Effects of acute cartilaginous injury on serum and cartilage lysozyme levels

Greenwald, Robert A.; Cantor, Jerome O.; Schwartz, Charles E.; Josephson, Alan S.

doi:10.1002/art.1780180211

Cited by 6 publications

(1 citation statement)

References 17 publications

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“…In cartilage, for example, lysozyme is present as a non-lysosomal matrix protein capable of interacting with surrounding polyanionic tissue components. Furthermore, it has been shown that injury to cartilage results in a parallel release of lysozyme and chondroitin sulphate, indicating a possible biochemical relationship between these two constituents 6. Since neither of these matrix components is specific to cartilage, similar interactions could occur in other tissues.…”

Section: Discussionmentioning

confidence: 99%

Preferential binding of lysozyme to elastic fibres in pulmonary emphysema

Shteyngart

Chaiwiriyakul

Wong

et al. 1998

Thorax

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Background-Lysozyme is increased in inflammatory reactions and is a component of the extracellular matrix, but its possible role in lung diseases such as emphysema and interstitial fibrosis has not been investigated. Methods-To characterise diVerences in lysozyme content among normal, emphysematous, and fibrotic human lungs, tissue sections obtained from necropsy specimens were immunostained with rabbit polyclonal anti-human lysozyme antibody using the labelled streptavidin-biotin peroxidase method. The immunostained sections were evaluated semiquantitatively (grading the degree of immunostaining on a scale of 0-4). To determine if degradation of the extracellular matrix aVects lysozyme binding, hyaluronidase-treated normal lung tissues were incubated with egg white lysozyme, immunostained with the lysozyme antibody, which crossreacts with egg white lysozyme, and evaluated for degree of staining. Results-Lysozyme immunostaining was significantly increased in lungs with pulmonary emphysema compared with normal or fibrotic tissues (3.4 versus 1.6 and 1.9, respectively; p<0.05) and was preferentially associated with interstitial elastic fibres. Hyaluronidase-treated lung tissues incubated with lysozyme showed increased immunostaining for this protein compared with untreated controls (1.9 versus 1.2; p<0.05). Conclusions-The results suggest that damage to elastic fibres and/or the surrounding extracellular matrix increases lysozyme binding. It is hypothesised that attachment of lysozyme to elastic fibres may interfere with their repair and possibly enhance the progression of pulmonary emphysema. (Thorax 1998;53:193-196) Keywords: lysozyme; pulmonary emphysema; elastic fibres This study examined the possible role of lysozyme in lung injury. The investigation was prompted by earlier studies indicating that lysozyme is increased in a number of inflammatory conditions and is specifically associated with extracellular matrix constituents.1-3 These findings suggested that the amount of lysozyme in the lung might be altered in diseases such as emphysema or interstitial fibrosis where there is damage to various matrix components.To determine if lysozyme is involved in pulmonary disease, tissue sections from normal, fibrotic, and emphysematous human lungs were evaluated for diVerences in lysozyme content. An increase in extracellular lysozyme was specifically observed in lung tissues with pulmonary emphysema, and the protein was preferentially associated with elastic fibres which undergo breakdown in this disease. Since this laboratory and other investigators have previously shown that hyaluronic acid and other polysaccharides surround elastic fibres, 4 5 normal lung tissues were treated with hyaluronidase and examined for their ability to bind exogenously administered lysozyme. Such treatment resulted in increased attachment of lysozyme, suggesting that degradation of extracellular matrix components, as occurs in pulmonary emphysema, may expose binding sites for lysozyme on elastic fibres, possibly altering matri...

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Section: Discussionmentioning

confidence: 99%

Preferential binding of lysozyme to elastic fibres in pulmonary emphysema

Shteyngart

Chaiwiriyakul

Wong

et al. 1998

Thorax

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Lactoferrin and lysozyme levels in synovial fluid

Bennett

Skosey

1977

Arthritis & Rheumatism

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Lysozyme and lactoferrin levels were measured in 71 synovial fluids (SF) of patients with traumatic effusions, osteoarthritis, rheumatoid arthritis, pseudogout, septic arthritis, and gout, as well as in 91 synovial fluids graded according to their neutrophil count. Elevated lysozyrne levels were found in all the inflammatory arthritides and also in osteoarthritis. Lactoferrin levels were not increased in osteoarthritis but displayed a close correlation to the extent of the inflammatory response as judged by SF neutrophilia. The ratio of lysozyme to lactoferrin decreased progressively with increasing SF neutrophilia. In vitro experiments showed that lactoferrin is released from neutrophils isochronously with lysozyme and pglucuronidase. Lactoferrin was not found in hyaline cartilage, a tissue known to contain lysozyme. These results are consistent with the belief that SF lysozyme has a major derivation from both cartilage and neutrophils, and that lactoferrin arises only from neutrophils. These find-

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Lack of effect of lysozymes on cartilage proteoglycans

Greenwald

1976

Archives of Biochemistry and Biophysics

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Effects of acute cartilaginous injury on serum and cartilage lysozyme levels

Cited by 6 publications

References 17 publications

Preferential binding of lysozyme to elastic fibres in pulmonary emphysema

Preferential binding of lysozyme to elastic fibres in pulmonary emphysema

Lactoferrin and lysozyme levels in synovial fluid

Lack of effect of lysozymes on cartilage proteoglycans

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