats to the Level of Females

Flores‐Bonilla

Oliveira

Silva-Gotay

et al. 2021

Preprint

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Alcohol can have more detrimental effects on mental health in women, even when intake is comparable or higher in men. This may relate to a differential pattern of drinking, e.g., how rapidly alcohol is consumed. We used operant procedures to gain insight into sex differences in the drinking dynamics of rats. Adult male and female Wistar rats underwent operant training to promote voluntary drinking of 10% (w/v) alcohol (8 rats/sex). We tested how drinking patterns changed after manipulating the effort required for alcohol (fixed ratio, FR), as well as the length of time in which animals had access to alcohol (self-administration session length). Rats were tested twice within the 12 hours of the dark cycle, at 2 hours (early sessions) and 10 hours into the dark cycle (late sessions). As expected, adult females consumed significantly more alcohol than males in the 30-minute sessions with the FR1 paradigm. Alcohol consumption within females was higher in the late sessions compared to early sessions, whereas this difference was not found within males. Front-loading of alcohol (heavier drinking in the first five minutes of the session) was the primary factor underlying higher consumption in females, and this sex difference was accentuated in the late sessions. Increasing the effort required from FR1 to FR3 reduced alcohol drinking in both sexes. Front-loading behavior remained in females in both early and late sessions, whereas males exhibited minimal front-loading behavior only in the early sessions. Compressing drinking access to 15-minutes drove up front-loading behavior, producing total alcohol intake levels that were comparable in both sexes. This strategy could be useful for exploring sex differences in the effect of voluntary alcohol drinking on the brain. Our findings also highlight the importance of the time of testing for detecting sex differences in drinking behavior.

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Section: Blood Alcohol Levelsmentioning

confidence: 99%

Shortening Time for Alcohol Access Drives Up Front-Loading Behavior, Bringing Consumption in Male Rats to the Level of Females

Flores‐Bonilla

Oliveira

Silva-Gotay

et al. 2021

Preprint

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“…Oxidative metabolism of ethanol persuades ROS production and OS. Ethanol exposure causes membrane lipid peroxidation [ 54 ], depletion of glutathione, and formation of protein carbonyl that serve as markers of OS in the brain [ 55 ]. Several dopaminergic neurons are engaged in ethanol-related impairments in mammalian CNS as well as in Drosophila [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

α-Synuclein E46K Mutation and Involvement of Oxidative Stress in a Drosophila Model of Parkinson’s Disease

Jahromi

Ramesh

Finkelstein³

et al. 2021

Parkinson's Disease

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Parkinson’s disease (PD) is an age-associated neurodegenerative condition in which some genetic variants are known to increase disease susceptibility on interaction with environmental factors inducing oxidative stress. Different mutations in the SNCA gene are reported as the major genetic contributors to PD. E46K mutation pathogenicity has not been investigated as intensive as other SNCA gene mutations including A30P and A53T. In this study, based on the GAL4-UAS binary genetic tool, transgenic Drosophila melanogaster flies expressing wild-type and E46K-mutated copies of the human SNCA gene were constructed. Western blotting, immunohistochemical analysis, and light and confocal microscopy of flies’ brains were undertaken along with the survival rate measurement, locomotor function assay, and ethanol and paraquat (PQ) tolerance to study α-synuclein neurotoxicity. Biochemical bioassays were carried out to investigate the activity of antioxidant enzymes and alterations in levels of oxidative markers following damages induced by human α-synuclein to the neurons of the transgenic flies. Overexpression of human α-synuclein in the central nervous system of these transgenic flies led to disorganized ommatidia structures and loss of dopaminergic neurons. E46K α-synuclein caused remarkable climbing defects, reduced survivorship, higher ethanol sensitivity, and increased PQ-mediated mortality. A noticeable decline in activity of catalase and superoxide dismutase enzymes besides considerable increase in the levels of lipid peroxidation and reactive oxygen species was observed in head capsule homogenates of α-synuclein-expressing flies, which indicates obvious involvement of oxidative stress as a causal factor in SNCAE46K neurotoxicity. In all the investigations, E46K copy of the SNCA gene was found to impose more severe defects when compared to wild-type SNCA. It can be concluded that the constructed Drosophila models developed PD-like symptoms that facilitate comparative studies of molecular and cellular pathways implicated in the pathogenicity of different α-synuclein mutations.

