Effects of acute change in salinity and moulting on the infection of white leg shrimp (<i>Penaeus vannamei</i>) with white spot syndrome virus upon immersion challenge

Thuong, Khuong Van; Tuan, Vo Van; Li, W; Sorgeloos, Patrick; Bossier, Peter; Nauwynck, Hans

doi:10.1111/jfd.12471

Cited by 16 publications

(8 citation statements)

References 53 publications

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“…Results showed that expression trend of those genes in L . maculates liver were in general agreement with previously reports on these gene’s roles in fishes’ osmoregulatory organs with one exception, aqp3 expression has been found decreased in response to high salinity challenge in many teleost species [ 51 – 53 ], such a discrepancy may result from the different studied species and tissues.…”

Section: Discussionsupporting

confidence: 91%

RNA-Seq analysis of salinity stress–responsive transcriptome in the liver of spotted sea bass (Lateolabrax maculatus)

et al. 2017

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Salinity is one of the most prominent abiotic factors, which greatly influence reproduction, development, growth, physiological and metabolic activities of fishes. Spotted sea bass (Lateolabrax maculatus), as a euryhaline marine teleost, has extraordinary ability to deal with a wide range of salinity changes. However, this species is devoid of genomic resources, and no study has been conducted at the transcriptomic level to determine genes responsible for salinity regulation, which impedes the understanding of the fundamental mechanism conferring tolerance to salinity fluctuations. Liver, as the major metabolic organ, is the key source supplying energy for iono- and osmoregulation in fish, however, little attention has been paid to its salinity-related functions but which should not be ignored. In this study, we perform RNA-Seq analysis to identify genes involved in salinity adaptation and osmoregulation in liver of spotted sea bass, generating from the fishes exposed to low and high salinity water (5 vs 30ppt). After de novo assembly, annotation and differential gene expression analysis, a total of 455 genes were differentially expressed, including 184 up-regulated and 271 down-regulated transcripts in low salinity-acclimated fish group compared with that in high salinity-acclimated group. A number of genes with a potential role in salinity adaptation for spotted sea bass were classified into five functional categories based on the gene ontology (GO) and enrichment analysis, which include genes involved in metabolites and ion transporters, energy metabolism, signal transduction, immune response and structure reorganization. The candidate genes identified in L. maculates liver provide valuable information to explore new pathways related to fish salinity and osmotic regulation. Besides, the transcriptomic sequencing data supplies significant resources for identification of novel genes and further studying biological questions in spotted sea bass.

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Section: Discussionsupporting

confidence: 91%

RNA-Seq analysis of salinity stress–responsive transcriptome in the liver of spotted sea bass (Lateolabrax maculatus)

et al. 2017

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“…The virus is known to occur in fresh, brackish, and marine water. WSSV multiplies due to environmental stress, temperature, pH, salinity, plankton blooming, molting, and spawning [[2], [3], [4]]. To prevent WSSV infection, several chemicals have been used particularly formaldehyde, malachite green, calcium oxide, and sodium hypochlorite [5]; but these chemicals pose environmental hazards.…”

Section: Introductionmentioning

confidence: 99%

Screening and selection of bacteria inhibiting white spot syndrome virus infection to Litopenaeus vannamei

Sekar

Kim

Jeon³

et al. 2019

Biochemistry and Biophysics Reports

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A total of 173 bacterial strains were isolated from different sources at different regions such as fermented foods, shrimp guts, sea water, mangrove water, and sediments. These bacteria were screened against white spot syndrome virus (WSSV) infection in Palaemon paucidens . Based on mortality, white spot level, and healthiness, three bacterial strains were selected and identified using 16S rRNA gene sequencing. These bacterial strains were Bacillus subtilis KA1, B. licheniformis KA2, and B. subtilis KA3. WSSV challenge test in pilot scale was conducted using Litopenaeus vannamei with B. subtilis KA1 and B. subtilis KA3. The survival ratio of shrimp was 0% for WSSV control after 17th days, 84% for B. subtilis KA1 plus WSSV after 26th days, and 28% for B. subtilis KA3 with WSSV after 26th days. B. subtilis KA1 showed good growth at 18–37 °C in with and without 3% NaCl, and therefore can be applied to aquaculture at low to high temperatures. B. subtilis KA1 produced protease and lipase which can increase digestion to shrimp; exhibited antibacterial activity against Vibrio parahaemolyticus ; and significantly increased the survival of WSSV challenged shrimps.

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“…Outbreaks of white spot syndrome virus are common in shrimp farms during the rainy season, with a possible association with lower salinity and temperatures. It has recently been reported that shrimp are more susceptible to white spot syndrome virus when the water is at a lower salinity, suggesting that chloride ions may have an antiviral effect in marine organisms as well 23 . This potential relationship between virus, eukaryotic co-evolution and antiviral mechanism is interesting and needs further research.…”

Section: Discussionmentioning

confidence: 99%

Antiviral innate immune response in non-myeloid cells is augmented by chloride ions via an increase in intracellular hypochlorous acid levels

Ramalingam

Cai

Wong

et al. 2018

Sci Rep

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Phagocytes destroy ingested microbes by producing hypochlorous acid (HOCl) from chloride ions (Cl−) and hydrogen peroxide within phagolysosomes, using the enzyme myeloperoxidase. HOCl, the active ingredient in bleach, has antibacterial/antiviral properties. As myeloperoxidase is needed for HOCl production, non-myeloid cells are considered incapable of producing HOCl. Here, we show that epithelial, fibroblast and hepatic cells have enhanced antiviral activity in the presence of increasing concentrations of sodium chloride (NaCl). Replication of enveloped/non-enveloped, DNA (herpes simplex virus-1, murine gammaherpesvirus 68) and RNA (respiratory syncytial virus, influenza A virus, human coronavirus 229E, coxsackievirus B3) viruses are inhibited in a dose-dependent manner. Whilst treatment with sodium channel inhibitors did not prevent NaCl-mediated virus inhibition, a chloride channel inhibitor reversed inhibition by NaCl, suggesting intracellular chloride is required for antiviral activity. Inhibition is also reversed in the presence of 4-aminobenzoic hydrazide, a myeloperoxidase inhibitor, suggesting epithelial cells have a peroxidase to convert Cl− to HOCl. A significant increase in intracellular HOCl production is seen early in infection. These data suggest that non-myeloid cells possess an innate antiviral mechanism dependent on the availability of Cl− to produce HOCl. Antiviral activity against a broad range of viral infections can be augmented by increasing availability of NaCl.

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Effects of acute change in salinity and moulting on the infection of white leg shrimp (Penaeus vannamei) with white spot syndrome virus upon immersion challenge

Cited by 16 publications

References 53 publications

RNA-Seq analysis of salinity stress–responsive transcriptome in the liver of spotted sea bass (Lateolabrax maculatus)

RNA-Seq analysis of salinity stress–responsive transcriptome in the liver of spotted sea bass (Lateolabrax maculatus)

Screening and selection of bacteria inhibiting white spot syndrome virus infection to Litopenaeus vannamei

Antiviral innate immune response in non-myeloid cells is augmented by chloride ions via an increase in intracellular hypochlorous acid levels

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