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“…(1,5) The normal level of homeostasis of laboratory animals and humans is maintained at blood concentrations of EE of about 0.05-0.2 mM, and EA is about 0.3-0.8 μM. The biological role of EE is diverse: (1) is a high-energy compound and under normal conditions can provide up to 10% of the body's energy needs; (2) participates in the maintenance of the liquid-crystalline, fluid state of the lipid layer of membranes, "liquefying" (fluidizing) them; (11)(12)(13) (3) is a regulator of lipid peroxidation in cell membranes, showing properties of a free radical trap and activating cholesterol synthesis. (11) EA is chemically very active, does not penetrate cell membranes, but can change their permeability to other substances.…”

Section: Adh2mentioning

confidence: 99%

“…(2) regulatory modification (through the formation of Schiff bases) of opioid peptides; (3) with the participation of EA, endogenous morphine and morphine-like compounds are synthesized; (4) regulates the exchange of the most important neurochemical mediators of the amine nature: dopamine, norepinephrine, serotonin; hormone adrenaline. (12)(13)(14) Everything discussed above allows us to consider the EE-EA system as an important element of non-specific regulatory systems of the body, providing the body's adaptive capabilities to the effects of stress, including cold.…”

Section: Adh2mentioning

confidence: 99%

“…The mechanism of the cold-adaptive action of EE apparently consists, first, in its ability to easily penetrate into all cells of the body, reduce the viscosity of the lipid layer of cell membranes and optimize functioning at lower temperatures of membrane tissue-specific, transport and receptor complexes. (6,8,9,13) It consists second in the easy and fast mobilization of EE, primarily as a catabolic substrate; therefore, its concentration in the blood at the first stage of adaptation to cold decreases. However, on Day 28 of cold stress, the EE level is not only restored, but increases in overcompensation mode by 3.4-5.0 times.…”

mentioning

confidence: 99%

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Cold Stress and Endogenic System Ethanol – Acetaldehyde

Kolosova¹,

Kerschengolts²,

Khlebnyy³

2019

IJBM

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The article presents data on the effects of cold stress on changes in the concentrations of endogenous ethanol (EE) and acetaldehyde (EA), and the activities of the key enzymes involved in their metabolism-alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (AHD) in laboratory animals. Mature male Wistar rats were used in the experiment. Animals in the vivarium were kept in cages at a temperature of 20°-22°C, without limitation of mobility, with free access to water with an adjustable light schedule (12 hours-light, 12 hours-darkness). Simulation of cold stressing was carried out for 7 weeks at a temperature of 1-2°C, starting from 0.5 hour to 6 hours per day by the fourth week of the experiment. The concentration of EE and EA was determined by gas chromatography-mass spectrometry (GC-MS). The analysis of the activity of the ADH and AHD enzymes was performed by standard spectrophotometric methods. The experiment showed that during cold stresses, the survival rate in rats strongly correlates with the EE content in the blood (r=0.757÷0.923). This fact suggests that when adapting cold-adapted animals to the effects of low temperatures, it is important to increase the EE concentration in the body, and the consumption of EE compensates for the increased need of the body in cold conditions for this metabolite. Based on the data obtained, a probable mechanism of the participation of EE in the processes of adaptation of higher vertebrates to cold is presented, which includes specific adaptive changes in the activities of the ADH and AHD enzymes.

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Effects of Acute Ethanol Administration on Brain Oxidative Status: The Role of Acetaldehyde

Cited by 11 publications

References 85 publications

Shortening Time for Alcohol Access Drives Up Front-Loading Behavior, Bringing Consumption in Male Rats to the Level of Females

Shortening Time for Alcohol Access Drives Up Front-Loading Behavior, Bringing Consumption in Male Rats to the Level of Females

α-Synuclein E46K Mutation and Involvement of Oxidative Stress in a Drosophila Model of Parkinson’s Disease

Cold Stress and Endogenic System Ethanol – Acetaldehyde

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